Initial Results Obtained with the First TWIN VLBI Radio Telescope at the Geodetic Observatory Wettzell.

Geodetic Very Long Baseline Interferometry (VLBI) uses radio telescopes as sensor networks to determine Earth orientation parameters and baseline vectors between the telescopes. The TWIN Telescope Wettzell 1 (TTW1), the first of the new 13.2 m diameter telescope pair at the Geodetic Observatory Wettzell, Germany, is currently in its commissioning phase. The technology behind this radio telescope including the receiving system and the tri-band feed horn is depicted. Since VLBI telescopes must operate at least in pairs, the existing 20 m diameter Radio Telescope Wettzell (RTW) is used together with TTW1 for practical tests. In addition, selected long baseline setups are investigated. Correlation results portraying the data quality achieved during first initial experiments are discussed. Finally, the local 123 m baseline between the old RTW telescope and the new TTW1 is analyzed and compared with an existing high-precision local survey. Our initial results are very satisfactory for X-band group delays featuring a 3D distance agreement between VLBI data analysis and local ties of 1 to 2 mm in the majority of the experiments. However, S-band data, which suffer much from local radio interference due to WiFi and mobile communications, are about 10 times less precise than X-band data and require further analysis, but evidence is provided that S-band data are well-usable over long baselines where local radio interference patterns decorrelate.

Monitoring of experimental rat lung transplants by high-resolution flat-panel volumetric computer tomography (fpVCT).

OBJECTIVE: Noninvasive assessment of experimental lung transplants with high resolution would be favorable to exclude technical failure and to follow up graft outcome in the living animal. Here we describe a flat-panel Volumetric Computed Tomography (fpVCT) technique using a prototype scanner. METHODS: Lung transplantation was performed in allogeneic as well as in corresponding syngeneic rat strain combinations. At different time points post-transplantation, fpVCT was performed. RESULTS: Lung transplants can be visualized in the living rat with high-spatial resolution. FpVCT allows a detailed analysis of the lung and the bronchi. Infiltrates developing during rejection episodes can be diagnosed and follow-up studies can easily be performed. CONCLUSIONS: With fpVCT it is possible to control the technical success of the surgical procedure. Graft rejection can be visualized individually in the living animal noninvasively, which is highly advantageous for studying the pathogenesis of chronic rejection or to monitor new therapies.

Acute rejection of experimental lung allografts: characterization of intravascular mononuclear leukocytes.

Leukocytes interacting with endothelia of lung allografts probably play a seminal role in acute rejection, but have not been characterized before. Transplantation was performed in the Lewis to Lewis and in the Dark Agouti to Lewis rat strain combinations. DNA replication was detected in T-cells on day 2 after pulse-labelling in vivo with 5-bromo-2'-deoxyuridine (BrdU). On day 5, leukocytes were isolated by intensive perfusion the graft, subject to flow cytometry and to quantitative RT-PCR. About 34 million leukocytes accumulated in allograft vessels, but only 10 and 6 million cells in isografts and control lungs, respectively. During rejection, IFN-gamma, IL-1beta and IL-10 mRNA expression increased, IL-12 mRNA decreased, whereas IL-2, IL-6, TNF-alpha, and TGF-beta mRNA did not change. The phenotype of graft monocytes was partially activated and intravascular T-cells proliferated. In conclusion, during rejection, monocytes with unusual properties accumulate and T-lymphocytes are activated in lung allograft blood vessels.