ABSTRACT
Social insects depend on their nests for protection against predation and abiotic threats. Accordingly, the chemical compounds present in the material wasps use to build their nests can both facilitate communication and repel predators. It is herein hypothesized that different wasp species build their nests with different structure and substrate materials and that such materials consist of chemical compounds related to unique wasp behavior and outside temperature variation. To test this hypothesis, nests were collected from three species of social wasps, the samples of which were subjected to temperature variation under laboratory conditions. The compounds present in the substrate were analyzed by gas chromatography coupled to mass spectrometry. Chemical compounds identified in the nest material of the three species responded differently to temperature variation. Chemical compounds from Polybia nests were altered significantly when subjected to temperature variation, whereas the nests of Polistes versicolor did not significantly change in relation to the control. The differences found between Polistes and Polybia nests may be related to genetic factors, but also to the type of nest they construct. It is possible that divergent evolutionary strategies for maintaining colony temperature, as a function of the chemical composition of the nests, may have appeared between wasps that have open and closed nests. In relatively small colonies, nest substrate is more resistant to temperature variation because it is composed of a greater diversity of elements and thus capable of holding heavier, longer carbon chains. Our results suggest that chemical compounds in the nest material of the three wasp species analysed responded differently to fluctuating ambient temperatures and that such variation could result from the biochemical differences of unique wasp species or from thermoregulation strategies of colonies.
Subject(s)
Body Temperature Regulation , Hydrocarbons/analysis , Nesting Behavior , Wasps/physiology , Animals , TemperatureABSTRACT
Most flies of forensic importance are in two superfamilies, the Muscoidea and the Oestroidea, with similar life stages including the puparium. Upon completion of metamorphosis the adult fly emerges from the puparium, leaving behind an exuvia that is of potential significance in forensic investigation. The empty puparium is a durable piece of entomological evidence lasting several years. Through the study of chemical compounds, specifically the hydrocarbons of these puparia, it is possible to identify the species, in addition to how long they have been exposed to weathering and for this reason, these parameters can assist forensic entomologists in estimating long-term postmortem interval (minPMI). In corpses that take a relatively longer time to decompose, insects may use the same corpses for several oviposition cycles. Therefore, the aim of this study was to develop a new method to determine the PMI based on chemical compounds of the puparia from different oviposition cycles of the fly Chrysomya megacephala. The chemical composition of 50 puparia from different cycles of oviposition were evaluated by Gas Chromatography-Mass Spectrometry (GC-MS). In total, 60 compounds were identified ranging from C18 to C34, 38 of those were common to all generations. Our results demonstrate that chemical profiles can be used to differentiate puparia collected from successive cycles, and therefore valuable in the estimation of minPMI.
