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1.
Equine Vet J ; 49(3): 269-274, 2017 May.
Article in English | MEDLINE | ID: mdl-27783422

ABSTRACT

The aorta can rupture at the aortic root or aortic arch. In most breeds, the aortic root is the likely site and rupture leads to aortocardiac fistula with communication between the aorta and the right atrium, right ventricle and/or the interventricular septum. There is a high prevalence of aortic rupture in young Friesian horses and rupture occurs at the aortic arch with pseudoaneurysm and potentially aortopulmonary fistulation. Echocardiographic and post-mortem techniques must be adapted to identify aortic arch rupture that is not generally identified with standard approaches. Given the narrow genetic base of the Friesian breed and the significant differences found in extracellular matrix composition and metabolism between Friesians and Warmbloods, genetic factors are likely to contribute to the condition in the Friesian breed.


Subject(s)
Aortic Rupture/veterinary , Horse Diseases/pathology , Animals , Aortic Rupture/genetics , Genetic Predisposition to Disease , Horse Diseases/genetics , Horses
2.
Equine Vet J ; 49(5): 609-613, 2017 Sep.
Article in English | MEDLINE | ID: mdl-27859600

ABSTRACT

BACKGROUND: Unlike in Warmblood horses, aortic rupture is quite common in Friesian horses, in which a hereditary trait is suspected. The aortic connective tissue in affected Friesians shows histological changes such as medial necrosis, elastic fibre fragmentation, mucoid material accumulation and fibrosis with aberrant collagen morphology. However, ultrastructural examination of the collagen fibres of the mid-thoracic aorta has been inconclusive in further elucidating the pathogenesis of the disease. OBJECTIVES: To assess several extracellular matrix (ECM) components biochemically in order to explore a possible underlying breed-related systemic ECM defect in Friesians with aortic rupture. STUDY DESIGN: Cadaver study. METHODS: Tissues from affected Friesians (n = 18), unaffected Friesians (n = 10) and Warmblood horses (n = 30) were compared. Samples were taken from the thoracic aorta at the level of the rupture site, from two locations caudal to the rupture and from the deep digital flexor tendon. Total collagen content, post-translational modifications of collagen formation including lysine hydroxylation, and hydroxylysylpyridinoline (HP), lysylpyridinoline (LP) and pyrrole cross-links were analysed. Additionally, elastin cross-links, glycosaminoglycan content and matrix metalloproteinase (MMP) activity were assessed. RESULTS: Significantly increased MMP activity and increased LP and HP cross-linking, lysine hydroxylation and elastin cross-linking were found at the site of rupture in affected Friesians. These changes may reflect processes involved in healing and aneurysm formation. Unaffected Friesians had less lysine hydroxylation and pyrrole cross-linking within the tendons compared with Warmblood horses. No differences in the matrix of the aorta were found between normal Warmbloods and Friesian horses. MAIN LIMITATIONS: Small sample size. CONCLUSIONS: The differences in collagen parameters in tendon tissue may reflect differences in connective tissue metabolism between Friesians and Warmblood horses.


Subject(s)
Aorta, Thoracic/pathology , Aortic Rupture/veterinary , Extracellular Matrix Proteins/metabolism , Horse Diseases/metabolism , Animals , Aortic Rupture/metabolism , Collagen , Glycosaminoglycans , Horses
3.
BMC Res Notes ; 9(1): 405, 2016 Aug 15.
Article in English | MEDLINE | ID: mdl-27527829

