ABSTRACT
Se evaluó la transfusión intraperitoneal en ratas y perros con vía alternativa para la reposición de sangre. Se inyectaron eritrocitos marcados con 59Fe a 5 perros en un volumen equivalente al 50% de la masa eritrocitaria teórica de los animales. Otros 3 perros y 12 ratas fueron inyectados con volúmenes de eritrocitos proporcionales al 9% del volumen globular de los animales. La máxima incorporación de eritrocitos a la circulación sanguínea de las ratas fue de 96ñ7% a las 48h. El T 1/2 de sobrevida de las células fue de 29 días. La máxima actividad promedio en todos los perros fue de 91.2ñ6% a las 96 h post-transfusión. Se verificó destrucción al azar de los eritrocitos y el tiempo medio de sobrevida se estimó en 66 días. La transfusión se efectuó en unos 15 segundos en las ratas y entre 2 y 4 minutos en los perros. En la necropsia se observaron reacciones serosas mínimas. El procedimiento es recomendable por su rapidez, seguridad y sencillez
Subject(s)
Animals , Dogs , Rats , Blood Component Transfusion/methods , Iron Radioisotopes/administration & dosage , Erythrocyte Aging , Erythrocyte Count , Erythrocyte Volume , Evaluation Study , Injections, IntraperitonealABSTRACT
Se evaluó la transfusión intraperitoneal en ratas y perros con vía alternativa para la reposición de sangre. Se inyectaron eritrocitos marcados con 59Fe a 5 perros en un volumen equivalente al 50% de la masa eritrocitaria teórica de los animales. Otros 3 perros y 12 ratas fueron inyectados con volúmenes de eritrocitos proporcionales al 9% del volumen globular de los animales. La máxima incorporación de eritrocitos a la circulación sanguínea de las ratas fue de 96ñ7% a las 48h. El T 1/2 de sobrevida de las células fue de 29 días. La máxima actividad promedio en todos los perros fue de 91.2ñ6% a las 96 h post-transfusión. Se verificó destrucción al azar de los eritrocitos y el tiempo medio de sobrevida se estimó en 66 días. La transfusión se efectuó en unos 15 segundos en las ratas y entre 2 y 4 minutos en los perros. En la necropsia se observaron reacciones serosas mínimas. El procedimiento es recomendable por su rapidez, seguridad y sencillez (AU)
Subject(s)
Animals , Dogs , Rats , Iron Radioisotopes/administration & dosage , Blood Component Transfusion/methods , Injections, Intraperitoneal , Erythrocyte Count , Erythrocyte Volume , Erythrocyte Aging , Evaluation StudyABSTRACT
The intraperitoneal transfusion of red cells in rats and dogs was evaluated as an alternative route for blood restoration. Erythrocytes labeled with 59Fe, equivalent to about 50% of the estimated total red cell volume, were injected in the abdominal cavity of 5 dogs. Three other dogs and 12 rats were injected with a volume proportional to the 9%. The maximal incorporation of tagged red cells in the circulation of the rats was of 96 +/- 7% at 48 h. One half of the erythrocytes disappeared from the circulation 29 days later. Computing data from all dogs, the mean highest value, 91.2 +/- 6%, was reached at 96 h and although random destruction of the cells occurred, the estimated T1/2 of survival was about 66 days. The injection was completed in about 15 seconds in the rats and in 2 to 4 minutes in the dogs. At the necropsy only minor serosal reactions were observed in the peritoneal cavity of the animals. Intraperitoneal transfusion can be recommended as a rapid, safe and efficient procedure for blood supply.
Subject(s)
Blood Component Transfusion/methods , Erythrocytes/diagnostic imaging , Animals , Dogs , Erythrocyte Aging , Erythrocyte Count , Erythrocyte Volume , Evaluation Studies as Topic , Injections, Intraperitoneal , Iron Radioisotopes , Radionuclide Imaging , RatsABSTRACT
The intraperitoneal transfusion of red cells in rats and dogs was evaluated as an alternative route for blood restoration. Erythrocytes labeled with 59Fe, equivalent to about 50
of the estimated total red cell volume, were injected in the abdominal cavity of 5 dogs. Three other dogs and 12 rats were injected with a volume proportional to the 9
. The maximal incorporation of tagged red cells in the circulation of the rats was of 96 +/- 7
at 48 h. One half of the erythrocytes disappeared from the circulation 29 days later. Computing data from all dogs, the mean highest value, 91.2 +/- 6
, was reached at 96 h and although random destruction of the cells occurred, the estimated T1/2 of survival was about 66 days. The injection was completed in about 15 seconds in the rats and in 2 to 4 minutes in the dogs. At the necropsy only minor serosal reactions were observed in the peritoneal cavity of the animals. Intraperitoneal transfusion can be recommended as a rapid, safe and efficient procedure for blood supply.
ABSTRACT
The intraperitoneal transfusion of red cells in rats and dogs was evaluated as an alternative route for blood restoration. Erythrocytes labeled with 59Fe, equivalent to about 50
of the estimated total red cell volume, were injected in the abdominal cavity of 5 dogs. Three other dogs and 12 rats were injected with a volume proportional to the 9
. The maximal incorporation of tagged red cells in the circulation of the rats was of 96 +/- 7
at 48 h. One half of the erythrocytes disappeared from the circulation 29 days later. Computing data from all dogs, the mean highest value, 91.2 +/- 6
, was reached at 96 h and although random destruction of the cells occurred, the estimated T1/2 of survival was about 66 days. The injection was completed in about 15 seconds in the rats and in 2 to 4 minutes in the dogs. At the necropsy only minor serosal reactions were observed in the peritoneal cavity of the animals. Intraperitoneal transfusion can be recommended as a rapid, safe and efficient procedure for blood supply.