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1.
Br J Nutr ; 131(10): 1691-1698, 2024 May 28.
Article in English | MEDLINE | ID: mdl-38221826

ABSTRACT

Ultra-processed plant-based foods, such as plant-based burgers, have gained in popularity. Particularly in the out-of-home (OOH) environment, evidence regarding their nutritional profile and environmental sustainability is still evolving. Plant-based burgers available at selected OOH sites were randomly sampled in Amsterdam, Copenhagen, Lisbon and London. Plant-based burgers (patty, bread and condiment) (n 41) were lab analysed for their energy, macronutrients, amino acids and minerals content per 100 g and serving and were compared with reference values. For the plant-based burgers, the median values per 100 g were 234 kcal, 20·8 g carbohydrates, 3·5 g dietary fibre and 12·0 g fat, including 0·08 g TFS and 2·2 g SFA. Protein content was 8·9 g/100 g, with low protein quality according to amino acid composition. Median Na content was 389 mg/100 g, equivalent to 1 g salt. Compared with references, the median serving provided 31% of energy intake based on a 2000 kcal per day and contributed to carbohydrates (17-28%), dietary fibre (42%), protein (40%), total fat (48%), SFA (26%) and Na (54%). One serving provided 15-23% of the reference values for Ca, K and Mg, while higher contributions were found for Zn, Mn, P and Fe (30-67%). The ultra-processed plant-based burgers provide protein, dietary fibre and essential minerals and contain relatively high levels of energy, Na and total fats. The amino acid composition indicated low protein quality. The multifaceted nutritional profile of plant-based burgers highlights the need for manufacturers to implement improvements to better support healthy dietary habits, including reducing energy, Na and total fats.


Subject(s)
Dietary Fiber , Energy Intake , Nutritive Value , Dietary Fiber/analysis , Humans , Amino Acids/analysis , Dietary Proteins/analysis , Nutrients/analysis , Food Handling/methods , Minerals/analysis , Dietary Fats/analysis , Dietary Carbohydrates/analysis , Fast Foods/analysis , Bread/analysis
2.
Sci Rep ; 13(1): 8458, 2023 05 25.
Article in English | MEDLINE | ID: mdl-37231055

ABSTRACT

Immune reconstitution after hematopoietic stem cell transplantation (HSCT) is a complex and extremely variable process. The Ikaros transcription factor plays an important role in hematopoiesis in several cell lines, especially in the lymphoid lineage. We hypothesized that Ikaros might influence immune reconstitution, and consequently, the risk of opportunistic infections, relapse, and graft versus host disease (GVHD). Samples were collected from the graft and from the peripheral blood (PB) of the recipients 3 weeks after neutrophil recovery. Real-time polymerase chain reaction (RT-PCR) was performed to analyze the absolute and relative Ikaros expression. Patients were divided into two groups, according to Ikaros expression in the graft and in the recipients' PB based on the ROC curves for moderate/severe cGVHD. A cutoff of 1.48 was used for Ikaros expression in the graft, and a cutoff of 0.79 was used for Ikaros expression in the recipients' PB. Sixty-six patients were included in this study. Median age of patients was 52 years (range 16-80 years), 55% of them were male, and 58% of them had acute leukemia. Median follow-up period was 18 months (range 10-43 months). There was no association between Ikaros expression and the risk of acute GVHD, relapse, or mortality. However, a significant association was observed with the risk of chronic GVHD. Higher Ikaros expression in the graft was associated with a significantly higher cumulative incidence (CI) of moderate/severe chronic GVHD according to the National Institute of Health (NIH) classification at two years (54% vs. 15% for patients with lower expression, P = 0.03). A higher Ikaros expression in the recipients' PB 3 weeks after engraftment was also associated with a significantly higher risk of moderate/severe chronic GVHD (65% vs. 11%, respectively, P = 0.005). In conclusion, Ikaros expression in the graft and in the recipients' PB after transplantation was associated with a higher risk of moderate/severe chronic GVHD. Ikaros expression should be evaluated in larger prospective trials as a potential biomarker for chronic GVHD.