ABSTRACT

BACKGROUND: Acquired aortopulmonary fistulation is a rare condition in humans. It usually results as a late complication of a true or pseudoaneurysm of the thoracic aorta. It is most commonly associated with trauma or surgery, less commonly with atherosclerosis, inflammation, hypertension or Marfan's syndrome. Aortopulmonary fistulation is also seen as a rare complication of acute aortic dissection. On rare occasions, acquired aortopulmonary fistulation is reported in aged patients without any of the above mentioned triggering factors. Thus, these cases should be considered as idiopathic aortopulmonary fistulation. Clearly, the pathogenesis of this condition is not yet completely understood. Friesian horses are highly inbred and are affected by several genetic conditions. Rupture of the thoracic aorta has a relatively high prevalence in Friesian horses and is often characterized by the formation of a pseudoaneurysm with subsequent fistulation into the pulmonary artery. Affected animals may survive for several weeks to months. FINDINGS: Here we performed vascular casting in three affected Friesian horses. In all three cases, an aortic rupture at the caudoventral side of the aorta was connected with a rupture of the main pulmonary artery just proximal to its bifurcation. CONCLUSIONS: Affected Friesians show a consistent location and configuration of the aortic rupture site, very similar to the human condition and therefore could act as a spontaneous model to study this disease.


Subject(s)
Arterio-Arterial Fistula/pathology , Pulmonary Artery/abnormalities , Animals , Aortic Rupture/diagnostic imaging , Aortic Rupture/physiopathology , Arterio-Arterial Fistula/diagnostic imaging , Arterio-Arterial Fistula/physiopathology , Coronary Circulation , Disease Models, Animal , Female , Horses , Humans , Male , Pulmonary Artery/diagnostic imaging , Pulmonary Artery/pathology , Pulmonary Artery/physiopathology , Ultrasonics
4.
J Comp Pathol ; 154(2-3): 225-30, 2016.
Article in English | MEDLINE | ID: mdl-26987511

ABSTRACT

Rupture of the aorta is much more common in Friesians compared with other breeds of horse. Rupture always occurs adjacent to the scar of the ligamentum arteriosum. Previous histological examination of ruptured aortic walls suggested the presence of an underlying connective tissue disorder. Therefore, the aim of the present study was to compare the structural characteristics of the tunica media of the mid-thoracic aorta, distant to the lesion, in warmblood and Friesian horses with and without thoracic aortic rupture. In unaffected Friesian horses, the thickness of the tunica media, as well as the percentage area comprised of collagen type I, were significantly higher compared with the warmblood horses, supporting the hypothesis of a primary collagen disorder in the Friesian horse breed. However, in the tunica media of the affected Friesian horses there was no significant wall thickening. Moreover, the percentage area comprised of elastin was significantly lower, while the percentage area comprised of smooth muscle was higher, compared with unaffected Friesian and warmblood horses. These lesions are suggestive of an additional mild elastin deficiency with compensatory smooth muscle cell hypertrophy in affected Friesians.


Subject(s)
Aorta, Thoracic/pathology , Aortic Rupture/veterinary , Horse Diseases/pathology , Animals , Aortic Rupture/pathology , Horses
5.
Vet Pathol ; 52(6): 1142-7, 2015 Nov.
Article in English | MEDLINE | ID: mdl-25367366

ABSTRACT

Megaesophagus appears to be more common in Friesian horses than in other breeds. A prevalence of approximately 2% was observed among Friesian horses presented to the Wolvega Equine Clinic and the Utrecht University Equine Clinic. In this study, morphologic changes in the esophagi of Friesian horses with megaesophagus were compared with those of 6 control horses. Of 18 horses with clinically observed megaesophagus, only 12 animals had esophageal dilation at necropsy, usually involving the thoracic portion. Muscular hypertrophy of the distal esophagus was present in only one-third of the affected horses, indicating that this change is not the most relevant cause of megaesophagus in Friesians. Increased deposition of clumped and disorganized collagen was present in these clinically affected horses mainly in the non-dilated portion of the esophagus. At necropsy, a decrease in neural elements and elastin was present principally in horses with megaesophagus. Mild degeneration and necrosis of the tunica muscularis along the entire length of the esophagus were present in clinically affected horses and encountered only rarely in control animals. There were no significant differences among affected and control horses with respect to inflammation, mineralization, or the number of cells of Cajal. The increased occurrence of megaesophagus in the Friesian breed compared with other horse breeds, together with the presence of abnormal collagen in very young foals, supports the hypothesis that megaesophagus is hereditary in Friesians.


