ABSTRACT
In article is presented, the analysis of the data of the literature and own researches, concerning molecular-genetic mechanisms of antimycobacterial immunity infringements. The role of the factors defining features of a current and an outcome of a tubercular infection, namely of immunity system condition, feature of infecting microbic Mycobacterium tuberculosis strain biological properties (a genotype, a spectrum of drag sensitivity/resistance), influence of combined antitubercular chemotherapy is disc issed. The opinion is expressed that the given factors are necessary for taking into consideration for working out methodology the personalised preventive maintenance and correction of immunity infringements during pulmonary tuberculosis.
Subject(s)
Drug Resistance, Multiple, Bacterial , Immunotherapy , Mycobacterium tuberculosis/drug effects , Tuberculosis/immunology , Tuberculosis/therapy , Antitubercular Agents/therapeutic use , Cytokines/biosynthesis , Cytokines/immunology , Drug Therapy, Combination , Humans , Immunity, Cellular/genetics , Immunity, Cellular/immunology , Interleukin-12/immunology , Microbial Sensitivity Tests , Mycobacterium tuberculosis/genetics , Prognosis , Th1 Cells/immunology , Th1 Cells/metabolism , Tuberculosis/genetics , Tuberculosis/microbiologyABSTRACT
The data of modem literature and the results of original investigations on mechanisms of immunopathological alterations in tuberculosis infection are presented. The role of cellular and humoral parts of immunity in pathogenesis of pulmonary tuberculosis is discussed and cytokine-mediated mechanisms of disorders in a specific immune response are analysed.
Subject(s)
Immune System , Tuberculosis, Pulmonary , Apoptosis/immunology , B-Lymphocytes/immunology , Cytokines/immunology , Humans , Immune System/immunology , Immune System/pathology , T-Lymphocytes/immunology , Tuberculosis, Pulmonary/immunology , Tuberculosis, Pulmonary/pathologyABSTRACT
Peripheral blood lymphocytes were electron microscopically studied in patients with disseminated drug-sensitive and drug-resistant pulmonary tuberculosis before and during specific antituberculous chemotherapy. There was a tendency for an increase in the count of lymphocytes with membranous morphostructural changes during the therapy, which was more clearly defined in the drug-resistant type of the infectious process. It is suggested that antituberculous drugs have a damaging effect on the membrane of immunocompetent blood cells.
Subject(s)
Antitubercular Agents/adverse effects , Lymphocytes/drug effects , Lymphocytes/ultrastructure , Tuberculosis, Multidrug-Resistant/immunology , Tuberculosis, Pulmonary/immunology , Adult , Aged , Antitubercular Agents/administration & dosage , Female , Humans , Lymphocyte Count , Male , Microscopy, Electron , Middle Aged , Tuberculosis, Multidrug-Resistant/drug therapy , Tuberculosis, Pulmonary/drug therapyABSTRACT
The characteristics of the functional status of peripheral blood monocytes/macrophages in patients with Ixodes tick-borne acute borreliosis accompanied by opisthorchiasis invasion were studied. The study revealed a decrease in the phagocytic activity of monocytes and in the level of expression of cell receptors Fcgamma with the expression of cell receptors C3beta being normal.
Subject(s)
Borrelia burgdorferi , Lyme Disease/complications , Lyme Disease/immunology , Monocytes/immunology , Opisthorchiasis/complications , Opisthorchis , Animals , Chronic Disease , Humans , Lyme Disease/physiopathology , Macrophages/immunology , Macrophages/pathology , Monocytes/pathology , Phagocytosis/immunology , Receptors, IgG/biosynthesisABSTRACT
The characteristics of the functional activity of phagocyte blood cells in patients with destructive pulmonary tuberculosis caused by medicinal sensitive and medicinal resistant infective agents were studied. In the process of pulmonary tuberculosis, irrespective of the medicinal sensitivity of infective agents before and during treatment, a decrease in the phagocytic activity of neutrophil granulocytes and the level of the expression of Fcgamma- and C3b-receptors on monocytes with a simultaneous increase in the spontaneous production of oxygen metabolites in neutophils and a rise in the adsorptive capacity of monocytic cells were observed.
