A Quantitative Assessment of Trainers-Dolphins' Interactions in the Absence of Food Reward.

All the studies that have considered the motivation of the dolphins to interact with their trainers as a possible welfare indicator have been carried out in facilities where the trainer-dolphin interactions (TDIs) sessions were reinforced with food. Therefore, in these specific circumstances, it was difficult to separate the motivation of the dolphins interacting with the trainers from the food drive. The current study aims to assess the interaction between the trainers and the dolphins in the absence of food rewards. The research was carried out at The Dolphin Reef (Eilat, Israel), a facility where the interaction between the trainers and 14 bottlenose dolphins of different sex and age classes did not involve food rewards. A total of 531 TDIs were recorded, with dolphins participating in 94.5% of the sessions and an average of three dolphins per session. The dolphins participated in a higher number and more frequently in the TDIs when toys were provided by the trainers. Diel and seasonal differences were also observed, with the dolphins participating more during the morning sessions and the neutral season. The latency of response of the dolphins to the presence of the trainers on the platform or in the water, whether or not advertised by the trainers' signal ("call" or "no-call"), was very short (usually less than 1 min), and the dolphins often anticipated the beginning of the sessions by arriving at the trainers' location before or upon the caretakers' arrival (96% of the time). Individual differences in the participation in the TDIs were also recorded, which might be linked to both the dolphin's health/welfare status or their personality. The current study shows that the separation of the TDIs from the food reward allows for a better understanding of the willingness of dolphins under human care to interact with their trainers. In addition, the results presented in this paper show that such TDIs are an important part of these dolphins' lives, which suggests that these interactions might be an additional tool to improve the animals' social environment and monitor their welfare.

Changes in the Dog's and Cat's Behaviors, as Reported by the Owners, before and during the Lockdown in China.

Considering the effects that the COVID-19 pandemic had and still has on human psychological health, it is expected that it might also affect household dogs' and cats' welfare. The current study explores the behavioral changes in dogs and cats before (BL) and during the lockdown (DL), as reported by their owners in China. Besides demographic parameters, variables related to the daily management of dogs and cats were analyzed in relation to behavioral problems, stress-related behaviors, and anxiety-related behaviors before and during the lockdown. A total of 261 questionnaires were collected. In general, behavioral problems and stress-related behaviors in dogs (p < 0.001) and cats (p < 0.001) decreased DL compared to BL, while anxiety-related behaviors in cats did not show any differences between the two periods considered. On the other hand, anxiety-related behaviors were more frequent in dogs DL (36.3%) compared to BL (35%), which were associated with reduced frequency of play activities with the owners (p = 0.016) and altered sleeping habits (p < 0.01). During the lockdown, dogs' and cats' daily routines and management (feeding and sleeping habits, dogs' walks, dogs' and cats' play activities, litter box management, and cats' lifestyle) experienced changes, but they were not associated with any behavioral issues. On the other hand, the behavioral issues considered for dogs and cats were more frequent BL, which were influenced by the daily management of the pets. The current study showed how critical the attention the owners can provide to the pets could be, to improve their companion animals' welfare. Therefore, it is important to provide pet owners with behavioral management support both during particularly difficult periods such as a lockdown and during regular daily routines.

Chinese College Students' Attitudes towards Animal Welfare.

Understanding the attitude of stakeholders towards animals is critical for the development and improvement of animal welfare in a country. College students from veterinary, animal, and life sciences majors represent future key stakeholders that will interact with professionals from animal industries. Therefore, it is critical to understand these college students' attitudes towards animals and their knowledge about animal welfare. The present survey aimed to investigate Chinese college students' concerns towards different animal classes (i.e., pets, farm, laboratory, and wild animals) through the animal Sentient and Five Freedoms models. Chinese college students from different majors (i.e., related to animal sciences or not) scored very well in their attitude towards both the animal Sentient and Five Freedoms models, with differences depending on the animal class considered. Pets (dogs and cats) had better consideration for both animal Sentient and Five Freedoms models, followed by wild animals, while farm and laboratory animals were less considered. Veterinary science major students showed the strongest differences in attitudes depending on the animal classes considered compared to other majors. Furthermore, respondents showed better attitude scoring if they currently owned or had owned animals, had participated in animal welfare courses, or in laboratory work that involved animals. When compared to previous studies, our results suggest a general improvement of Chinese college students' attitudes towards animals.

