ABSTRACT
Previous studies in women have shown that the antiprogestin mifepristone delays or inhibits folliculogenesis. The purpose of this study was to explore whether a new analogue, CDB-2914, has similar effects on folliculogenesis, ovulation, or on subsequent luteal phase endometrial maturation. Forty-four normally cycling, healthy women recorded urine LH and vaginal bleeding during pre-treatment, treatment, and post-treatment cycles. At a lead follicle diameter of 14-16 mm, a single oral dose (10, 50, 100 mg) of CDB-2914 or placebo was given, and daily ultrasound, oestradiol and progesterone were obtained until follicular collapse; an endometrial biopsy was obtained 5-7 days later. Single doses of CDB-2914 were well tolerated. Mid-follicular CDB-2914 suppressed lead follicle growth, causing a dose-dependent delay in folliculogenesis and suppression of plasma oestradiol. At higher doses, a new lead follicle was often recruited. Although luteinized unruptured follicles were observed at the 100 mg dose, all women had follicular collapse. There was a significant delay in endometrial maturation after CDB-2914 at all doses. The treatment cycle was lengthened by 1-2 weeks in 30% at 100, 27% at 50 and 9% at 10 mg. CDB-2914 altered ovarian and endometrial physiology without major effects on menstrual cyclicity and may have therapeutic utility.
Subject(s)
Contraceptives, Postcoital, Synthetic/pharmacology , Endometrium/drug effects , Menstrual Cycle/drug effects , Norpregnadienes/pharmacology , Ovarian Follicle/drug effects , Adolescent , Adult , Biopsy , Cell Differentiation/drug effects , Dose-Response Relationship, Drug , Endometrium/cytology , Endometrium/pathology , Estradiol/blood , Female , Humans , Luteinizing Hormone/blood , Menstrual Cycle/physiology , Ovarian Cysts , Ovulation/drug effectsABSTRACT
The anion-exchange elution behaviour of alpha-lactalbumin was studied on cross-linked and quaternized polyvinylimidazole, deposited on various high-performance liquid chromatographic supports (porous silica and diol silica). The influence of the nature and thickness of the coating layer on the retention and band-width properties of the protein elution peak was examined by isocratic elution. The retention properties of alpha-lactalbumin were studied from the plot of log k' vs. log([NaCl]), where k' is the capacity factor and [NaCl] the displacer salt concentration in the aqueous phase. The retention depends on the amount of stationary phase deposited on the support, but an increased hydrophobic effect is found when the polymer films do not coat the chromatographic support uniformly. Band broadening of the elution peaks was studied in terms of plots of plate height vs. mobile phase velocity. An important mass-transfer contribution is found, which decreases with increasing k' and increases with the thickness of the coating layer. These effects reveal that the diffusion into the polymer layer is the controlling step of the ion-exchange process with non-uniform polymer layers of large mean thickness.
Subject(s)
Chromatography, Ion Exchange/instrumentation , Imidazoles/chemistry , Lactalbumin/chemistry , Polyvinyls/chemistry , Anions , Kinetics , Polymers , Spectrophotometry, UltravioletABSTRACT
A high-performance liquid chromatographic (HPLC) support was prepared, based on silica beads coated with a beta-cyclodextrin-containing polymer, which allows the elution of solutes in order of their affinity for beta-cyclodextrin. Binding constants were determined from the retention data for drugs eluted with pure buffer on the new support and good agreement was observed with results obtained by the Hummel and Dreyer method, previously used in HPLC studies of drug-protein interactions.
