ABSTRACT
The selection of housekeeping genes is critical for gene expression studies. To address this issue, four candidate housekeeping genes, including several commonly used ones, were investigated in oral squamous cell carcinoma cell lines. A simple quantitative RT-PCR approach was employed by comparing relative expression of the four candidate genes within two cancerous cell lines (HN6 and HN31) and one noncancerous cell line (HaCaT) treated or not with EGF and TGF-beta1. Data were analyzed using ANOVA followed by the NormFinder software program. On this basis, stability of the candidate housekeeping genes was ranked and non statistical differences were found using ANOVA test. On the other hand, the NormFinder was able to show that GAPD and TUBB presented the less variable results, representing appropriated housekeeping genes for the samples and conditions analyzed. In conclusion, this study suggests that the GAPD and the TUBB represent adequate normalizers for gene profiling studies in OSCC cell lines, covering, respectively, high and low expression levels genes.
Subject(s)
Carcinoma, Squamous Cell/genetics , Gene Expression Profiling/methods , Glyceraldehyde-3-Phosphate Dehydrogenases/genetics , Mouth Neoplasms/genetics , Reverse Transcriptase Polymerase Chain Reaction/methods , Tubulin/genetics , Cell Line, Tumor , Humans , Reference Standards , Reverse Transcriptase Polymerase Chain Reaction/standardsABSTRACT
We present the case of a 65-year-old woman who had a painless mass in the left buccal mucosa. Histology showed a benign osteolipoma.
Subject(s)
Lipoma/pathology , Mouth Mucosa/pathology , Mouth Neoplasms/pathology , Ossification, Heterotopic/pathology , Rare Diseases/pathology , Aged , Female , HumansABSTRACT
Hemophilic pseudotumor of bone is a rare condition that occurs in patients with hemophilia. To date only 10 cases have been reported in the mandible, and none has been reported in the maxilla. The clinical, radiographic, and histologic features and treatment approaches of two cases of hemophilic pseudotumor occurring in the jaws are presented. One of them is probably the first one reported in the maxilla. Both patients had a history of trauma to the area.
Subject(s)
Hematoma/complications , Hemophilia A/complications , Jaw Cysts/etiology , Mandibular Diseases/etiology , Maxillary Diseases/etiology , Child , Humans , Male , Maxillofacial Injuries/complications , Tooth Extraction/adverse effectsABSTRACT
The relationship between the histological grading of malignancy and the expression of vimentin and cytokeratin was studied in 43 cases of oral squamous cell carcinoma. Immunohistochemical analysis was carried out with the avidin-biotin method using monoclonal antibody anti-vimentin, and the peroxidase anti-peroxidase method using the polyclonal antibody anti-cytokeratin. All cases were classified according to the histological malignancy grading system proposed by Anneroth. All of the carcinomas were found to express cytokeratin, while 60.4% expressed vimentin. Vimentin was particularly noted in all tumors scored to have highly malignant cells.
