[The cytogenetic effect of cyclophosphane and the formation of active forms of oxygen in the bone marrow cells of C57Bl/6 and NZW mice]. / Tsitogeneticheskii éffekt tsiklofosfana i obrazovanie aktivnykh form kisloroda v kletkakh kostnogo mozga myshei linii C57Bl/6 i NZW.

Cytogenetic study conducted after a single intraperitoneal injection of cyclophosphane in doses of 10, 20, and 40 mg/kg showed in 1.5-2-month-old male mice that the level of bone marrow cells damaged by the mutagen was significantly higher in NZW animals than in C57Bl/6 animals. The ability to produce active oxygen forms in response to addition of opsonized zymosan and phorbol myristate acetate was also higher in bone marrow suspensions of NZW mice. The higher sensitivity of NZW animals to pro-oxidant and clastogenic effects may be related to the genetically determined decrease of antioxidant protection in NZW animals.

[Effect of dimethyl sulfoxide and dimethyl sulfone on a destructive process in the joints of mice with spontaneous arthritis]. / Vliianie dimetilsul'foksida i dimetilsul'fona na destruktivnyi protsess v sustavakh pri spontannom artrite u myshei.

The authors used the blind method for evaluation of the morphological picture of the joints and the level of circulating immune complexes to study the effect of prolonged oral administration of dimethyl sulfoxide (DMSO) and its main metabolite dimethyl sulfone on the development of spontaneous arthritis in 36 Mrl/Mn/lnr female mice. It was found that DMSO and dimethyl sulfone lessen the destructive changes in the joints, while DMSO also inhibits the manifestation of immune disorders, i. e. produces a "basal" effect on the course of spontaneous chronic arthritis in experimental animals.

[The effect of splenic factors on the course of the autoimmune process in New Zealand mice]. / Vliianie selezenochnykh faktorov na techenie autoimmunnogo protsessa u novozelandskikh myshei.

To exert an effect on the autoimmune process in 54 New Zealand mice aged 6 and 10 months, 0.2 ml of a solution obtained in perfusion of isolated pig's spleen (perfusate) was injected intravenously at intervals of 2--3 days (a total of 8 injections). The control group (43 mice) received injections of an isotonic buffer solution in the same regimen. After the end of perfusate administration the number of antibodies to DNA and the level of circulating immune complexes (IC) reduced markedly in the blood of the experimental mice as compared to these values in the control group. Kidney immunofluorescence demonstrated reduced number of IC deposits in the glomeruli of 6- and 10-month old mice of the experimental group. The perfusate contributes, probably, to intensified excretion of IC from the blood flow and kidneys of the experimental mice.

[Characteristics of memory in MRL/1 mice and the effect of thymic peptides]. / Osobennosti pamiati u myshei MRL/1 i vliianie na nee peptidov timusa.

By means of methods of active and passive getting rid of electrical-pain irritation we showed that in mice MRL/1--the model of rheumatoid arthritis (RA)--as compared with CBA (control) the process of forming a developed habit engram (DHE) was slowed down and its keeping was impaired. Thymic peptides (thymalin--0.2 mlg/mice intraperitoneally) suppressed the process of forming DHE irrespective of mice line and improved the process of its consolidation and keeping especially in mice MRL/1. Memory impairment in mice with genetical predisposition to the development of autoimmune process (MRL/1) is considered from view of the authors' developed hypothesis about thymus as an organ of antisystem of immune control of homeostasis and RA as an adaptation disease.

[Elimination of immune complex deposits from the kidneys of autoimmune NZB/N mice administered a xeno-spleen perfusate]. / Eliminatsiia otlozhenii immunnykh kompleksov iz pochek autoimmunnykh myshei NZB/N pri vvedenii perfuzata ksenoselezenki.

54 autoimmune NZB/N mice, 6 and 10 months of age, were intravenously injected 0.2 ml solution prepared during perfusion of the isolated sheep spleen with a buffer solution. Perfusion solution was injected 8 times with 2-3-day intervals. The control group of 43 mice received intravenously 0.2 ml of a buffer solution in the similar manner. After the treatment the levels of anti-DNA antibodies and circulating immune complexes were significantly decreased in the sera of mice which received the perfusion solution, as compared with the levels of control groups. Immunofluorescent studies showed a marked decrease in the number of glomerular immune complexes deposits in mice treated with perfusion solution. Six- and ten-month old mice exhibited a similar effect. The perfusion solution may be capable of eliminating the immune complexes from the blood and kidneys of autoimmune mice.

[Electron microscopic characteristics and polypeptide composition of the nuclear matrix of liver cells in mice with autoimmune disease]. / Elektronno-mikroskopicheskaia kharakteristika i polipeptidnyi sostav iadernogo matriksa kletok pecheni myshei s autoimmunnym zabolevaniam.

A study was made of the ultrastructure and polypeptide composition of liver cell nuclear matrix of F1NZB/NZW hybrid mice imitating human systemic Lupus erythematosus. Electron microscopy reveals enlargement in fibrous lamina diameter and increase in pore complex density up to the age of 8-9 months. In the terminal stages of the disease (12-13 months of age) a gradual attenuation of the intranuclear matrix and disappearance of pore complexes is observed along with a segregation and subsequent fragmentation of residual nucleoli which results eventually in the general degradation of the nuclear matrix. 30-35 polypeptide bands with molecular weight from 200 to 10 kD are revealed in polyacrylamide gel electrophoresis of the liver cell nuclear matrix of hybrid mice. Several protein bands in the high molecular weight region of 200-150 kD are strongly enhanced, and a triplet with molecular weight 70-60 kD is distinctly visible. The results obtained are interpreted as an indication of a protecting cellular reaction against antinuclear autoantibodies in the earlier stages, and a degradation of the nuclear matrix in terminal stages of the disease. It is supposed that the electron microscopic and electrophoretic patterns of the nuclear matrix indicate an accumulation of collagenous proteins.