Subject(s)
Esophageal Achalasia/veterinary , Horse Diseases/physiopathology , Animals , Esophageal Achalasia/physiopathology , Esophagus/physiopathology , Female , Horses , Hypertrophy/veterinary , Immunohistochemistry/veterinary , Male , Phenotype
6.
Vet Pathol ; 52(1): 152-9, 2015 Jan.
Article in English | MEDLINE | ID: mdl-24741028

ABSTRACT

Aortic rupture in horses is a rare condition. Although it is relatively common in the Friesian breed, only limited histopathologic information is available. Twenty Friesian horses (1-10 years old) were diagnosed with aortic rupture by postmortem examination. Ruptured aortic walls were analyzed with histology and immunohistochemistry. Based on the histologic and immunohistochemical findings, these cases were divided into 3 groups: acute (n = 4, 20%), subacute (n = 8, 40%), and chronic (n = 8, 40%). Features common to samples from horses in all groups included accumulation of mucoid material; disorganization and fragmentation of the elastic laminae; aortic medial smooth muscle hypertrophy; and medial necrosis of varying degrees, ranging from mild and patchy in the acute cases to severe midzonal necrosis in the chronic cases. Inflammation, most likely secondary to medial necrosis, varied from predominantly neutrophilic infiltrates in the media and periadventitial tissue in the acute group to the presence of mainly hemosiderophages in the periadventitial tissue in the chronic group. Medial fibrosis with aberrant collagen morphology was seen in the subacute group and, more commonly, in the chronic group. Only minimal changes were seen in the aortic vasa vasorum. Smooth muscle hypertrophy and accumulation of mucoid material were not related to the age of the lesions. The findings of this study suggest that a connective tissue disorder affecting elastin or collagen in the aortic media is potentially the underlying cause of aortic rupture in Friesian horses.


Subject(s)
Aneurysm, False/veterinary , Aortic Rupture/veterinary , Arterio-Arterial Fistula/veterinary , Horse Diseases/pathology , Pulmonary Artery/abnormalities , Aneurysm, False/pathology , Animals , Aorta/pathology , Aortic Rupture/pathology , Arterio-Arterial Fistula/pathology , Female , Horses , Immunohistochemistry/veterinary , Male , Pulmonary Artery/pathology , Vasa Vasorum/pathology
7.
Environ Sci Pollut Res Int ; 21(15): 9054-66, 2014.
Article in English | MEDLINE | ID: mdl-24002660

ABSTRACT

Transport and degradation of de-icing chemical (containing propylene glycol, PG) in the vadose zone were studied with a lysimeter experiment and a model, in which transient water flow, kinetic degradation of PG and soil chemistry were combined. The lysimeter experiment indicated that aerobic as well as anaerobic degradation occurs in the vadose zone. Therefore, the model included both types of degradation, which was made possible by assuming advection-controlled (mobile) and diffusion-controlled (immobile) zones. In the mobile zone, oxygen can be transported by diffusion in the gas phase. The immobile zone is always water-saturated, and oxygen only diffuses slowly in the water phase. Therefore, the model is designed in a way that the redox potential can decrease when PG is degraded, and thus, anaerobic degradation can occur. In our model, manganese oxide (MnO2, which is present in the soil) and NO3- (applied to enhance biodegradation) can be used as electron acceptors for anaerobic degradation. The application of NO3- does not result in a lower leaching of PG nor in a slower depletion of MnO2. The thickness of the snowcover influences the leached fraction of PG, as with a high infiltration rate, transport is fast, there is less time for degradation and thus more PG will leach. The model showed that, in this soil, the effect of the water flow dominates over the effect of the degradation parameters on the leaching at a 1-m depth.


