ABSTRACT
Spontaneous and mitogen-stimulated proliferation of spleen lymphocytes was inhibited by coculturing the cells with murine bone marrow derived macrophages (BM-M phi). The inhibitory effect was found to be dependent on the maturation stage of BM-M phi reflected by the appearance of the phenotype BM 8 and on the number of BM-M phi added to the lymphocytes. The spontaneous proliferation was only inhibited by the addition of high numbers of BM-M phi of late maturation stage. The lipopolysaccharide (Lps) induced response was strongly suppressed by increasing proportions of BM-M phi of either cultivation stage. The suppressive effect on the concanavalin A (Con A)-induced proliferative activity increased with the maturation of the macrophages. Inhibition of prostanoid release with indomethacin had no effect. Blocking of nitric oxide synthesis partially reversed the inhibitory effect of macrophages mainly on the Con-A response. Addition of culture supernatant of BM-M phi to the lymphocytes did not mimic the effect of the macrophages themselves. BM-M phi only slightly inhibited the proliferation when lymphocyte-BM-M phi cell-to-cell contact was prevented. These results show that BM-M phi differently influence the spontaneous and mitogen-induced lymphoproliferation and that the inhibitory effect of BM-M phi on the lymphocyte proliferation is only partially mediated by secretory products of macrophages but apparently requires cell-to-cell contact between both cell types.
Subject(s)
Bone Marrow Cells , Cell Adhesion/immunology , Lymphocyte Activation/immunology , Macrophages/physiology , Nitric Oxide/physiology , Prostaglandins/physiology , Animals , Arginine/analogs & derivatives , Arginine/pharmacology , Cells, Cultured , Concanavalin A/pharmacology , Culture Media, Conditioned , Female , Indomethacin/pharmacology , Lipopolysaccharides/pharmacology , Macrophages/immunology , Mice , Mice, Inbred Strains , omega-N-MethylarginineABSTRACT
A significant dependence between different ionic strength and pH value of virus suspension on one hand and the haemagglutinin (HA) content as determined by single-radial-immunodiffusion (SRD) test on the other hand was observed after cleavage of influenza virus recombinant NIB-6 (H1N1) with sodium lauroyl sarcosinate (SLS). In contrast, no such relationship was found when the HA Of NIB-4 (H3N2) recombinant strain was determined.
