[The mechanism of Na+--K+ pump dysfunction in the erythrocytes of patients with glomerulonephritis and kidney failure]. / Izuchenie mekhanizma disfunktsii Na+--K+-nasosa v éritrotsitakh bol'nykh s glomerulonefritom i pochechnoi nedostatochnost'iu.

The mechanisms of Na, K-ATPase dysfunction in red blood cells of patients suffering from glomerulonephritis and renal failure were studied by means of "functional loads" of an enzyme, enabling not only the activity but also the routine of Na, K-ATPase to be determined. Evidence is provided for the presence of immunotoxic factor on the patients' red blood cells, in the plasma of venous blood and serum of arterial blood. Its excretion from the patients' body and recovery of the routine of Na, K-ATPase have been simulated in vivo and in vitro. The cold immunoglobulin nature of the inhibitor related to the M-chains of IgM was established. It has been shown that the mechanisms of formation of the immunopathogenesis of glomerulonephritis are associated with the appearance of cold autoantibodies and their fragments on blood cell membranes, bringing about a decrease of the activity of Na, K-ATPase and modifying its routine.

[Blocking action of the soluble H-2 antigens and H-2 antibody isolated by various methods and mouse immune lymphocytes]. / Blokiruiushchee deiistvie vydelennykh razlichnymi metodami rastvorimykh antigenov H-2 i H-2-antitela i immunnye limfotsity myshei.

The treatment of tumour and lymphoid cells of mice with 3M KC1 solution having high ionic strength, nonionic detergent and by subsequent freeze-thawing resulted in obtaining serologically active H-2 antigen preparation capable of specifically blocking the cytotoxicity of H-2 antisera. The antigenic activity of the preparations thus obtained depended on the source from which they were isolated (spleen cells and their membrane fragments proved to be the best source), on the degree of maturity of tumor cells and the degree of purification of the preparation, as well as on the methods of solubilization. The blocking action of soluble H-2 antigens on the cytotoxicity of immune lymphocytes depended on the method used for isslating these antigens. The interaction of immune lymphocytes and H-2 antisera with soluble antigens was probably effected by different mechanisms.

[Influence of soluble H-2 antigens on the cytotoxic effect and adsorption of immune lymphocytes]. / Izuchenie vliianiia rastvorimykh antigenov H-2 na tsitotoksicheskii éffekt i adsorbtsiiu immunnykh limfotsitov.

Serologically active preparations of soluble H-2 antigens were obtained by 3 M KCl extraction from the ascitic cells of leukemia L 1210 (H-2d) and sarcoma MX-11 (H-2b). These preparations had no specific influence in vitro on the cytotoxic effect of immune lymphocytes on target cells, and did not inhibit the lymphocyte adsorption on the monolayer of the corresponding target cells.