[ApoE gene polymorphism as a predictor of therapeutic response to treatment acetylcholinesterase inhibitor for elderly patients with Alzheimer's and vascular dementia types].

UNLABELLED: Effectiveness of donepezil hydrochloride treatment for elderly patients with Alzheimer's and vascular dementia types depending on the ApoE genotype. THE AIM: To study the effectiveness of donepezil hydrochloride (Almer "Actavis") for elderly patients with Alzheimer's and vascular dementia types depending on the ApoE genotype. METHODS AND SUBJECTS: The 3-month clinical study included 38 elderly patients (mean age- (71.03 ± 1.20) years) with dementia. All patients have undergone clinical, neurological, laboratory, instrumental (electrocardiography, CT/MRI brain), neuropsychological examination, ApoE genotype determination RESULTS: Identified certain characteristics of response to treatment of donepezil hydrochloride (acetylcholinesterase inhibitor) with dementia according to ApoE genotype. The effectiveness of donepezil hydrochloride was more pronounced in patients ε4 allele carriers compared with ε3/ε3 group the results of the scale parameter ADAScog, describing episodic memory and complex types of praxis. CONCLUSIONS: The effectiveness of the treatment of donepezil hydrochloride for elderly patients with dementia largely be associated with ApoE genotype available that makes it worthwhile carrying determine ApoE 2, 3, 4 allele in patients with cognitive deficits before starting pharmacotherapy.

[The activity of gas metabolism, thermoregulation, and antioxidant enzymes in aging C57Bl/6 mice].

The distribution type and correlative links between physiological and biochemical indices characterizing functional condition of the systems of gaseous exchange (V(O2) and V(CO2)), thermoregulation (body temperature and coefficient of thermoconductivity) and antioxidant defense have been studied in 62 young (3-5 mo.) and 58 old (23-26 mo.) male C57Bl/ 6 mice. The coefficients of variation differed significantly depending on the variable but not the age-group. Mean values of V(O2) and V(CO2), body temperature and thermoconductivity, but not activities of the antioxidant enzymes, declined in aging. Moreover, the activities of catalase, glutathione-peroxidase and glutathionereductase, i.e. enzymes involved in regulation of hydrogen peroxide level, increased in aging. The correlations between V(O2) and V(CO2), V(O2) and body temperature or V(O2) and the liver pH, as well as between the antioxidant enzyme activities exhibited little age-changes. However, three-dimensional non-linear models revealed significant age-changes in relations between the studied variables.

[Increase in T-cell level in the peripheral blood of women with fractures in anamnesis during postmenopause period].

44 postmenopausal patients (45-76 years of old) have been observed. Interrelationship between composition and functional activity of peripheral blood cells, TNF-alpha level in the serum and bone density in postmenopausal women has been analyzed. It was established that the increase in neutrophil activity in women with osteoporosis, the high level of immune complexes are accompanied by the high level of IgG and autoantibodies to DNA. These results allow suggesting the presence of chronic inflammatory or immune processes taken place in postmenopausal women with osteoporosis. Increase in CD3+ cells percent was established in women with fracture in anamnesis. Obtained results allow suggesting the possibility that T-cells may play a role in the occurrence of fractures.

[Role of genetic factors in the development of osteoporosis].

Osteoporosis which often accompanies the normal aging and a number of pathological conditions is closely related to bone remodeling disturbances. This process is provided mainly by the genesis of osteoblasts and osteoclasts, their activities and interactions. All the above in turn is dependent on numerous systemic and local regulatory factors such as hormones, nutrients, minerals, vitamins, cytokines, their receptors etc. Recently the crucial role of genetic polymorphism of many receptors in bone remodeling, bone loss and susceptibility to osteoporosis development has been shown. This review is devoted to the genetic background of interrelationships among the hormones, cytokines, vitamins and their receptors and discussion of possible sources of discrepancies in data obtained by many authors who studied populations like genetic, diet, life-styles and environmental influences.

