ABSTRACT
We demonstrate the highest intensity - 300 TW laser by developing booster amplifying stage to the 50-TW-Ti:sapphire laser (HERCULES). To our knowledge this is the first multi-100TW-scale laser at 0.1 Hz repetition rate.
Subject(s)
Aluminum Oxide , Amplifiers, Electronic , Lasers , Lenses , Equipment Design , Equipment Failure AnalysisABSTRACT
Despite all the advances in nonlinear microscopy, all existing instruments are constrained to obtain images of one focal plane at a time. In this Letter we demonstrate a two-photon absorption fluorescence scanning microscope capable of imaging two focal planes simultaneously. This is accomplished by temporally demultiplexing the signal coming from two focal volumes at different sample depths. The scheme can be extended to three or more focal planes.
Subject(s)
Microscopy, Electron, Scanning/instrumentation , Microscopy, Fluorescence/methods , Optics and Photonics , Equipment Design , Image Processing, Computer-Assisted , Imaging, Three-Dimensional , Isoquinolines/chemistry , Isoquinolines/pharmacology , Microscopy, Electron, Scanning/methods , Microspheres , Photons , Spectrophotometry , Time FactorsABSTRACT
Spatiotemporal pulse shaping is characterized with two-dimensional Fourier transform spectral interferometry. A deformable-mirror-based bidimensional pulse shaper is used to create simple spatiotemporal structures on a femtosecond pulse, structures that are directly calculated from the measured spatiospectral phases and intensities.
ABSTRACT
We demonstrate the use of a simple tool to simultaneously visualize and characterize chromatic and spherical aberrations that are present in multiphoton microscopy. Using two-dimensional Fourier transform spectral interferometry, we measured these aberrations, deducing in a single shot spatiotemporal effects in high-numerical-aperture objectives.
Subject(s)
Algorithms , Artifacts , Image Enhancement/methods , Image Interpretation, Computer-Assisted/methods , Interferometry/methods , Microscopy, Fluorescence, Multiphoton/methods , Refractometry/methods , Fourier Analysis , Reproducibility of Results , Sensitivity and Specificity , Spectrum Analysis/methodsABSTRACT
By using the third-harmonic signal generated at an air-dielectric interface, we demonstrate a novel way of correcting wavefront aberrations induced by high-numerical-aperture optics. The third harmonic is used as the input physical parameter of a genetic algorithm working in closed loop with a 37-actuator deformable mirror. This method is simple and reliable and can be used to correct aberrations of tightly focused beams, a regime where other methods have limitations. Improvement of the third-harmonic signal generated with an f/1.2 parabolic mirror by 1 order of magnitude is demonstrated.
ABSTRACT
We generated a record peak intensity of 0.7 x 10(22) W/cm2 by focusing a 45-TW laser beam with an f/0.6 off-axis paraboloid. The aberrations of the paraboloid and the low-energy reference laser beam were measured and corrected, and a focal spot size of 0.8 microm was achieved. It is shown that the peak intensity can be increased to 1.0 x 10(22) W/cm2 by correction of the wave front of a 45-TW beam relative to the reference beam. The phase and amplitude measurement provides for an efficient full characterization of the focal field.
