1.
Brain Res
; 326(2): 384-7, 1985 Feb 11.
Article
in English
| MEDLINE
| ID: mdl-3971164
ABSTRACT
Rat cerebral cortex slices superfused for 5 min in sodium-free medium after 30-min stabilization and 25-min superfusion periods in sodium-containing medium displayed a well-preserved tissue structure and mostly intact neurons and glial cells. Electron-microscopic morphometry showed the number of presynaptic terminals containing few (less than or equal to 10) vesicles to increase 3-fold after the 5-min depletion of sodium. The 90-min sodium depletion resulted in increased swelling and an additional decrease in synaptic vesicles. The results show that from a morphological point of view the substitution of sodium by choline with brain slice techniques is an eminently suitable method for studies on synaptic neurochemistry.
Subject(s)
Cerebral Cortex/physiology , Sodium/physiology , Synaptic Vesicles/physiology , Animals , Calcium/metabolism , Cerebral Cortex/ultrastructure , Choline/pharmacology , Exocytosis , In Vitro Techniques , Microscopy, Electron , Rats , Rats, Inbred Strains , Synaptic Vesicles/ultrastructure
2.
Acta Physiol Scand
; 108(3): 313-5, 1980 Mar.
Article
in English
| MEDLINE
| ID: mdl-7376925