Successful captive breeding of a Malayan pangolin population to the third filial generation.

Pangolins are threatened placental mammals distributed in Africa and Asia. Many efforts have been undertaken in the last century to maintain pangolins in captivity, but only a few of them succeeded in maintaining and keeping this species in a controlled environment. This study reports the first systematic breeding of the Critically Endangered Malayan pangolin (Manis javanica) in captivity. Our captive breeding approach successfully improved the reproductive rate for both wild and captive-born female pangolins. From 2016 to 2020, we had 33 wild pangolins and produced 49 captive-born offspring spanning three filial generations. The female offspring further bred 18 offspring, of which 14 (78%) were conceived during the first time of cohabitation with males, and four offspring were conceived during the second cohabitation event, suggesting that they may practice copulation-induced ovulation. We observed that captive-born female pangolins could reach sexual maturity at 7-9 months (n = 4), and male pangolins could mate and successfully fertilise females at nine months age (n = 1). We also observed a female pangolin conceiving on the eighth day after parturition (the fifth day after the death of its pup). Our captive pangolins had a female-biased sex ratio of 1:0.5 at birth, unlike other known captive-born mammals. Also, captive-born pangolins were generally more viable after successful weaning and had a similar gestation length (~185 days) to wild pangolins. Most importantly, we report the first self-sustaining captive population of Malayan pangolins, and this species has an efficient reproduction strategy. These advances provide more comprehensive information for people to understand pangolins, and have implications for conserving endangered Malayan pangolins and providing scientific guidance to the management of other pangolin species.

Biodiversity loss and COVID-19 pandemic: The role of bats in the origin and the spreading of the disease.

The loss of biodiversity in the ecosystems has created the general conditions that have favored and, in fact, made possible, the insurgence of the COVID-19 pandemic. A lot of factors have contributed to it: deforestation, changes in forest habitats, poorly regulated agricultural surfaces, mismanaged urban growth. They have altered the composition of wildlife communities, greatly increased the contacts of humans with wildlife, and altered niches that harbor pathogens, increasing their chances to come in contact with humans. Among the wildlife, bats have adapted easily to anthropized environments such as houses, barns, cultivated fields, orchards, where they found the suitable ecosystem to prosper. Bats are major hosts for αCoV and ßCoV: evolution has shaped their peculiar physiology and their immune system in a way that makes them resistant to viral pathogens that would instead successfully attack other species, including humans. In time, the coronaviruses that bats host as reservoirs have undergone recombination and other modifications that have increased their ability for inter-species transmission: one modification of particular importance has been the development of the ability to use ACE2 as a receptor in host cells. This particular development in CoVs has been responsible for the serious outbreaks in the last two decades, and for the present COVID-19 pandemic.

History of the COVID-19 pandemic: Origin, explosion, worldwide spreading.

The SARS-CoV-2 virus of the COVID-19 pandemic, that is presently devastating the entire world, had been active well before January of this year, when its pathogenic potential exploded full force in Wuhan. It had caused the onset of small disease outbreaks in China, and probably elsewhere as well, which failed to reach epidemic potential. The distant general origin of its zoonosis can be traced back to the ecosystem changes that have decreased biodiversity, greatly facilitating the contacts between humans and the animal reservoirs that carry pathogens, including SARS-CoV-2. These reservoirs are the bats. The transition between the limited outbreaks that had occurred through 2019 and the epidemic explosion of December-January was made possible by the great amplification of the general negative conditions that had caused the preceding small outbreaks. In the light of what we have now learned, the explosion was predictable, and could have happened wherever the conditions that had allowed it, could be duplicated. What could not have been predicted was the second transition, from epidemic to pandemic. Research has now revealed that the globalization of the infection appears to have been caused by a mutation in the spike protein of the SARS-CoV-2, that has dramatically increased its transmissibility.

