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1.
Anal Bioanal Chem ; 395(7): 2097-106, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19760190

ABSTRACT

The aim of this study is to develop a method for the non-invasive and in situ identification of organic binders in wall paintings by fiber optic mid-FTIR reflectance spectroscopy. The non-invasive point analysis methodology was set-up working on a wide set of wall painting replicas of known composition and using statistical multivariate methods, in particular principal component analysis (PCA), for the interpretation, understanding, and management of data acquired with reflectance mid-FTIR spectroscopy. Results show that PCA can be helpful in managing and preliminary sorting of the large amount of spectra typically collected during non-invasive measurement campaigns and highlight further avenues for research. The developed PCA model was finally applied to the case of a Renaissance wall painting by Perugino assessing it predictability as compared to the interpretation of the single spectrum.

2.
An Esp Pediatr ; 55(4): 310-4, 2001 Oct.
Article in Spanish | MEDLINE | ID: mdl-11578537

ABSTRACT

BACKGROUND: Urinary tract infection (UTI) is one of the most common infections in infants. It presents certain peculiarities compared with other pediatric age groups in terms of symptomatology, diagnosis and the therapeutic approach employed to prevent sequels. OBJECTIVE: To analyze the epidemiology, clinical and laboratory findings, etiology, diagnosis and treatment of first-time UTI in healthy infants. MATERIAL AND METHODS: Between January and December 1999, we performed a retrospective study of 131 previously healthy infants admitted to our hospital with a diagnosis of first-time UTI. Demographic data, clinical characteristics, urine dipstick, urinalysis and urine culture (vesical catheterization), blood cell count and PCR, kidney ultrasonography, voiding cystourethrogram and DMSA scintigraphy were reviewed. RESULTS: We studied 131 patients (median age: 90 days). In infants younger than 30 days, UTI was more prevalent in males. The most frequent symptom was fever (73.3 %). Seventy-one patients fulfilled the criteria for acute pyelonephritis. The presence of nitrituria was low. Escherichia coli was isolated in 90.1 % of the patients. Voiding cystourethrogram detected vesicoureteric reflux in 18.4 % of the patients. Scintigraphy revealed renal scarring in 15.1 %. No significant correlations were found between renal scarring in late scintigraphy and a diagnosis of acute pyelonephritis and/or alterations in the cystourethrogram. CONCLUSIONS: Fever was the main symptom. E. coli was the most commonly isolated microorganism. Nitrituria had low sensitivity in infants. Ultrasonography had low specificity. Scintigraphy showed the highest sensitivity and specificity in the detection of renal scarring. Predictability improved when scintigraphy was performed a few months after acute infection.


Subject(s)
Urinary Tract Infections , Female , Humans , Infant , Infant, Newborn , Male , Retrospective Studies , Sensitivity and Specificity , Urinary Tract Infections/diagnosis , Urinary Tract Infections/drug therapy , Urinary Tract Infections/epidemiology
3.
An. esp. pediatr. (Ed. impr) ; 55(4): 310-314, oct. 2001.
Article in Es | IBECS | ID: ibc-1862

ABSTRACT

Antecedentes: La infección del tracto urinario (ITU) es una de las infecciones bacterianas más comunes del lactante, que presenta unas peculiaridades respecto a otras edades pediátricas en cuanto a síntomas, diagnóstico y actitud terapéutica para la prevención de secuelas. Objetivo: Analizar la epidemiología, hallazgos clínico-analíticos, etiología y procedimientos diagnóstico-terapéuticos en una primera ITU en el lactante sano. Material y métodos: Estudio retrospectivo de 131 lactantes previamente sanos, ingresados en nuestro hospital por ITU entre enero y diciembre de 1999. Se recogieron filiación, síntomas, tira reactiva, sedimento de orina y urocultivo (sondaje uretral), determinaciones analíticas sanguíneas, ecografía renal, cistouretrografía miccional seriada (CUMS) y gammagrafía renal. Resultados: Se estudiaron 131 pacientes (mediana de edad 90 días). En las edades tempranas (menores de 30 días de vida) predominó el sexo masculino. El motivo de consulta más frecuente fue la fiebre (73,3 %). Un total de 71 pacientes cumplían criterios de pielonefritis aguda (PNA). La presencia de nitrituria fue baja. Se aisló Escherichia coli en el 90,1%. La CUMS detectó reflujo vesicoureteral en el 18,4% de pacientes. En el 15,1% de pacientes se objetivó una cicatriz renal en la gammagrafía. No se encontró una relación estadísticamente significativa entre la detección de alteraciones en la gammagrafía renal tardía y el diagnóstico de PNA y/o alteraciones en la CUMS. Conclusiones: La fiebre fue el motivo principal de consulta. El germen más común fue E. coli. La nitrituria tiene una sensibilidad baja en lactantes. La ecografía es una prueba de reducida especificidad. El método más sensible y específico para la detección de lesiones renales es la gammagrafía renal. La predicibilidad de secuelas mejora si la gammagrafía se retrasa unos meses después de la infección (AU)


Subject(s)
Male , Infant, Newborn , Infant , Female , Humans , Urinary Tract Infections , Sensitivity and Specificity , Retrospective Studies
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