ABSTRACT
Bioluminescence activity and ATP pool were investigated in the culture of psychrophilic bacteria Photobacterium phosphoreum collected-from the exponential and stationary growth phases, as well as immobilized in polyvinyl alcohol (PVA) cryogel. In liquid culture, ATP pool remained at an almost a constant level throughout the luminescence cycle (over 100 h). The ATP pool in the stationary-phase and PVA-immobilizedl cells remained constant throughout their incubation in the medium (over 200 h) and in 3% NaCl solution (over 100 h): Quantitative assessment of integral photon yield and ATP pool indicated that bioluminescence decay in growing or stationary cells was not caused by limitation by the energy substrates of the luciferase reaction. Kinetic and quantitative parameters of emission activity and ATP pool excluded the possibility of formation of the aldehyde substrate for luciferase via reduction of the relevant fatty acids in NADPH and ATP-dependent reductase reaction and its oxidation in the monooxygenase reaction. Our results indicate that the aliphatic aldehyde is not utilized in the process of light emission.
Subject(s)
Adenosine Triphosphate/metabolism , NADP/metabolism , Photobacterium/metabolism , Cells, Immobilized/metabolism , Oxidation-Reduction , Polyvinyl Alcohol/chemistryABSTRACT
The ability to synthesize antibiotics and exoproteases was studied in 13 species belonging to the Actinomadura genus. The antibiotic and proteolytic activities were found only in A. fulvescens INA-3321 and A. citrea INA-1849. Glycerol was shown to be the best carbon source for the growth and biosynthetic activity of A. fulvescens. A. citrea had a wider spectrum of carbon sources used for the growth and a higher proteolytic and antibiotic activity. A. citrea exerted the maximal biosynthetic activity and the best growth in a medium with starch and xylose. The effect of different nitrogen sources on the growth and biosynthetic activity of A. citrea was also studied.