Subject(s)
Propylene Glycol/chemistry , Soil Pollutants/chemistry , Biodegradation, Environmental , Diffusion , Kinetics , Models, Theoretical , Water Pollutants, Chemical/chemistry
8.
Equine Vet J ; 45(1): 101-6, 2013 Jan.
Article in English | MEDLINE | ID: mdl-22607232

ABSTRACT

REASONS FOR PERFORMING STUDY: In horses, aortic sinus of Valsalva aneurysms or tears in the aortic root are well-recognised conditions in breeding stallions, often leading to sudden death. A more uncommon form of aortic rupture, located proximal to the ligamentum arteriosum has been reported in 3 Friesian horses. OBJECTIVES: The purpose of this study was to phenotypically characterise aortic rupture and aorto-pulmonary fistulation in Friesian horses in terms of clinical and post mortem data based on 24 cases. METHODS: Friesian horses that were diagnosed with aortic rupture and aorto-pulmonary fistulation over a period of 13 years (1997-2010) at the Department of Equine Sciences of Utrecht University (n = 15) and Wolvega Equine Hospital (n = 9), were included in this study. Case history, results of clinical examination and gross post mortem findings were screened and analysed. RESULTS: Some cases were found dead without prior symptoms, but in several cases signs such as recurrent colic, peripheral oedema and sustained tachycardia were present for several weeks prior to cardiac failure. Clinical examination during hospitalisation revealed increased rectal temperature, peripheral oedema and increased jugular pulse with a bounding arterial pulse. In the majority of horses an aortic rupture of the aortic arch near the ligamentum arteriosum, concurrent with a circumferential cuff of perivascular haemorrhage and aorto-pulmonary fistulation, was found at post mortem examination. CONCLUSIONS: Aorto-pulmonary fistulation in conjunction with aortic rupture is more common in Friesians than previously estimated. In some cases findings demonstrate a progressive pathology rather than acute cardiac failure and sudden death. An appropriate approach is necessary during post mortem examination of the heart in order not to overlook the diagnosis. POTENTIAL RELEVANCE: Equine practitioners should realise that in Friesian horses presented with a history of recurrent false colic, coughing, sustained tachycardia and/or peripheral oedema, aortic rupture and aorto-pulmonary fistulation should be included in the differential diagnosis.


Subject(s)
Aortic Rupture/veterinary , Arterio-Arterial Fistula/veterinary , Horse Diseases/pathology , Animals , Aortic Rupture/pathology , Arterio-Arterial Fistula/pathology , Female , Horses , Male , Pulmonary Artery/abnormalities , Pulmonary Artery/pathology , Retrospective Studies
9.
ISRN Urol ; 2011: 458930, 2011.
Article in English | MEDLINE | ID: mdl-22084800

ABSTRACT

Differences between clinical (cT) and pathological tumor (pT) stage occur often after radical cystectomy (RC) for muscle-invasive bladder cancer. In order to evaluate the impact of downstaging on recurrence and survival, we selected patients from a large, contemporary, population-based series of 1,409 patients with MIBC. We included all patients who underwent RC (N=643) and excluded patients who received (neo)adjuvant therapy, those with known metastasis at time of diagnosis, and those with nonurothelial cell tumors. Disease outcomes were defined as recurrence-free survival (RFS) and relative survival (RS), as a good approximation of bladder cancer-specific survival. After applying the exclusion criteria, 375 patients were eligible for analysis. Tumor downstaging was found to be common after RC; in 99 patients (26.4%), tumor downstaging to non-muscle-invasive stages at RC occurred. Hydronephrosis at baseline and positive lymph nodes at RC occurred significantly less often in these patients. In 62 patients, no tumor was left in the cystectomy specimen. pT stage was pT1 in 20 patients and pTis in 17 patients. Patients with tumor downstaging have about a 30% higher RFS and RS compared to those without. Consequently, tumor downstaging is a favorable marker for prognosis after RC.