Subject(s)
Bronchiolitis Obliterans Syndrome , Graft vs Host Disease , Hematopoietic Stem Cell Transplantation , Ikaros Transcription Factor , Leukemia, Myeloid, Acute , Adolescent , Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Young Adult , Chronic Disease , Graft vs Host Disease/etiology , Hematopoietic Stem Cell Transplantation/adverse effects , Leukemia, Myeloid, Acute/etiology , Prospective Studies , Recurrence , Ikaros Transcription Factor/genetics , Ikaros Transcription Factor/metabolism
3.
Arq. bras. med. vet. zootec. (Online) ; 73(5): 1001-1013, Sept.-Oct. 2021. tab, ilus
Article in English | LILACS, VETINDEX, HomeoIndex Homeopathy | ID: biblio-1345278

ABSTRACT

The goal of this research was to compare the effect of the following products on dairy cattle, parasitized by Rhipicephalus microplus: organosynthetics - Clorfenvinfós and Ivermectina (OG); phytotherapics - Eucalyptus oil (EG) and Neem cake (NG); Homeopathy (HG); The control group (CG) received no treatment. Infestation was by R. microplus (MIC) and weight gain (GP) were evaluated. The study included 60 animals (5 groups) from ­ Dutch / Zebu, aged between 25-44 months and initial weight between 211-477kg. EG and NG showed MIC 84.9% and 14.0% greater than CG, respectively (P <0.05; P = 0). HG and OG had MIC less than CG 24.4% and 16.9%, respectively (P<0.05; P <0.05). EG, NG and OG obtained GP lower than CG in 7.9%, 8.9% and 8.06% (P <0.05) respectively. The HG GP was 4.9% higher (P <0.05) than CG. This was the first research to prove parasitism control by R. microplus in a field test, using homeopathy. The methodology for choosing Homeopathy for the control of enzooties, developed and tested for the first time in this study, proved to be adequate and efficient, opening the possibility for establishing a new methodology for strategic control of parasitism by R. microplus.(AU)


O objetivo desta pesquisa foi comparar o efeito dos seguintes produtos sobre bovinos leiteiros, parasitados por Rhipicephalus microplus: organossintéticos - clorfenvinfós e ivermectina (OG); fitoterápicos - óleo de eucalipto (EG) e torta de Neem (NG) e homeopatia (HG). O grupo controle (CG) não recebeu tratamento. Foram avaliados infestação por R. microplus (MIC) e ganho de peso/grupo (GP) em 60 animais (5 grupos), a partir de ­ holandês/zebu, com idade entre 25 e 44 meses e peso inicial entre 211 e 477kg. EG e NG apresentaram MIC 84,9% e 14,0% maior que CG, respectivamente (P<0,05; P=0). HG e OG apresentaram MIC menor que CG 24,4% e 16,9%, respectivamente (P<0,05; P<0,05). EG, NG e OG obtiveram GP menor que CG em 7,9%, 8,9% e 8,06% (P<0,05), respectivamente. O GP do HG foi 4,9% maior (P<0,05) que o CG. Esta foi a primeira pesquisa a comprovar controle do parasitismo por R. microplus em teste a campo, usando homeopatia. A metodologia para escolha de medicamentos homeopáticos para controle de enzootias, desenvolvida e testada pela primeira vez neste estudo, mostrou-se adequada e eficiente, abrindo a possibilidade para estabelecimento de nova metodologia para controle estratégico do parasitismo por R. microplus.(AU)


Subject(s)
Animals , Female , Cattle , Ticks , Homeopathic Remedy , Tick Control/methods , Acaricides
4.
Heliyon ; 7(4): e06771, 2021 Apr.
Article in English | MEDLINE | ID: mdl-33912724

ABSTRACT

The application of sewage sludge in soils can bring benefits to agricultural productivity, however, the risks arising from this application must be measured and carefully evaluated. Persistent organics compounds and drugs can be present in the sewage sludge and if applied to the soil, they can cause severe risks to the soil biota and contamination of groundwater. This work determined 174 persistent organic compounds and drugs in sludge samples from a wastewater treatment plant using chromatographic methods. The drugs ciprofloxacin, enrofloxacin and diclofenac were quantified, and values varied according to sampling period. For persistent organic compounds, cresols were the most abundant compounds in sewage sludge. With the analyses made of both the elutriate and the filtrate, it was possible to verify the potential for soil retention or leaching that each compound can present with the application of sewage sludge in the agriculture.

