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1.
Mem Inst Oswaldo Cruz ; 97(2): 265-8, 2002 Mar.
Article in English | MEDLINE | ID: mdl-12016455

ABSTRACT

One of the methods used for controlling cattle rabies in Brazil consists of vaccination. Sometimes, however, rabies occurs in cattle supposedly protected. Since rabies vaccine batches are officially controlled by tests performed on laboratory animals, it is questionable whether the minimal mandatory requirements really correspond to immunogenicity in the target species. We have analyzed the association among potencies of rabies vaccines tested by the NIH test, the contents and form (free-soluble or virus-attached) of rabies glycoprotein (G) in the vaccine batches, and the virus-neutralizing antibodies (VNA) titers elicited in cattle. No correlation was found between G contents in the vaccine batches and the NIH values, whatever the presentation of G. There was no correlation either between NIH values and VNA titers elicited in cattle. There was, however, a positive correlation (r = 0.8681; p = 0.0001) between the amounts of virion-attached G present in the vaccine batches and VNA elicited in cattle. This was not observed when the same analysis was performed with total-glycoprotein or free-soluble glycoprotein. The study demonstrated that NIH values can not predict the effect of the immunogen in cattle. On the other hand, the quantification of virus-attached rabies glycoprotein has a strong correlation with VNA elicited in cattle.


Subject(s)
Antibodies, Viral/immunology , Antigens, Viral , Glycoproteins/immunology , Rabies Vaccines/immunology , Viral Envelope Proteins/immunology , Animals , Antibodies, Viral/blood , Cattle , Cattle Diseases/immunology , Cattle Diseases/prevention & control , Enzyme-Linked Immunosorbent Assay , Glycoproteins/blood , Mice , Neutralization Tests , Rabies/prevention & control , Rabies/veterinary , Rabies Vaccines/standards , Viral Envelope Proteins/blood
2.
Mem. Inst. Oswaldo Cruz ; 97(2): 265-268, Mar. 2002. tab, graf
Article in English | LILACS, Sec. Est. Saúde SP | ID: lil-326286

ABSTRACT

One of the methods used for controlling cattle rabies in Brazil consists of vaccination. Sometimes, however, rabies occurs in cattle supposedly protected. Since rabies vaccine batches are officially controlled by tests performed on laboratory animals, it is questionable whether the minimal mandatory requirements really correspond to immunogenicity in the target species. We have analyzed the association among potencies of rabies vaccines tested by the NIH test, the contents and form (free-soluble or virus-attached) of rabies glycoprotein (G) in the vaccine batches, and the virus-neutralizing antibodies (VNA) titers elicited in cattle. No correlation was found between G contents in the vaccine batches and the NIH values, whatever the presentation of G. There was no correlation either between NIH values and VNA titers elicited in cattle. There was, however, a positive correlation (r = 0.8681; p = 0.0001) between the amounts of virion-attached G present in the vaccine batches and VNA elicited in cattle. This was not observed when the same analysis was performed with total-glycoprotein or free-soluble glycoprotein. The study demonstrated that NIH values can not predict the effect of the immunogen in cattle. On the other hand, the quantification of virus-attached rabies glycoprotein has a strong correlation with VNA elicited in cattle


Subject(s)
Animals , Cattle , Mice , Rabies virus , Rabies Vaccines , Glycoproteins , Antibodies, Viral , Rabies , Enzyme-Linked Immunosorbent Assay , Neutralization Tests , Rabies Vaccines , Glycoproteins , Antibodies, Viral
3.
Biologicals ; 29(2): 67-73, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11580211

ABSTRACT

The susceptibility of the C6 rat glioma cell line (ATCC; CCL-107) to rabies virus was characterized. The kinetics of infection performed with a fixed and a wild strain (from an infected cow) of rabies virus was monitored by direct immunofluorescence. Fluorescent cytoplasmic bodies were readily observed by UV microscopy from 24 hours post-infection (hpi) onwards. The ability of C6 to produce rabies infective virion particles was confirmed by determining the viral titres present in the supernatants of infected cultures, by both BHK-21 cell infection and mice inoculation. C6 cells produced similar viral titres to those produced by BHK-21 for both strains used. In addition, the yield of rabies glycoprotein was assessed by ELISA. In general, BHK-21 and C6 cells infected either by PV or with the wild rabies strain produced similar amounts of rabies glycoprotein. At 96 hpi, however, when the glycoprotein production peaked, BHK-21 infected with the wild strain produced significantly higher amounts of glycoprotein than C6. Subsequently, the optimal conditions for isolation of wild rabies virus strains from C6 cells were established and these proved to be as sensitive as NA cells in detecting 10 wild rabies samples. Due to the high sensitivity exhibited, C6 rat glioma cells present a new and useful system for rabies virus investigation.


Subject(s)
Antigens, Viral , Rabies virus/isolation & purification , Rabies virus/physiology , Virus Cultivation/methods , Animals , Cattle , Cell Line , Cricetinae , Fluorescent Antibody Technique, Direct , Glioma , Glycoproteins/biosynthesis , Mice , Nucleocapsid/biosynthesis , Nucleocapsid Proteins , Rats , Tumor Cells, Cultured , Viral Envelope Proteins/biosynthesis , Virus Replication
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