ABSTRACT
AIM: Study features of persistence of Burkholderia cepacia in mucoviscidosis patients. MATERIALS AND METHODS: In the period from 2008 to 2009, 56 B. cepacia strains isolated from children with mucoviscidosis were obtained. 114 medical histories of children with mucoviscidosis from various age groups were analyzed. The developed algorithm for identification and typing including phenotype and molecular biology methods was used to identify B. cepacia bacteria. Strain genotyping was carried out by RAPD-PCR with random oligonucleotide primer as well as pulse-electrophoresis. RESULTS: Persistence of associations ofmicroogranisms in 59.4% of cases was established to be the feature of persistent infection in mucoviscidosis. The feature of persistence of B. cepacia strains in patients with diagnosis ofmuco-viscidosis mixed form, severe course is persistence in association with Pseudomonas aeruginosa. B. cepacia bacteria that can persist in mucoviscidosis patients are characterized by resistance to many antibiotics. A prolonged (up to 1 year and 5 months) persistence of B. cepacia strains isolated from 1 patient was proven by using microflora monitoring of lower respiratory tract. CONCLUSION: B. cepacia bacteria may colonize lower respiratory tract of mucoviscidosis patients, persist for a long time and be transmitted between patients.
Subject(s)
Burkholderia Infections/microbiology , Burkholderia cepacia/pathogenicity , Cystic Fibrosis/microbiology , DNA, Bacterial/genetics , Pseudomonas Infections/microbiology , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/therapeutic use , Bacterial Typing Techniques , Burkholderia Infections/complications , Burkholderia Infections/drug therapy , Burkholderia cepacia/classification , Burkholderia cepacia/physiology , Child , Cystic Fibrosis/complications , DNA Fingerprinting , DNA Primers , DNA, Bacterial/analysis , Drug Resistance, Multiple, Bacterial , Humans , Phylogeny , Pseudomonas Infections/complications , Pseudomonas Infections/drug therapy , Pseudomonas aeruginosa/physiology , Random Amplified Polymorphic DNA Technique , Respiratory System/drug effects , Respiratory System/microbiology , Respiratory System/pathologyABSTRACT
AIM: To study microflora of lower respiratory tract of children from different age groups with cystic fibrosis during follow-up for determination of its variability and possible sources of infectious complications. MATERIALS AND METHODS: One hundred forty-one medical histories of patients from different age groups with cystic fibrosis living in various regions of Russian Federation were analyzed. Eighty-four children with cystic fibrosis living in Moscow and Moscow region treated as outpatients and inpatients were prospectively followed. For identification and characterization of microorganisms, microbiological, molecular biological, and statistical methods were used. RESULTS: It was demonstrated that chronic pseudomonas, staphylococcal or mixed infection was already diagnosed in 25% of children aged 1-4 years, and identified in 80% of patients to the age of 18 years. In two-thirds of cases association of microorganisms was identified, and in hospitalized patients these associations were comprised by 3-5 microorganisms in 60% of cases. Aside from main agents in associations (Pseudomonas aeruginosa and Staphylococcus aureus), representatives of Gram-negative nonfermentative microorganisms (Burkholderia cepacia, Stenotrophomonas maltophilia, Acinetobacter baumanii) were often identified that possibly determined by tropism of these species to lung tissue. CONCLUSION: Chronic mixed infection is characteristic for patients with cystic fibrosis. Identification of possible mechanisms of lung infection in patients with cystic fibrosis will allow to develop evidence-based system of prevention of infectious complications in these patients.
Subject(s)
Cystic Fibrosis/microbiology , Gram-Negative Bacteria/isolation & purification , Lung/microbiology , Respiratory Tract Infections/microbiology , Staphylococcus aureus/isolation & purification , Adolescent , Child , Child, Preschool , Chronic Disease , Cystic Fibrosis/complications , Humans , Infant , Infant, Newborn , Pseudomonas aeruginosa/isolation & purification , Respiratory Tract Infections/complications , Retrospective Studies , RussiaABSTRACT
In order to select bacteriocin producing strains the screening of microorganisms from clinical material was perfomed. The delayed antagonism method was used. 154 strains were screened, 93 (60%) cultures produced antagonistic substances. Micrococcus luteus NCTC, 2665, Candida albicans ATCC 885-653, Escherichihia coli 168/59, Pseudomonas aeruginosa 27/99, Klebsiella peumoniae 1954 were used as test-cultures, Pseudomonas aenruginosa and Staphylococcys aureus were the most frequent species producing antibacterial substances and their products were of high potency, with a wide spectrum of antimicrobial activity.