5.
J Endocrinol Invest ; 44(10): 2295-2305, 2021 Oct.
Article in English | MEDLINE | ID: mdl-33730348

ABSTRACT

INTRODUCTION: Thyrotropin stimulating hormone (TSH) suppression in patients with differentiated thyroid cancer (DTC) aims to decrease the growth and proliferation of thyroid cancer cells. However, the effect of TSH-suppressive therapy on bone microarchitecture remains undefined. METHODS: Cross-sectional study including 43 women with DTC undergoing TSH-suppressive therapy (sTSH) compared to 20 women also on levothyroxine (LT4) therapy but with TSH in the low-normal range (nTSH) since the thyroid surgery. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry (DXA), and trabecular bone score (TBS) was evaluated using the TBS iNsigth software. Fracture risk assessed by FRAX, with and without TBS, was calculated. The relationship between suppressive therapy-related parameters and bone parameters was investigated. RESULTS: The TBS mean values were not significantly different in the sTSH and nTSH groups (1.273 ± 0.12 vs 1.307 ± 0.14, p = 0.7197). In both groups, postmenopausal women had degraded microarchitecture (TBS 1.216 ± 0.11 vs 1.213 ± 0.09, p = 0.9333), while premenopausal women had normal microarchitecture (1.328 ± 0.11 vs 1.401 ± 0.12, p = 0.195). The percentage of all postmenopausal women with degraded TBS was 54.7%, while the percentage of osteoporosis diagnoses was 16.1%. The TBS-adjusted FRAX-probability of fracture was similar in sTSH and nTSH groups. Body mass index (BMI) and menopausal status were the only variables associated with TBS and BMD. CONCLUSION: Trabecular microarchitecture assessed by TBS was similar between women on long-term suppressive therapy in DTC and those on LT4 replacement therapy aiming at a TSH level within the low-normal reference range. Low TBS values were observed in postmenopausal women of both groups, suggesting that not only suppressed TSH levels but also a low-normal TSH is associated with deteriorated bone microarchitecture in postmenopausal women following total thyroidectomy.


Subject(s)
Adenocarcinoma/drug therapy , Bone Density , Cancellous Bone/pathology , Osteoporosis/pathology , Thyroid Neoplasms/drug therapy , Thyrotropin/antagonists & inhibitors , Thyroxine/adverse effects , Adenocarcinoma/pathology , Adolescent , Adult , Aged , Aged, 80 and over , Brazil/epidemiology , Cancellous Bone/drug effects , Cross-Sectional Studies , Female , Follow-Up Studies , Humans , Menopause , Middle Aged , Osteoporosis/chemically induced , Osteoporosis/epidemiology , Prognosis , Thyroid Neoplasms/pathology , Young Adult
6.
J Investig Allergol Clin Immunol ; 31(3): 236-245, 2021 Jun 22.
Article in English | MEDLINE | ID: mdl-31932275

ABSTRACT

BACKGROUND: Atopic dermatitis is a chronic inflammatory skin disease with a prevalence of 0.02% to 8.1% in adults. Adult patients with moderate-to-severe atopic dermatitis are affected by frequent relapses and a significant disease burden. Objective: To determine the clinical, immunological, and therapeutic profile of Brazilian adults with atopic dermatitis. METHODS: A multicenter, observational, retrospective, descriptive registry-based study was conducted at reference hospitals between December 2016 and October 2017. The data collected were demographics, personal and family history of atopic diseases, clinical manifestations, laboratory tests, disease severity and management. RESULTS: Of the 187 patients included in the analysis, 56.1% were female and 71.7% were White, with a mean age of 24.7 years. Mean follow-up was 9 years. Asthma or other allergic diseases were reported by 80.2% of patients. The main comorbidity was hypertension (10.2%), and common disease manifestations included pruritus and erythema. Lesions generally affected flexural and nonflexural areas, with typical morphology. Around 83% of patients had moderate-to-severe disease, and 8.6% reported at least 1 hospitalization. Most patients received topical and/or systemic pharmacological therapies, including omalizumab (5.9%); 4.3% received phototherapy. Moreover, 66.8% of patients received adjuvant therapy, and 79.1% changed or discontinued treatment for atopic dermatitis due to remission (46.5%), poor effectiveness (33.7%), or lack of adherence (12.9%). Most patients presented characteristics of type 2 inflammation, with immunoglobulin E levels above 100 IU/mL (94.4%) and peripheral blood eosinophils above 5% (55.9%). CONCLUSION: Brazilian adult patients with severe atopic dermatitis need treatment to efficiently control the disease and improve quality of life.


Subject(s)
Dermatitis, Atopic/immunology , Eosinophils/immunology , Hypertension/epidemiology , Omalizumab/therapeutic use , Adult , Brazil/epidemiology , Comorbidity , Demography , Dermatitis, Atopic/drug therapy , Dermatitis, Atopic/epidemiology , Disease Progression , Erythema , Female , Follow-Up Studies , Hospitalization/statistics & numerical data , Humans , Immunoglobulin E/blood , Male , Pruritus , Tertiary Care Centers
7.
Platelets ; 32(3): 368-377, 2021 Apr 03.
Article in English | MEDLINE | ID: mdl-32248725

