Re-emergence of porcine epidemic diarrhea virus in a piglet-producing farm in northwestern Germany in 2019.

BACKGROUND: Porcine epidemic diarrhea (PED) is a viral enteric disease of pigs. It affects all age classes of animals but lethality is mainly seen in suckling piglets. After its first appearance in England in 1971, Porcine epidemic diarrhea virus (PEDV) has spread worldwide. While sporadic outbreaks prevailed in Europe, the disease had high impact in Asia. Following particularly severe outbreaks in 2011, high impact cases were also reported in the United States and neighboring countries in 2013. Subsequently, outbreaks were also reported in several European countries including Germany. These outbreaks were less severe. This case report describes a recent case of PED re-emergence in Germany and the sequence analyses of the causative PEDV. CASE PRESENTATION: In spring 2019 5 years after re-introduction of PED into Central Europe, a piglet-producer in northwestern Germany experienced an outbreak that affected sows, their suckling piglets, and weaners. After initial confirmation of PEDV by real-time RT-PCR, fecal material and small intestine samples from affected pigs were subjected to metagenomic analyses employing next-generation sequencing. Phylogenetic analyses showed high identities among the PEDV sequences obtained from samples of different animals and a close relation to recent strains from Hungary and France. Compared to the PEDV strains analyzed in 2014, genetic drift could be confirmed. Changes were mainly observed in the spike protein encoding S gene segment. In addition, metagenomic analyses showed multiple Picobirnavirus reads in all investigated samples. CONCLUSION: This case report shows that PEDV is still circulating in Europe. The causative strains are moderately virulent and are still closely related to the so-called INDEL strains reported previously in Europe, including Germany. However, a genetic drift has taken place that can be seen in a novel cluster comprising strains from Germany, Hungary and France in 2019. Relevance and impact of the detected Picobirna sequences need further investigations.

[Feed refusal in fattening bulls because of maize silage contamination by Jimson weed (Datura stramonium)]. / Futterverweigerung bei Mastbullen durch eine Kontamination der Maissilage mit Gewöhnlichem Stechapfel (Datura stramonium).

A sudden decrease in feed intake (within 24 hours) without an impaired general condition occurred on a bull-rearing and -fattening farm in western Germany in December 2017. One day later, some of the bulls displayed signs of ruminal tympany (free gas bloat) and the reduction in feed intake became more severe. The maize silage was recognized as the cause of the observed symptoms, because clinical signs first appeared after taking the silage from a different localization within the bunker silo. Maize in this particular section was harvested from a separate area previously affected by wind damage (numerous bent maize plants), where no crop protection (herbicide application) was performed. Fruits and seeds of Jimson weed were identified in the incriminated maize silage samples. Following replacement of the contaminated parts of the maize silage with non-contaminated material and treatment of the clinically affected animals (anti-tympanic medication plus live yeast preparation), the feed intake returned to the normal level and the affected animals recovered within 6 days. The cause of the symptoms was probably the anti-nutritive properties of Datura stramonium that affect the motor function of the gastrointestinal tract.