10.
Environ Pollut ; 159(1): 198-203, 2011 Jan.
Article in English | MEDLINE | ID: mdl-20932615

ABSTRACT

Effects of C60 nanoparticles (nominal concentrations 0, 15.4 and 154 mg/kg soil) on mortality, growth and reproduction of Lumbricus rubellus earthworms were assessed. C60 exposure had a significant effect on cocoon production, juvenile growth rate and mortality. These endpoints were used to model effects on the population level. This demonstrated reduced population growth rate with increasing C60 concentrations. Furthermore, a shift in stage structure was shown for C60 exposed populations, i.e. a larger proportion of juveniles. This result implies that the lower juvenile growth rate due to exposure to C60 resulted in a larger proportion of juveniles, despite increased mortality among juveniles. Overall, this study indicates that C60 exposure may seriously affect earthworm populations. Furthermore, it was demonstrated that juveniles were more sensitive to C60 exposure than adults.


Subject(s)
Nanoparticles/toxicity , Oligochaeta/drug effects , Animals , Population Dynamics , Soil Pollutants/toxicity
12.
Parasitology ; 123(Pt 3): 309-14, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11578095

ABSTRACT

A simple and robust method for the isolation of gastro-intestinal nematode eggs from faeces is described that uses both salt- and sugar solutions for flotation. Application of this 'salt-sugar' isolation method to large numbers of faecal samples of adult dairy cows indicates a 3- or 4-fold reduction in the proportion of e.p.g.-negative cows relative to studies that used other techniques for egg isolation. The procedure detects more eggs than the Wisconsin flotation method in replicate samples and in spiked egg-free faeces. The number of recovered eggs in spiked faecal samples is linear over a range of egg concentrations, and the transparent faecal preparations that result from the protocol can be stored as digital images which can be used as input for an efficient automated egg-counting procedure. The increased rate of processing of faeces combined with the large reduction of the percentage of e.p.g.-negative cows allows more accurate analysis of large numbers of adult or resistant animals for studies of nematode parasitism.


Subject(s)
Cattle Diseases/parasitology , Parasite Egg Count/veterinary , Trichostrongyloidea/isolation & purification , Trichostrongyloidiasis/veterinary , Animals , Cattle , Cattle Diseases/diagnosis , Feces/parasitology , Female , Image Processing, Computer-Assisted , Parasite Egg Count/methods , Sodium Chloride/chemistry , Sucrose/chemistry , Trichostrongyloidiasis/diagnosis , Trichostrongyloidiasis/parasitology
15.
Pain ; 68(1): 75-78, 1996 Nov.
Article in English | MEDLINE | ID: mdl-9252001

ABSTRACT

Our objective was to study the efficacy of transcutaneous electrical nerve stimulation (TENS) in reducing pain during the first stage of labour. Using a prospective randomized placebo-controlled, double blind clinical trial, a patient-controlled analgesia system was used to measure differences in outcome. Trials took place in a labour unit at the St. Antonius Hospital, Nieuwegein, The Netherlands, during a period of 18 months. Forty-six patients, during the first stage of labour, were treated with TENS, and 48 with a placebo apparatus. Main outcome measures were pain relief, amount of administered analgesics, obstetrical and neonatal outcome, and side effects. No significant differences occurred between groups in the number of requests for pethidine/promethazine. The foetal outcome in both groups was the same. TENS and placebo were considered equally effective by both patients and staff. In conclusion, TENS was not more effective than a placebo apparatus in relieving pain during the first stage of labour. No adverse side-effects occurred.


Subject(s)
Labor Stage, First , Transcutaneous Electric Nerve Stimulation , Adult , Analgesia/methods , Double-Blind Method , Female , Humans , Pain Measurement , Pregnancy , Pregnancy Outcome , Prospective Studies , Self Administration
16.
J Psychosom Res ; 39(2): 183-91, 1995 Feb.
Article in English | MEDLINE | ID: mdl-7595876

ABSTRACT

This article discusses a Dutch questionnaire survey of 60 adult patients and 37 children with Klippel-Trenaunay syndrome (KT), a triad of congenital anomalies characterized by a vascular nevus, varicose veins and bony and soft-tissue hypertrophy. This is the first study known that focuses on the psychological impact of KT. Slow deterioration was found in 40% of adult patients. About 70% report slightly moderate to serious limitations in their daily functioning. Problems in the doctor-patient relationship, as well as psychological problems related to KT, are described. Of the children with KT 75% of the parents report that the condition is stable; 58% of the parents do not report any daily limitations. It is concluded that patients in worse health are suffering from the negative impact and psychological influences of KT. At present an optimal, caring doctor-patient relationship is suggested as the best treatment in some cases for KT.