ABSTRACT

Vascular complications resulting from atherosclerosis development are a major cause of death. Reactive oxygen species (ROS) are produced by platelets during activation, and have been demonstrated to positively regulate platelet activatory responses. Zn2+ is also an important hemostatic cofactor in platelets, acting both as a platelet agonist and second messenger. Whilst the effect of Zn2+-dependent signaling mechanisms on ROS production in nucleated cells has been demonstrated, comparable roles in platelets have yet to be investigated. In this study we investigated the relationship between fluctuations in cytosolic Zn2 [Zn2+]i and platelet ROS production. Agonist-evoked ROS production, GSH levels and GPx activity are abrogated in platelets treated with the Zn2+-chelator, TPEN. Conversely, increasing platelet [Zn2+]i using Zn2+ ionophores potentiated ROS generation and decreased GSH levels and GPx activity. Zn2+-dependent ROS production was sensitive to pretreatment with DPI or mitoTEMPO, NADPH oxidase and mitochondria inhibitors respectively. Increasing [Zn2+]i resulted in increases of Erk1/2 and JNK phosphorylation. Our data are consistent with a functional association between [Zn2+]i and ROS production in platelets that could influence thrombus formation in a clinical context.


Subject(s)
Blood Platelets/metabolism , Zinc/therapeutic use , Animals , Humans , Reactive Oxygen Species
8.
J. investig. allergol. clin. immunol ; 31(3): 236-245, 2021. tab, graf
Article in English | IBECS | ID: ibc-215205

ABSTRACT

Background: Atopic dermatitis is a chronic inflammatory skin disease with a prevalence of 0.02% to 8.1% in adults. Adult patients with moderate-to-severe atopic dermatitis are affected by frequent relapses and a significant disease burden. Objective: To determine the clinical, immunological, and therapeutic profile of Brazilian adults with atopic dermatitis. Methods: A multicenter, observational, retrospective, descriptive registry-based study was conducted at reference hospitals between December 2016 and October 2017. The data collected were demographics, personal and family history of atopic diseases, clinical manifestations, laboratory tests, disease severity and management. Results: Of the 187 patients included in the analysis, 56.1% were female and 71.7% were White, with a mean age of 24.7 years. Mean follow-up was 9 years. Asthma or other allergic diseases were reported by 80.2% of patients. The main comorbidity was hypertension (10.2%), and common disease manifestations included pruritus and erythema. Lesions generally affected flexural and nonflexural areas, with typical morphology. Around 83% of patients had moderate-to-severe disease, and 8.6% reported at least 1 hospitalization. Most patients received topical and/or systemic pharmacological therapies, including omalizumab (5.9%); 4.3% received phototherapy. Moreover, 66.8% of patients received adjuvant therapy, and 79.1% changed or discontinued treatment for atopic dermatitis due to remission (46.5%), poor effectiveness (33.7%), or lack of adherence (12.9%). Most patients presented characteristics of type 2 inflammation, with immunoglobulin E levels above 100 IU/mL (94.4%) and peripheral blood eosinophils above 5% (55.9%). Conclusion: Brazilian adult patients with severe atopic dermatitis need treatment to efficiently control the disease and improve quality of life (AU)


Subject(s)
Humans , Male , Female , Young Adult , Adult , Dermatitis, Atopic/immunology , Eosinophils/immunology , Omalizumab/therapeutic use , Anti-Allergic Agents/therapeutic use , Retrospective Studies , Brazil/epidemiology , Comorbidity , Dermatitis, Atopic/drug therapy , Dermatitis, Atopic/epidemiology , Disease Progression , Follow-Up Studies , Hospitalization/statistics & numerical data , Immunoglobulin E/blood , Tertiary Healthcare
9.
Arq. bras. med. vet. zootec. (Online) ; 72(6): 2259-2265, Nov.-Dec. 2020. ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1142302

ABSTRACT

A osteomielite é um desafio terapêutico em ortopedia, capaz de retardar ou mesmo impedir a consolidação óssea. O omento, há anos, tem sido empregado como alternativa em diferentes procedimentos cirúrgicos, por sua capacidade, entre outras, de angiogênese, sendo aplicado na ortopedia veterinária quando há o risco de não união óssea. Neste caso, um cão Fila Brasileiro foi submetido à realização de enxerto com retalho pediculado de omento maior, após osteomielite resistente presente em osteossíntese de fratura múltipla de tíbia aberta grau II. Durante 16 dias, manteve-se a comunicação do retalho, mas, diante do risco de peritonite, o pedículo foi seccionado. Numa sequência de intervenções cirúrgicas, após 89 dias, houve cicatrização óssea e remissão da osteomielite, mesmo na presença de bactérias multirresistentes. Neste relato, o omento foi efetivo como terapia adjuvante no tratamento da osteomielite e garantiu o retorno da função do membro.(AU)


