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1.
Rev. bras. reprod. anim ; 34(1): 5750-57, 2010. ilus
Article in Portuguese | VETINDEX | ID: biblio-1491933

ABSTRACT

A maturação in vitro (MIV) de oócitos caninos tem sido objeto de inúmeros estudos focados no estabelecimento de um protocolo capaz de elevar os índices de maturação à metáfase II (MII), atualmente considerados baixos. Dentre as inúmeras variáveis atualmente pesquisadas, estão aspectos relacionados às doadoras (idade, raça, fase estral) e às condições de cultivo, como composição ideal do meio de maturação, adição de diversas fontes proteicas, hormônios, fatores de crescimento e agentes antioxidantes, sendo que os resultados atuais mostram-se ainda pouco eficientes em elevar consideravelmente as taxas de MII. Tal fato pode justificar-se nas diversas características peculiares da biologia reprodutiva desta espécie. Desta maneira, a presente revisão tem como escopo apresentar os aspectos gerais destas características, bem como uma compilação dos aspectos atualmente estudados para a maturação oocitária canina.


The canine oocyte in vitro maturation (IVM) has been the subject of several studies focused on the establishment of a protocol able to increase the maturation rates to metaphase II (MII), currently considered low. Among the many variables currently researched are aspects related to the donors (age, breed, estrous stage) and to the culture conditions, as the ideal composition of the maturation medium, the addition of several protein resources, hormones, growth factors and anti oxidation factors, being that the current results still present little efficiency in increasing considerably the MII rates. Such fact may be justified in the many peculiar characteristics of the reproductive biology of this specie. Therefore, the current review is aiming to present the general aspects of these characteristics, as well as a compilation of the aspects currently studied for canine oocyte maturation.


Subject(s)
Female , Animals , Adult , Dogs , Metaphase/physiology , Oocytes/transplantation , Embryo Transfer/methods , Embryo Transfer/veterinary
2.
R. bras. Reprod. Anim. ; 34(1): 5750, j2010. ilus
Article in Portuguese | VETINDEX | ID: vti-4615

ABSTRACT

A maturação in vitro (MIV) de oócitos caninos tem sido objeto de inúmeros estudos focados no estabelecimento de um protocolo capaz de elevar os índices de maturação à metáfase II (MII), atualmente considerados baixos. Dentre as inúmeras variáveis atualmente pesquisadas, estão aspectos relacionados às doadoras (idade, raça, fase estral) e às condições de cultivo, como composição ideal do meio de maturação, adição de diversas fontes proteicas, hormônios, fatores de crescimento e agentes antioxidantes, sendo que os resultados atuais mostram-se ainda pouco eficientes em elevar consideravelmente as taxas de MII. Tal fato pode justificar-se nas diversas características peculiares da biologia reprodutiva desta espécie. Desta maneira, a presente revisão tem como escopo apresentar os aspectos gerais destas características, bem como uma compilação dos aspectos atualmente estudados para a maturação oocitária canina.(AU)


The canine oocyte in vitro maturation (IVM) has been the subject of several studies focused on the establishment of a protocol able to increase the maturation rates to metaphase II (MII), currently considered low. Among the many variables currently researched are aspects related to the donors (age, breed, estrous stage) and to the culture conditions, as the ideal composition of the maturation medium, the addition of several protein resources, hormones, growth factors and anti oxidation factors, being that the current results still present little efficiency in increasing considerably the MII rates. Such fact may be justified in the many peculiar characteristics of the reproductive biology of this specie. Therefore, the current review is aiming to present the general aspects of these characteristics, as well as a compilation of the aspects currently studied for canine oocyte maturation.


Subject(s)
Animals , Female , Adult , Dogs , Embryo Transfer/methods , /trends , Embryo Transfer/veterinary , Oocytes/transplantation , Metaphase/physiology
3.
J Small Anim Pract ; 48(12): 713-5, 2007 Dec.
Article in English | MEDLINE | ID: mdl-18005109

ABSTRACT

This report presents the case of a nine-month-old bitch with incomplete occlusion of the skin and mucosa between the anus and dorsal commissure of the vulva. This anomaly, known as anovulvar cleft, was associated with a vaginal prolapse-hyperplasia. Anovulvar cleft is a rare condition in bitches and is caused by incomplete closure of the dorsal urogenital folds. Anovulvar cleft can lead to clitoritis, abrasion, dryness and devitalisation of the smooth exposed mucosa. Surgery can correct the condition. In the present study, surgical resection of the hyperplasic vaginal tissue associated with perineoplasty using an inverted V-shaped incision showed excellent results. An ovariohysterectomy was also performed to prevent recurrence of the vaginal prolapse-hyperplasia.


Subject(s)
Dog Diseases/diagnosis , Hyperplasia/veterinary , Uterine Prolapse/veterinary , Animals , Animals, Newborn , Diagnosis, Differential , Dog Diseases/pathology , Dog Diseases/surgery , Dogs , Female , Gynecologic Surgical Procedures/veterinary , Hyperplasia/diagnosis , Uterine Prolapse/diagnosis
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