Subject(s)
Adaptation, Psychological , Klippel-Trenaunay-Weber Syndrome/psychology , Sick Role , Adolescent , Adult , Aged , Child , Child, Preschool , Female , Humans , Infant , Internal-External Control , Male , Middle Aged , Patient Satisfaction , Personality Inventory , Physician-Patient Relations , Quality of Life
17.
Microsc Res Tech ; 25(1): 12-8, 1993 May 01.
Article in English | MEDLINE | ID: mdl-8353303

ABSTRACT

The technique of in situ hybridization has been used to evaluate the expression of an ovulation hormone mRNA (caudodorsal cell hormone; CDCH) in the central nervous system (CNS) of the mollusc Lymnaea stagnalis. Hybridization with radioactive as well as with nonradioactive labeled oligonucleotide and plasmid probes revealed a specific labeling on cell bodies of caudodorsal cells (CDCs), which are known to produce CDCH, on the light microscopical level. In addition, specific labeling was observed outside the cell bodies, as far as the cerebral commissure, where CDCH is released in the haemolymph. To investigate whether these signals represent an axonal localization of the CDCH mRNA, we performed in situ hybridization at the electron microscopical (EM) level. The results showed an intraaxonal localization of CDCH mRNA with digoxigenin labeled oligonucleotide and plasmid probes. Gold labeling was observed in secretion granules, and double labeling experiments showed that these granules also contain CDCH. This specific intragranular localization suggest that CDCH mRNA is transported through the axon and released by exocytosis in the haemolymph.


Subject(s)
Axons/ultrastructure , Invertebrate Hormones/metabolism , RNA, Messenger/ultrastructure , Animals , Axons/metabolism , Central Nervous System/metabolism , Central Nervous System/ultrastructure , Digoxigenin , Female , Immunohistochemistry , In Situ Hybridization , Invertebrate Hormones/genetics , Lymnaea , Microscopy, Immunoelectron , Oligonucleotide Probes , Ovulation , Plasmids , RNA, Messenger/genetics , RNA, Messenger/metabolism
18.
J Cell Sci ; 104 ( Pt 4): 1187-97, 1993 Apr.
Article in English | MEDLINE | ID: mdl-8391016

ABSTRACT

We have determined optimal conditions for the detection of mRNA sequences in cultured cells by nonradioactive in situ hybridization. For this purpose a number of different cell lines have been used: rat 9G cells for the detection of human cytomegalovirus immediate early mRNA, and HeLa as well as 5637 carcinoma cells for the detection of housekeeping gene mRNAs. Extensive optimization of fixation and pretreatment conditions revealed that most intense hybridization signals are obtained when cells are grown on glass microscope slides, fixed with a mixture of formaldehyde and acetic acid, pretreated with pepsin and denatured prior to hybridization. In addition, we also studied the potential of fluorochromized probes for the direct detection of multiple RNA sequences. The optimized in situ hybridization procedure revealed that immediate early mRNA transcripts are, in addition to a cytoplasmic localization, localized within nuclei of rat 9G cells. Double hybridization experiments showed that intron and exon sequences colocalize within the main nuclear signal. In addition, the presence of small, intron-specific, fluorescent spots scattered around the main nuclear signals indicates that intron sequences which are spliced out can be visualized. Additional information about the functioning of cells could be obtained by the detection of mRNA simultaneously with bromodeoxyuridine, incorporated during S-phase, or its cognate protein. The sensitivity of these methods is such that mRNAs of abundantly expressed housekeeping genes can be detected in a variety of cell lines with high signal to noise ratios.