Osteomyelitis is a therapeutic challenge in orthopedics, capable of delaying or even preventing bone healing. The omentum has been used in different surgical procedures as an alternative for its capacity, among others, of angiogenesis, being applied in veterinary orthopedics, when there is a risk of non-union of bone. In this case, a Brazilian row dog was submitted to grafting with pedicle flap of greater omentum, after resistant osteomyelitis present in open fracture osteosynthesis of open tibia grade II. For 16 days the communication of the flap was maintained, but at the risk of peritonitis, the pedicle was sectioned. In a sequence of surgical interventions, after 89 days, there was bone healing and remission of osteomyelitis, even in the presence of multi-resistant bacteria. In this report, the omentum was effective as adjuvant therapy in the treatment of osteomyelitis and guaranteed the return of limb function.(AU)


Subject(s)
Animals , Dogs , Omentum/transplantation , Osteomyelitis/therapy , Osteomyelitis/veterinary , Tibia/pathology , Pedicle Screws/veterinary
10.
Top Companion Anim Med ; 41: 100459, 2020 Nov.
Article in English | MEDLINE | ID: mdl-32823158

ABSTRACT

A pregnant 2-year-old mixed-breed dog was admitted with a 2-day history of lethargy, anorexia and painful abdominal distension. Clinical manifestations were unspecific and mainly suggested hypovolemic shock. Physical examination, ultrasonographic evaluation and radiographs confirmed mid-late pregnancy and evident signs of fetal death and peritoneal effusion. An exploratory laparotomy was immediately initiated which revealed torsion of the right gravid uterine horn over the left one and, simultaneously, the unexpected presence of splenic torsion and rupture, with a stretched omentum covering and exerting tension on the spleen's cranial pole. Histopathology revealed hemorrhagic infarction of the uterus and spleen. The animal recovered uneventfully and was later submitted to a preventive gastropexy.


Subject(s)
Dog Diseases/diagnosis , Splenic Diseases/veterinary , Torsion Abnormality/veterinary , Uterine Diseases/veterinary , Animals , Dogs , Female , Fetal Death , Laparotomy/veterinary , Pregnancy
11.
Phys Rev E ; 102(1-1): 012104, 2020 Jul.
Article in English | MEDLINE | ID: mdl-32794977

ABSTRACT

We analyze a set of discrete-time quantum walks for which the displacements on a chain follow binary aperiodic jumps according to three paradigmatic sequences: Fibonacci, Thue-Morse, and Rudin-Shapiro. We use a generalized Hadamard coin, C[over ̂]_{H}, as well as a generalized Fourier coin, C[over ̂]_{K}. We verify the QW experiences a slowdown of the wave packet spreading, σ^{2}(t)∼t^{α}, by the aperiodic jumps whose exponent, α, depends on the type of aperiodicity. Additional aperiodicity-induced effects also emerge, namely, (1) while the superdiffusive regime (1<α<2) is predominant, α displays an unusual sensibility with the type of coin operator where the more pronounced differences emerge for the Rudin-Shapiro and random protocols and (2) even though the angle θ of the coin operator is homogeneous in space and time, there is a nonmonotonic dependence of α with θ. Fingerprints of the aperiodicity in the hoppings are also found when distributional measures such as the Shannon and von Neumann entropies, the Inverse Participation Ratio, the Jensen-Shannon dissimilarity, and the kurtosis are computed, which allow assessing informational and delocalization features arising from these protocols and understanding the impact of linear and nonlinear correlations of the jump sequence in a quantum walk as well. Finally, we argue the spin-lattice entanglement is enhanced by aperiodic jumps.

12.
J Mycol Med ; 30(4): 101010, 2020 Dec.
Article in English | MEDLINE | ID: mdl-32690370

ABSTRACT

The aim of this research is to verify the yeast species isolated from oral mucosa in street mixed-breed dogs and to determine the antifungal profiles. After capturing and sedating the animals, oral mucosa samples were collected from fifty dogs and the materials were inoculated on Sabouraud dextrose agar with chloramphenicol. Forty-three yeast strains were isolated and identified trough the API-20C AUX method. Thirty-seven (86.1%) of the yeasts belonged to genus Candida, five (11.6%) to genus Trichosporon and only one strain (2.3%) to genus Malassezia. The sensitivity profiles to anifungals (amphotericin B, itraconanole, ketoconazole, fluconazole and variconazole) were determined through Etest® method. This study found resistance of some yeasts to amphotericin B and a good susceptibility to voriconazole and ketoconazole. Some of these antifungals are used in veterinary medical practice. This research is the first investigation on street mixed-breed dogs regarding yeast identifications and antifungals profiles.


