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Ukr Biokhim Zh (1999) ; 74(4): 97-101, 2002.
Article in Ukrainian | MEDLINE | ID: mdl-14964869

ABSTRACT

The possibility of using different lectins for the determination of level of specific antibodies to leukosis virus virus in bovine blood serum whit the help of the surface plasmon resonance was investigated. The modification of transducer surface with lectins provides for immobilisation of glycosilated proteins of leukaemia virus. It allows to make selective determination of antibodies to glicosilated protein, the quantity of which more high in blood serum of ill animals in the comparison with the immunised ones. Moreover, the treatment of surface with FGA-P and PLA lectins increases the sensitivity of immune sensor to the specific antibodies in the comparison with the non-treated one.


Subject(s)
Antibodies, Viral/analysis , Biosensing Techniques , Enzootic Bovine Leukosis/blood , Glycoproteins/immunology , Lectins/metabolism , Leukemia Virus, Bovine/immunology , Animals , Antibodies, Viral/immunology , Cattle , Enzootic Bovine Leukosis/immunology , Immunodiffusion/methods , Immunodiffusion/veterinary , Surface Plasmon Resonance/methods , Surface Plasmon Resonance/veterinary , Viral Proteins/immunology
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