ABSTRACT
Experiments on 82 rabbits (162 eyes) have shown that RNA hydrolysate phonophoresis possesses a pronounced protective action by stimulating a rapid cupping of metabolic disturbances and a restoration of the chemical state of cellular elements of the retina in a relatively remote terms after the introducing of a monobromoacetate inhibitor (within first 3 days). Experimental therapy beginning 10 days after the introducing of monobromoacetate, i. e. in the period when, besides metabolic disturbances histomorphologic changes were also seen, considerably stimulated activation of compensatory-inflammatory processes. The investigations carried out allow to recommend RNA hydrolysate phonophoresis for clinical examination in dystrophic diseases of the retina.
Subject(s)
Oligoribonucleotides , Phonophoresis , Retinal Degeneration/drug therapy , Ribonucleotides/administration & dosage , Acetates/poisoning , Animals , Drug Evaluation, Preclinical , Female , Histocytochemistry , Male , Rabbits , Retina/drug effects , Retina/metabolism , Retinal Degeneration/chemically induced , Retinal Degeneration/metabolism , Time FactorsSubject(s)
Cornea/drug effects , Oligoribonucleotides , Ribonucleotides/pharmacology , Ultrasonics , Age Factors , Animals , Chinchilla , HistocytochemistryABSTRACT
Reparative and protective properties of the high frequency ultrasound (880 kgHz, 0.4--0.6 W/cm2) have been studied in the experiment performed on rabbits before and after actinomycin D administration (0.2-1.2 mg/kg). RNA and total protein contents have been estimated in cytoplasm of ganglionic cells and in nuclei of photoreceptors. A positive effect of the ultrasound on actinomycin D-induced disorders in metabolic processes of the retina are proved to be weakly manifested. If the antibiotic is used in low concentrations, its inhibiting action can be eliminated or reduced with a subsequent ultrasound treatment of the eye. Simultaneous administration of the ultrasound and increased concentrations of actinomycin D enhances considerably development of certain degenerative changes in the retina. Preliminary ultrasound treatment (especially repeated) of the eye increases metabolic resistivity of retinal cells against the damaging effect of actinomycin D. It is manifested in less amount of cytochemical disorders, and when the inhibitor is applied in small doses-in their absence.
Subject(s)
Adaptation, Physiological , Retina/cytology , Ultrasonics/adverse effects , Adaptation, Physiological/drug effects , Animals , Dactinomycin/pharmacology , Histocytochemistry , Rabbits , Retina/drug effects , Retina/metabolism , Time Factors , Ultrasonic TherapyABSTRACT
By means of quantitative cytochemical methods, changes in contents of protein amino groups on neurons and their surrounding glia have been studied in the rabbit midbrain tectum colliculi and in the cerebral visual cortex after a single and successive stimulation of the animal's eyes with impulsive ultrasound (880 kHz) at various intensity (0.2, 0.4-0.6, 1.0 and 2.0 W/cm2) and exposition for 5 min. The number of mantle gliocytes has been counted. It has been stated that certain metabolic manifestations in the glia overtake considerably (on an average 4-12 h) those in the neurons, and at the ultrasound intensity as great as 0.2 W/cm2 they are registered exclusively in the mantle gliocytes. It is probable that cells of the perineuronal glia, being an intermediate link between the neurons and capillaries, react selectively by changing their protein contents and, thus, perform a leading role in the reaction of the visual analyser to ultrasound stimulations. The ultrasound effect of therapeutic intensity or nearly so, while affecting metabolism of the neurons and neuroglia, does not change their interactions. It is possible that a prolonged active functional state of the neurons is not always ensured by increasing number of their satellite-cells but is realized by some other mechanisms.