Subject(s)
Cytomegalovirus/genetics , Exons , Introns , RNA, Messenger/analysis , Animals , Bromodeoxyuridine , Cell Line , Fluorescent Dyes , Glyceraldehyde-3-Phosphate Dehydrogenases/genetics , Haptens , Humans , In Situ Hybridization , Molecular Probes , Peptide Elongation Factors/genetics , RNA, Neoplasm/analysis , Tumor Cells, Cultured
19.
J Histochem Cytochem ; 40(11): 1647-57, 1992 Nov.
Article in English | MEDLINE | ID: mdl-1431053

ABSTRACT

The subcellular localization of mRNA sequences encoding neuropeptides in neuropeptidergic cells of the pond snail Lymnaea stagnalis was investigated at the electron microscopic (EM) level by non-radioactive in situ hybridization. Various classes of probes specific for 28S rRNA and for the ovulation hormone (caudodorsal cell hormone; CDCH) mRNA were labeled with biotin or digoxigenin and were detected after hybridization with gold-labeled antibodies. Hybridizations were performed on ultra-thin sections of both Lowicryl-embedded and frozen cerebral ganglia, and a comparison demonstrated that most intense hybridization signals with an acceptable preservation of morphology were obtained with ultra-thin cryosections. Addition of 0.1% glutaraldehyde to the formaldehyde fixative improved the morphology, but on Lowicryl sections this added fixative resulted in a decrease of label intensity. A variety of probes, including plasmids, PCR products, and oligonucleotides, were used and all provided good results, although the use of oligonucleotides on Lowicryl sections resulted in decreased gold labeling. The gold particles were found mainly associated with rough endoplasmic reticulum (RER) but were also observed in lysosomal structures. Finally, the in situ hybridization method presented in this study proved to be compatible with the immunocytochemical detection of the caudodorsal cell hormone, as demonstrated by double labeling experiments.


Subject(s)
In Situ Hybridization/methods , RNA, Messenger/metabolism , RNA, Ribosomal, 28S/metabolism , Animals , Brain/metabolism , Brain/ultrastructure , Endoplasmic Reticulum/metabolism , Endoplasmic Reticulum/ultrastructure , Ganglia/metabolism , Ganglia/ultrastructure , Immunohistochemistry , Lymnaea , Lysosomes/metabolism , Lysosomes/ultrastructure , Microscopy, Electron , Plasmids , Polymerase Chain Reaction , RNA, Messenger/ultrastructure , RNA, Ribosomal, 28S/ultrastructure
20.
Transplantation ; 54(4): 661-4, 1992 Oct.
Article in English | MEDLINE | ID: mdl-1329283

ABSTRACT

Fifty-nine renal transplant recipients were followed during the first 3 months after transplantation. Once weekly, cultures of urine and buffy coat for CMV were taken. Furthermore, peripheral blood leukocytes were examined by an immunocytochemical assay for immediate early antigens of CMV (IEA assay) and by a polymeric chain reaction for CMV DNA. Forty-four patients had a CMV infection; 23 of them were symptomatic. PCR was positive in 22 of the 23 patients with symptomatic CMV disease. For cultures of urine, buffy coat, and the IEA assay these figures were 23, 20, and 21, respectively. The PCR was the first test to become positive in 10 patients. For the cultures of urine and buffy coat and the IEA assay these figures were 0, 2, and 2, respectively. In the other 9 patients, 2 or more tests became positive at the same time. In the patient group with a CMV infection but without symptoms the PCR also had a good correlation with the other diagnostic techniques. These results together with its short processing time of 6 hr make the PCR a sensitive and rapid technique for monitoring CMV infections after renal transplantation.


Subject(s)
Cytomegalovirus Infections/diagnosis , Kidney Transplantation/adverse effects , Polymerase Chain Reaction/methods , Antigens, Viral/blood , Cytomegalovirus/immunology , Cytomegalovirus Infections/epidemiology , Cytomegalovirus Infections/etiology , Humans , Immunohistochemistry , Predictive Value of Tests
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