[Autoantibodies to the thymus and skin epithelium in NZB/N mice and (NZBxNZW)F1 hybrids]. / Autoantitela k épitelial'noi tkani timusa i kozhi u myshei linii NZB/N i u gibridov (NZBxNZW)F1.

Antibodies reacting with thymus and skin epithelial cells were revealed by indirect immunofluorescence in sera of NZB/N mice and (NZB X NZW)F1 hybrids (B/W) 1-2 and 4-5 months of age. Similar antibodies were not found in sera of BALB/c mice. The inhibition experiments with DNA have shown that antibodies reacting with the thymus and skin epithelium differ from those reacting with the cellular nucleus. Positive reactions with the epithelium were obtained in all thymus and skin tissue samples of humans, guinea-pigs and NZB/N, B/W and BALB/c mice, including autologous tissues of NZB/N and B/W mice. Thus, antibodies reacting with thymus and skin epithelial tissues belong to autoantibodies. These autoantibodies are revealed during the first month of life before the onset of autoimmune processes. The role of these autoantibodies in the damage of thymus epithelium and the development of immunoregulatory disturbances, typical of autoimmune processes, needs further study.

[DNAse II from phagocytes in the study of the pathogenesis of systemic lupus erythematosus]. / DNKaza II kletok fagotsitiruiushchego riada v izuchenii patogeneza sistemnoi krasnoi volchanki.

Activity of acid DNAase (DNAase II) was shown to decrease in neutrophils from peripheric blood of patients with systemic lupus erythematosus as well as in macrophages of liver tissue from mice of the F1(NZB/w) strain during development of the lupus syndrome. Inhibition of the DNAase II activity in phagocytic cells might be among the reasons of accumulation of extracellular highly polymeric DNA in blood of the patients with systemic lupus erythematosus and to play a definite role in pathogenesis of the disease.

[Morphogenetic characteristics of the mammary glands of autoimmune F1 mice (NZB x NZW)]. / Osobennosti morfogeneza molochnykh zhelez autoimmunnykh myshei F1 (NZB x NZW).

The morphogenesis of mammary glands was studied in the normal and autoimmune F1(NZW X NZB) mice. In the lactation cycle of the autoimmune mice the normal course of structural-functional rearrangements of parenchyma and stroma in the developing and involuting mammary glands was disturbed. A conclusion has been reached that the modification of stromal elements, first of all involved in the autoimmune disease, is the leading link in the abnormal development of mammary glands.

[Genetic aspects of the reactions of humoral immunity to collagen in man and animals. II. Modifications of the autoimmune status to collagen type I in NZB x NZW (F1) mice by sex hormones during ontogeny]. / Geneticheskie aspekty reaktsii gumoral'nogo immuniteta k kollagenu u cheloveka i zhivotnykh. Soobshchenie II. Modifikatsii autoimmunnogo statusa k kollagenu tipa I u myshei NZB x NZW (F1) polovymi gormonami v ontogeneze.

The sex hormones, estradiol and testosterone, are able to modulate the status of spontaneous reactions of humoral immunity to type I collagen in ontogenesis of NZB x NZW (F1) females. Administration of estradiol to puber and unpuber females leads to a significant increase in the reactivity levels. The autoimmune status to type I collagen in NZB x NZW (F1) males is nonreactive to sex hormones influence. The results obtained corroborate the suggestion of the important role of sex hormones in formation of sex dimorphism and age variability to autoimmunity to type I collagen.

[Genetic aspects of the reaction of humoral immunity to collagen in humans and animals. Report I. Analysis of polymorphism of autoantibody levels to collagen type I in mice NZB X NZW (F1)]. / Geneticheskie aspekty reaktsii gumoral'nogo immuniteta k kollagenu u cheloveka i zhivotnykh. Soobshchenie I. Analiz polimorfizma urovnei autoantitel k kollagenu I tipa u myshei NZB X NZW (F1).

The study of polymorphism of humoral immunoreactions to the type I (AC) collagen in CBA/Lac, C57B1/6-J inbred mice and NZB X NZW (F1) hybrids showed the presence of genetically determined variability of the above mentioned trait. The analysis of intralinear dispersions of AC levels in NZB X NZW (F1) mice revealed sex dimorphism and age variability of the trait. A suggestion was made that sex hormones are important factors in ontogenic formation and modulation of autoimmunity to the type I collagen in NZB X NZW (F1) mice.

[The microsomal oxidative system of NZB and NZW strain mice]. / Mikrosomnaia okislitel'naia sistema myshei linii NZB i NZW.

Content of cytochromes P-450 was lower in liver tissue of NZB mice strain as compared with the NZW strain. Lipid peroxidation in microsomal fraction was higher in females as compared with males (P less than 0.05); it was also higher in females of the NZB mice strain than in the NZW strain (P less than 0.05).

[Chromosome aberrations in F1 (NZBxNZW) mice--an experimental model of systemic lupus erythematosus]. / Khromosomnye aberratsii u myshei F1 (NZBxNZW)--éksperimental'noi modeli sistemnoi krasnoi volchanki.

Spontaneous and mitomycin-C-induced chromosomal aberration level was studied in bone marrow cells of mice F1 (NZBXNZW), an experimental model of systemic lupus erythematosus and in C57BL/6J mice. The chromosome instability level is found to correspond to the degree of the autoimmune process: F1 (NZBXNZW) mice with the highest titer of autoantibodies to DNA has higher chromosomal mutability.