[Correlation between gaseous exchange rate, body temperature, and mitochondrial protein content in the liver of mice]. / Koreliatsiina zalezhnist' mizh hazoobminom, temperaturoiu tila ta vmistom mitokhondrial'noho bilka pechinki myshei.

Correlative and regressive relations between the gaseous exchange, thermoregulation and mitochondrial protein content were analyzed by two- and three-dimensional statistics in mice. It has been shown that the pair wise linear methods of analysis did not reveal any significant correlation between the parameters under exploration. However, it became evident at three-dimensional and non-linear plotting for which the coefficients of multivariable correlation reached and even exceeded 0.7-0.8. The calculations based on partial differentiation of the multivariable regression equations allow to conclude that at certain values of VO2, VCO2 and body temperature negative relations between the systems of gaseous exchange and thermoregulation become dominating.

[Role of monoaminergic system in the mechanisms of developing a dependence on morphine]. / Rol' monoaminerhichnykh system u mekhanizmi rozvytku zalezhnosti vid morfinu.

The influence of morphine chronic administration on the neuromediators contents in various structures of brain has been studied. It has been shown that the development of physical dependence on the morphine and abstinence syndrome is accompanied by reduction of activity of serototinergic system, which is manifested in decrease of serotonin and its precursor tryptophane levels. Adrenergic and dophaminergic systems do not react to the development and abstinence by changes of the contents of monoamines that testifies of interaction of opioid and neuromediator systems disturbance.

[The characteristics of the development of the immune function in the transplanted spleen of newborn mouse pups in recipients of different ages. 2. The effect of the thymus on the functional development of the transplant]. / Osoblyvosti rozvytku imunnoï funktsiï peresadzhenoï selezinky navonarodzhenykh mysheniat retsypiientam riznoho viku. 2. Vplyv tymusa na funktsional'nyi rozvytok transplantata.

The effect of the neonatal thymus grafting or "Thymostimulin" administration on the cellularity, cell composition, immune, response to SRBC and proliferative activity of T- and B-cells in vitro were determined in neonatal spleen grafted CBA/Ca female mice of different ages. Analysis of the thymus graft effect on the T- and B-cells content in the spleen transplant from the adult and old recipients demonstrated no differences. The neonatal thymus grafting led to the essential increase of the immune response, spleen cellularity and to the diametrically opposed changing from negative to positive of the sign of the correlation coefficient between the T-cells content and the cellularity of the neonatal spleen in the old recipients. The similar effect of the neonatal thymus grafting was revealed in respect of correlative connection between content of the T-cells and PFCs in spleen grafted to the old recipients too. The "Thymostimulin" injection led only to the increase of the spleen transplant cellularity. These results suggests that the young thymic microenvironment is essential for the normal T-cells differentiation and for its normal function in the neonatal spleen transplant.

[The characteristics of the development of the immune function in the transplanted spleen of newborn mouse pups in recipients of different ages. I. The effect of the cells in the stromal microenvironment of the transplant]. / Osoblyvosti rozvytku imunnoï funktsiï peresadzhenoï selezinky novonarodzhenykh mysheniat retsypiientam riznoho viku. I. Vplyv klityn stromal'noho mikrootochennia transplantata.

The age-related changes in the cell composition, immune response to SRBC and proliferative activity of T- and B-cells in vitro were determined in neonatal spleen grafted to CBA/Ca female mice of different ages. The dependence of the immune response upon the recipient's age was observed. This result indicate that "young" transplant stromal cells are incapable of immune response increase in the old recipients. Analysis of the T- and B-cells content in the spleen grafted to the recipients of different ages demonstrated no differences. The decrease of the T-cell proliferative activity and existence of the inverse correlative connection between content of the T-cells and PFCs in spleen grafted to the old recipients were demonstrated. Additional neonatal thymus transplantation is proposed as tools for correction of the age-related changes in the immune function.