Subject(s)
Antifungal Agents/pharmacology , Dogs/microbiology , Mouth Mucosa/microbiology , Yeasts/drug effects , Yeasts/isolation & purification , Animals , Animals, Wild , Crosses, Genetic , Dog Diseases/microbiology , Dogs/genetics , Drug Resistance, Fungal , Female , Male , Microbial Sensitivity Tests , Mycoses/microbiology , Mycoses/veterinary
13.
Arq. bras. med. vet. zootec. (Online) ; 72(2): 493-498, Mar./Apr. 2020. ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1128385

ABSTRACT

Foi atendida, no Hospital Veterinário da Universidade de Cuiabá, uma coruja suindara (Tyto furcata), resgatada pela Polícia Militar Ambiental, sem histórico, adulta e 400 gramas de peso corporal. A ave apresentava impotência funcional do membro pélvico direito, além de desvio, crepitação e dor na região proximal tibiotársica direita, sem lesão cutânea. Estudo radiográfico revelou fratura diafisária oblíqua curta em tibiotarso direito. A estabilização da fratura foi realizada com placa bloqueada do sistema de 1,5mm, na função de apoio, seis orifícios, com três parafusos monocorticais distais, dois parafusos monocorticais proximais e um parafuso bicortical proximal. Ao 30º dia do pós-operatório, os achados radiográficos de consolidação óssea e o retorno da função do membro permitiram a alta médica. Concluiu-se que o uso de placa bloqueada em função apoio para estabilização de fraturas tibiotársicas simples de coruja suindara oferece resultados satisfatórios.(AU)


An adult suindara owl (Tyto furcata), with no specific history and 400 grams of body weight was treated at the Veterinary Hospital of the University of Cuiabá. The patient presented functional impotence in the right pelvic limb, deviation, crepitation and pain in the proximal region in right tibiotarsal, without cutaneous lesions. Radiographs revealed a short oblique diaphyseal fracture at the right tibiotarsus. Fracture stabilization was performed with a 1.5mm locking plate, buttress, six holes; three distal monocortical screws, two proximal monocortical screws and a proximal bicortical screw. On the 30th postoperative day, radiographic revealed bone healing, and the return of limb function allowed medical discharge. It was concluded that the use of locking plates in buttress function to stabilize simple tibiotarsus fractures in suindara owl offers satisfactory results.(AU)


Subject(s)
Animals , Tibial Fractures/surgery , Bone Plates/veterinary , Strigiformes/surgery , Fractures, Bone/veterinary , Fracture Fixation, Internal/veterinary
14.
Photodiagnosis Photodyn Ther ; 28: 216-220, 2019 Dec.
Article in English | MEDLINE | ID: mdl-31479804

ABSTRACT

BACKGROUND: Laser-assisted MAL-PDT has been reported to increase the effectiveness of conventional PDT. Nonetheless, clinical effects of this association when reducing MAL incubation time is poorly discussed. Furthermore, the association of acoustic pressure wave ultrasound with laser-assisted MAL-PDT with short incubation time for field cancerization had not been reported before. OBJECTIVES: To compare clinical effects of ablative fractional laser-assisted MAL-PDT associated with acoustic pressure wave ultrasound (IMPACT US) with 1-hour incubation time and conventional MAL-PDT for skin field cancerization on the forearms, as well as the impact on safety and tolerability. METHODS: Fifteen patients with 638 AK (grade I-III) with field cancerized-skin on the forearms were enrolled in this left-right trial. Two protocols were randomly chosen. One side was treated with conventional MAL-PDT, whereas the other with laser-assisted MAL-PDT associated with acoustic pressure wave ultrasound with 1-hour incubation time. Actinic keratoses were quantitively measured, and the other signs of sun-damaged skin, like pigmentation and texture, in field cancerized skin were qualitatively evaluated before and after six months. Side effects were assessed subjectively during the procedure and one week after. RESULTS: All patients completed the study. At six months after treatment, both protocols reduced the number of AK (72%; CO2 + PDT, and 65%; MAL-PDT). The difference between these two protocols was not statistically significant (p = 0.77). The improvement of pigmentation and texture of field cancerized skin was more significant on the side treated with laser-assisted MAL-PDT associated with acoustic pressure wave ultrasound. Both protocols were well tolerated and without significant difference in adverse events. CONCLUSION: Laser-assisted MAL-PDT using CO2 laser and acoustic pressure wave ultrasound with short incubation time of 1 h was as effective as conventional MAL-PDT for field-cancerized skin with actinic keratosis in forearms with better cosmetic outcome.