Subject(s)
Neutrophils/immunology , Tuberculosis, Pulmonary/immunology , Adult , Antitubercular Agents/therapeutic use , Blood Cells , Female , Humans , Male , Middle Aged , Monocytes/metabolism , Neutrophils/metabolism , Oxygen/metabolism , Phagocytosis , Receptors, Complement 3b/metabolism , Receptors, IgG/metabolism , Tuberculosis, Pulmonary/drug therapyABSTRACT
The authors studied functional characteristics of mononuclear leucocytes in patients with drug-sensitive and drug-resistant pulmonary tuberculosis. The study found decrease of the number of C3b- and Fcchi-receptorpositive monocytes and increase of their saturation activity in both categories of pulmonary tuberculosis before and after antituberculous therapy. The study revealed increase of interferons alpha and chi production and, at the same time, decrease of tumor necrosis factor alpha, which was more prominent in cases of drug-sensitive tuberculosis, and depression of interleukin 2 secretion, more prominent during chemotherapy in cases of drug-resistant tuberculosis.
Subject(s)
Leukocytes, Mononuclear/metabolism , Tuberculosis, Multidrug-Resistant/blood , Tuberculosis, Pulmonary/blood , Adult , Antitubercular Agents/therapeutic use , Biomarkers/blood , Female , Humans , Interferon-alpha/metabolism , Interleukin-2/metabolism , Male , Middle Aged , Receptors, Complement 3b/metabolism , Receptors, Fc/metabolism , Tuberculosis, Pulmonary/drug therapyABSTRACT
Tick-borne encephalitis is accompanied by dysregulation of cytokine production of mononuclear leucocytes of peripheral blood. The changes of production of IFN-r by mononuclear leucocytes of peripheral blood positively correlated with a degree of activity of pathological process and intensity of clinical symptoms of neuroninfection. On the contrary, secretion of IL-12 mononuclears directly correlated with virus persistence in the organism and does not depend on clinical presentation of the disease.
Subject(s)
Encephalitis, Tick-Borne , Interferon-gamma/biosynthesis , Interleukin-12/biosynthesis , Leukocytes, Mononuclear/immunology , Leukocytes, Mononuclear/metabolism , Adolescent , Adult , Encephalitis, Tick-Borne/blood , Encephalitis, Tick-Borne/immunology , Encephalitis, Tick-Borne/physiopathology , Female , Humans , Immunoglobulin G/immunology , Immunoglobulin M/immunology , Interferon-gamma/blood , Interferon-gamma/immunology , Interleukin-12/blood , Interleukin-12/immunology , Male , Middle AgedABSTRACT
An appreciable increase in the number of aberrant lymphocytes was detected in the peripheral blood of patients with febrile form of tick-borne encephalitis (TBE). This increase peaked during week 2 of the infectious process and was paralleled by a decrease in the count of natural killer cells. By the end of the acute period of neuroinfection the number of cells with structural chromosome aberrations decreased, but still surpassed the control (donor blood). Genetically unstable cells were eliminated predominantly at the expense of lymphocytes with chromatid disorders, while the count of CD16-expressing lymphocytes increased. The intensity of reparative DNA synthesis was suppressed during the entire acute period of tick-borne encephalitis.
Subject(s)
Chromosome Aberrations , Encephalitis, Tick-Borne/blood , Encephalitis, Tick-Borne/genetics , Lymphocytes/ultrastructure , Adult , DNA/biosynthesis , DNA Repair , Encephalitis Viruses, Tick-Borne/pathogenicity , Female , Fever/virology , Humans , Lymphocytes/blood , Lymphocytes/immunology , Male , Middle Aged , Receptors, IgG/analysisABSTRACT
We studied metabolic and functional activity of peripheral blood phagocytes from patients with febrile tick-borne encephalitis. Phagocytic activity of neutrophils determining nonspecific resistance of the organism progressively increased, while metabolic activity decreased. Secretion of nitric oxide by peripheral blood mononuclear phagocytes was suppressed, while phagocytic functions of these cells remained unchanged.
Subject(s)
Encephalitis, Tick-Borne/blood , Monocytes/physiology , Neutrophils/physiology , Acute Disease , Adult , Female , Humans , Male , Middle Aged , Monocytes/metabolism , Neutrophils/metabolism , Nitric Oxide/blood , Nitric Oxide/metabolismABSTRACT
Study of the surface phenotype, structure of plasma membrane, and activity of DNA repair system in peripheral blood lymphocytes from patients with different clinical forms of tick-borne encephalitis associated with long persistence of the virus revealed a pronounced imbalance between lymphocyte subpopulations, increased microviscosity of the plasma membrane lipid phase, and decreased activity of the DNA repair system in lymphocytes.
