[Effects of cerebrospinal fluid preparation from male and female patients with opiate dependence on rat open-field behavior]. / Vliianie preparata likvora patsientov s opiinoi zavisimost'iu muzhskogo i zhenskogo pola na povedenie krys-retsipientov v teste otkrytogo polia.

The effects of the intranasal administration of preparations made from the cerebrospinal fluid of male and female opiate users on the open-field rat behavior were studied. Behavioral differences were demonstrated in the effects of preparations from female and male cerebrospinal fluid. The administration of the "male" preparation produced a significant decrease in the locomotor activity and increase in the immobilization time and grooming duration, while the "female" preparation had the opposite effects. These differences may result from different content of endogenous and exogenous opiates and dopamine (and its metabolites) in the cerebrospinal fluid of male and female opiate users.

[Effect of fractions of the cerebrospinal fluid from patients with drug dependence on basic electrophysiological characteristics of the rat olfactory cortex slices]. / Issledovanie éffektov otdel'nykh fraktsii likvora bol'nykh narkoticheskoi zavisimost'iu na bazisnye élektrofiziologicheskie kharakteristiki perezhivaiushchikh srezov kory mozga krys.

Heroin addicts at the initial stage of abstinence syndrome were subjected to detoxication by liquorosorption technique. The fractions of their cerebrospinal fluid obtained by the thin layer chromatography technique were analyzed. The substances extracted from the cerebrospinal fluid of drug addicts, presumably peptides, negatively affected the conductive function and synaptic transmission in surviving slices of the olfactory cortex of rats. The conclusion was drawn about a possibility of application of surviving rat brain slices as test object for estimation of the extent of purification of the cerebrospinal fluid from toxic endogenous substances after the liquorosorption.

[Role of the adenylate cyclase system in utilization of succinate by the frog gastric mucosa]. / Rol' adenilattsiklaznoi sistemy v utilizatsii suktsinata slizistoi obolochkoi zheludka liagushki.

Interrelationship between the adenylate cyclase system state and utilization of succinate as a substrate of oxidation in mechanisms of HCl secretion by isolated gastric mucosa of frog Rana esculenta was studied. The inhibition of the protein synthesis by cycloheximide rendered gastric mucosa insensitive to the activation of HCl secretion by histamine and succinate. A similar effect was exerted by cimetidine, a blocking agent for H2-receptors. The blockade could be prevented with dibutyryl-cAMP. The seasonal dependence of activation of HCl secretion by specific and unspecific modifiers was discovered. The data obtained show the necessity of preliminary activation of adenylate cyclase and accumulation of limiting content of cAMP for the utilization of succinate in reactions of generation and transport of H+ by acid-producing cells of isolated gastric mucosa in frog.

[Trigger function of cyclic AMP in the initiation of HCl secretion by the frog gastric mucosa]. / Triggernaia funktsiia tsiklicheskogo AMF v initsiatsii sekretsii HCl slizistoi obolochkoi zheludka liagushki.

Histamine (0.3 mM) caused a rapid but transient increase in cAMP level prior to a rise in HCl secretion in the isolated gastric mucosa of frog Rana esculenta. H2-receptor blocking agent cimetidine (0.01 mM) suppressed HCl secretion which had been preceded by a decrease in cAMP level. No statistically significant changes in cGMP content were found. Dibutyryl cAMP imitated the effect of histamine. In frogs captured in spring, with a high level of HCl secretion and high content of cAMP, histamine exerted no stimulating effect. It seems to be the cAMP and not cGMP that acts either as a trigger in activation of HCl secretion or as a stabilizer of induced secretion.