COVID19: an announced pandemic.

A severe upper respiratory tract syndrome caused by the new coronavirus has now spread to the entire world as a highly contagious pandemic. The large scale explosion of the disease is conventionally traced back to January of this year in the Chinese province of Hubei, the wet markets of the principal city of Wuhan being assumed to have been the specific causative locus of the sudden explosion of the infection. A number of findings that are now coming to light show that this interpretation of the origin and history of the pandemic is overly simplified. A number of variants of the coronavirus would in principle have had the ability to initiate the pandemic well before January of this year. However, even if the COVID-19 had become, so to say, ready, conditions in the local environment would have had to prevail to induce the loss of the biodiversity's "dilution effect" that kept the virus under control, favoring its spillover from its bat reservoir to the human target. In the absence of these appropriate conditions only abortive attempts to initiate the pandemic could possibly occur: a number of them did indeed occur in China, and probably elsewhere as well. These conditions were unfortunately present at the wet marked in Wuhan at the end of last year.

Behavioral laterality in Yangtze finless porpoises (Neophocaena asiaeorientalis asiaeorientalis).

The Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis) is a critically endangered species with less than 1000 individuals expected to be left in the wild. While many studies have been conducted on laterality among several cetacean species, no studies investigating the Yangtze finless porpoise have been conducted. Using event sampling methods, several behaviors such as flipper-body touching, object touching, barrel-rolls, side swimming, and swimming direction were recorded from six captive porpoises (three males and three females). Analyses of 360 observations recorded over two months revealed that, at group level, porpoises showed laterality in swimming behaviors. Porpoises swam preferentially with their right pectoral fin upward and their left pectoral fin downward with a clockwise swimming direction and also displayed a consistent bias for a counterclockwise barrel-roll direction. No significant differences were reported for flipper use either during the interaction with conspecifics or with objects. The results from the current study provide novel insight into the cerebral asymmetry in a species previously ignored within the literature, thus improving our understanding on the extent of laterality in cetaceans and on the evolutionary history of hemispheric laterality for vertebrates in general.

Identification of Atypical Enteropathogenic Escherichia coli O98 from Golden Snub-Nosed Monkeys with Diarrhea in China.

Fecal samples (n = 76) were collected from 38 snub-nosed monkeys (Rhinopithecus roxellana) in Shennongjia National Nature Reserve (China) and examined for the presence of enteropathogenic Escherichia coli (EPEC). The 56 samples originated from 30 free-ranging monkeys on the reserve and 20 samples from 8 captive monkeys that were previously rescued and kept at the research center. Eight diarrhea samples were collected from four of the eight captive monkeys (two samples from each monkey), and two EPEC strains (2.6%) (95% confidence interval 0.3-9.2%) were isolated from two fecal samples from two diarrheic monkeys. Both strains belonged to serotype O98 and phylogenetic group D (TspE4C2+, ChuA+). The virulence gene detection identified these strains as an atypical EPEC (aEPEC) (bfpB - , stx1 - , and stx2-) with the subtype eae+, escV+, and intiminß+. These strains were highly sensitive to all the antibiotics tested. The lethal dose 50% of the two isolates in Kunming mice was 7.40 × 108 CFU/0.2 mL and 2.40 × 108 CFU/0.2 mL, respectively, indicating low virulence. Based on the report that this serotype had been isolated from some other non-human animals and humans with diarrhea, the first identification of aEPEC O98 strains and their drug resistance profile in R. roxellana is of ecological significance for disease control in this endangered species.