Subject(s)
Aminolevulinic Acid/analogs & derivatives , Elasticity Imaging Techniques/methods , Keratosis, Actinic/therapy , Lasers, Gas/therapeutic use , Photosensitizing Agents/therapeutic use , Skin Neoplasms/therapy , Adult , Aged , Aminolevulinic Acid/therapeutic use , Combined Modality Therapy , Female , Forearm , Humans , Keratosis, Actinic/drug therapy , Male , Middle Aged , Photochemotherapy , Skin Neoplasms/drug therapy , Ultrasonic Waves
15.
Thromb Res ; 172: 44-50, 2018 12.
Article in English | MEDLINE | ID: mdl-30359790

ABSTRACT

INTRODUCTION: Tumor necrosis factor-alpha (TNF-α) exerts a critical role in inflammatory events through two distinct receptors, TNFR1 and TNFR2. Platelets have been recognized as important inflammatory cells, but little is known about the effects of TNF-α on the platelet activity. OBJECTIVES: In the present study we have studied the role of TNF-α on ADP-induced platelet aggregation and its downstream signaling (c-Src and fibrinogen receptor phosphorylation, cytosolic Ca2+ mobilization, cAMP and cGMP levels and cell viability). METHODS AND RESULTS: Washed rat platelets were incubated with TNF-α (1-3000 pg/ml) for different time-periods (5-60 min) before the addition of ADP (5 µM) to induce platelet aggregation. TNF-α concentration- and time-dependently inhibits ADP-induced aggregation, which was significantly prevented by incubation with the non-selective TNF-α receptor antagonist R7050. TNF-α (300 pg/ml, 30 min) decreases thrombin-induced elevation of cytosolic Ca++ levels by 2.2- fold compared to untreated platelets. TNF-α decreases the cAMP levels, while significantly increases the intracellular cyclic cGMP levels. However, the pre-incubation of platelets with the guanylyl cyclase inhibitor ODQ, despite decreasing the cGMP levels, does not modify the inhibitory effect of TNF-α on ADP-induced platelet aggregation. Additionally, western blotting analysis showed that TNF-α significantly reduced (Tyr 416)-c-Src and (Tyr773)-ß3 subunit of αIIbß3 integrin phosphorylation. TNF-α does not affect the platelet viability in any condition tested. CONCLUSION: Therefore, our results show that TNF-α negatively modulates ADP-induced aggregation via TNFR1/TNFR2 receptors by reducing cytosolic Ca++ levels and by inhibiting c-Src and fibrinogen receptor activation, which take place through cAMP- and cGMP-independent mechanisms.


Subject(s)
Blood Platelets/metabolism , Calcium/metabolism , Integrin beta3/metabolism , Platelet Aggregation , Platelet Glycoprotein GPIIb-IIIa Complex/metabolism , Platelet Membrane Glycoprotein IIb/metabolism , Tumor Necrosis Factor-alpha/metabolism , Animals , Blood Platelets/cytology , Cyclic GMP/metabolism , Cytosol/metabolism , Male , Phosphorylation , Rats, Wistar
16.
Food Funct ; 9(8): 4419-4428, 2018 Aug 15.
Article in English | MEDLINE | ID: mdl-30066000

ABSTRACT

Cancers induced by human papillomavirus (HPV) infection remain a significant public health threat, fueling the study of new therapies. Laurel (Laurus nobilis) compounds and extracts recently showed in vitro activity against HPV-transformed cell lines. This work aims to evaluate the in vivo efficacy and hepatic toxicity of a laurel extract in a transgenic mouse model of HPV16-induced cancer. The extract was administered in drinking water (20 mg per animal per day) for three consecutive weeks, using four experimental groups (n = 10) (group I: HPV16-/- without treatment, group II: treated HPV16-/-, group III: HPV16+/- without treatment and group IV: treated HPV16+/-). Following the treatment period, animals were sacrificed and skin samples were used to classify skin lesions histologically. Toxicological parameters included hematological and biochemical blood markers, splenic and hepatic histology and hepatic oxidative stress. The extract did not prevent the progression of HPV16-induced cutaneous lesions in this model. The treated wild-type animals showed mild hepatitis, while transgenic animals suffered weight loss. However, there were no changes concerning hematological, biochemical and hepatic oxidative stress markers.


