ABSTRACT
The ultrasensitive detection of 2,4,6-trinitrotoluene (TNT) was accomplished on the basis of sandwich-type TNT immunoassay combined with electrogenerated chemiluminescence (ECL) technology. Biotinylated anti-TNT species were attached to the surface of 1-microm diameter streptavidin-coated magnetic beads (MB) and 10-microm diameter avidin-coated polystyrene microspheres/beads (PSB) pre-loaded with ECL labels ( approximately 7 billion hydrophobic ruthenium(II) tris(2,2'-bipyridine) (Ru(II)) molecules per bead) to form anti-TNT<-->MB and anti-TNT<-->PSB(Ru(II)) conjugates, respectively. Sandwich-type PSB(Ru(II))<-->anti-TNTSubject(s)
Luminescence
, Soil/analysis
, Trinitrotoluene/analysis
, Water/chemistry
, Acetonitriles/chemistry
, Electrochemistry
, Immunoassay
, Magnetics
, Microspheres
, Polystyrenes/chemistry
, Propylamines/chemistry
, Sensitivity and Specificity
, Trinitrotoluene/chemistry