Subject(s)
Antineoplastic Agents, Phytogenic/toxicity , Human papillomavirus 16/physiology , Laurus/chemistry , Papillomavirus Infections/virology , Plant Extracts/toxicity , Uterine Cervical Neoplasms/virology , Animals , Antineoplastic Agents, Phytogenic/administration & dosage , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Female , Human papillomavirus 16/genetics , Humans , Liver/drug effects , Liver/metabolism , Liver/pathology , Mice , Mice, Transgenic , Oxidative Stress/drug effects , Papillomavirus Infections/metabolism , Papillomavirus Infections/pathology , Plant Extracts/administration & dosage , Plant Extracts/chemistry , Plant Extracts/isolation & purification , Uterine Cervical Neoplasms/metabolism , Uterine Cervical Neoplasms/pathology
19.
Biomed Pharmacother ; 104: 275-279, 2018 Aug.
Article in English | MEDLINE | ID: mdl-29775895

ABSTRACT

Infection with high-risk human papillomavirus (HPV), most often HPV16, is associated with the development of anogenital and oropharyngeal cancers. Recently, ozone therapy was reported to have considerable efficacy against rabbit VX2 tumors, induced by the cottontail rabbit papillomavirus. The present study aims to determine whether similar results can be obtained in HPV16-transgenic mice, possibly paving the way for new therapeutic options against HPV-induced cancers. HPV16-transgenic and wild-type, female, 20 weeks-old mice were injected intraperitoneally with medical O3/O2 (80░mL/kg, at O3 50░µg/mL), once a day, for 5 consecutive days. The animals were sacrificed at 25 weeks-old, and skin samples were analyzed histologically to study tumour progression. Blood and internal organ samples were used to study toxicological parameters. 85.7% of untreated transgenic mice showed dysplastic skin lesions, compared with 28.6% of O3-treated mice. This was associated with a marked reduction of dermal inflammation associated with those lesions. No significant changes were observed in any toxicological parameters. These preliminary results support the hypothesis that O3 therapy is effective against papillomavirus-induced lesions, particularly against those induced by the most common high-risk virus, HPV16. Further studies are needed to confirm the mechanisms underlying these effects.


Subject(s)
Human papillomavirus 16/pathogenicity , Neoplasms/drug therapy , Ozone/pharmacology , Skin Diseases/drug therapy , Animals , Disease Progression , Female , Mice , Mice, Transgenic , Neoplasms/virology , Papillomavirus Infections/complications , Rabbits , Skin/drug effects , Skin/virology , Skin Diseases/virology , Treatment Outcome
20.
Neuropathol Appl Neurobiol ; 44(3): 298-313, 2018 04.
Article in English | MEDLINE | ID: mdl-29044639

ABSTRACT

AIMS: The association between the pathological features of AD and dementia is stronger in younger old persons than in older old persons suggesting that additional factors are involved in the clinical expression of dementia in the oldest old. Cumulative data suggests that neuroinflammation plays a prominent role in Alzheimer's disease (AD) and different studies reported an age-associated dysregulation of the neuroimmune system. Consequently, we sought to characterize the pattern of microglial cell activation and astrogliosis in brain post mortem tissue of pathologically confirmed cases of early and late onset AD (EOAD and LOAD) and determine their relation to age. METHODS: Immunohistochemistry (CD68 and glial fibrillary acidic protein) with morphometric analysis of astroglial profiles in 36 cases of AD and 28 similarly aged controls. RESULTS: Both EOAD and LOAD groups had higher microglial scores in CA1, entorhinal and temporal cortices, and higher astroglial response in CA1, dentate gyrus, entorhinal and temporal cortices, compared to aged matched controls. Additionally, EOAD had higher microglial scores in subiculum, entorhinal and temporal subcortical white matter, and LOAD higher astrogliosis in CA2 region. CONCLUSIONS: Overall, we found that the neuroinflammatory pathological markers in late stage AD human tissue to have a similar pattern in both EOAD and LOAD, though the severity of the pathological markers in the younger group was higher. Understanding the age effect in AD will be important when testing modifying agents that act on the neuroinflammation.


Subject(s)
Alzheimer Disease/metabolism , Astrocytes/metabolism , Brain/metabolism , Gliosis/metabolism , Microglia/metabolism , Adult , Age Factors , Age of Onset , Aged , Aged, 80 and over , Alzheimer Disease/pathology , Antigens, CD/metabolism , Antigens, Differentiation, Myelomonocytic/metabolism , Astrocytes/pathology , Biomarkers/metabolism , Brain/pathology , Female , Glial Fibrillary Acidic Protein/metabolism , Gliosis/pathology , Humans , Immunohistochemistry , Male , Microglia/pathology , Middle Aged
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