ABSTRACT
An unusual isozyme of lactate dehydrogenase, originally detected in Kirsten sarcoma virus-infected cells and later shown to be induced in normal mammalian cells by anaerobic shock, has also been reported at elevated levels in several human carcinomas. This enzyme is subject to inhibition by guanosine triphosphate and by the dinucleosides 5',5"'-diadenosine tetraphosphate and 5',5"'-diguanosine tetraphosphate (4). Fluctuation of the activity of this enzyme in soluble extracts of synchronized HeLa cells suggests the enzyme may be linked to DNA synthesis. The lactate dehydrogenase K activity increased in early S phase and then decreased to nearly undetectable levels during the period of most active DNA synthesis. This was observed in cells synchronized by thymidine excess or by aphidicolin, an inhibitor of DNA polymerase alpha.
Subject(s)
Interphase , L-Lactate Dehydrogenase/metabolism , Aphidicolin , DNA Polymerase II/antagonists & inhibitors , DNA Replication , Diterpenes/toxicity , HeLa Cells/drug effects , HeLa Cells/enzymology , Humans , Isoenzymes , Kinetics , Neoplasms/enzymologyABSTRACT
Simian virus 40 chromosomes carry out replicative DNA synthesis in vitro which is sensitive to aphidicolin and to N-ethylmaleimide, resistant to 2',3'-dideoxythymidine-5'-triphosphate, and proportional to the amount of chromosome-associated alpha-like polymerase. Thus, an alpha-like DNA polymerase (alpha polymerase or delta polymerase) is responsible for in vitro DNA synthesis.
Subject(s)
DNA Polymerase II/metabolism , DNA, Viral/biosynthesis , DNA-Directed DNA Polymerase/metabolism , Simian virus 40/enzymology , Aphidicolin , DNA Replication , Dideoxynucleotides , Diterpenes/pharmacology , Ethylmaleimide/pharmacology , Thymine Nucleotides/pharmacologyABSTRACT
We have obtained nearly 1 J of blue-green radiation in 60-nsec pulses by Raman shifting an injection-locked XeCl laser in Pb vapor. The measured 50% Raman energy conversion has been observed in both oscillator and oscillator-amplifier experiments. These results indicate that an XeCl/Pb blue-green laser efficiency of >1.4% should be possible.
ABSTRACT
Medication compliance, knowledge of the disease process, attitudes toward treatment, and lifestyle adjustment were assessed in fifteen maintenance hemodialysis patients. Treatment implications are discussed.
Subject(s)
Patient Compliance , Renal Dialysis/psychology , Adult , Aged , Attitude , Female , Humans , Kidney Failure, Chronic/psychology , Life Style , Male , Middle AgedABSTRACT
The Rotter Inventory was used to assess external vs. internal locus of control in chronic hemodialysis patients. This measure of personality was correlated to psychological and physiologic measures of adaptation to hemodialysis. Patients with high external locus of control had poorer overall compliance with higher interdialytic weight gain, but did not show more psychological distress. Nurses Observation Scale for Inpatient Evaluation correlated negatively with mean K, mean P, Beck Depression rating, and duration of dialysis.
Subject(s)
Kidney Failure, Chronic/psychology , Renal Dialysis/psychology , Adult , Aged , Female , Humans , Male , Middle Aged , Patient Compliance , Phosphorus/blood , Potassium/blood , Stress, PsychologicalABSTRACT
Two DNA polymerases, designated C and N have been characterized in extracts of murine spleen and liver (Evans et al. 1974). The effect of partial hepatectomy on the activity levels of these two enzymes was studied by gel filtration of crude extracts on Sephadex G-200. Both enzymes were observed to respond to the proliferation stimulus. Polymerase N activity reached maximum levels between 30 and 48 hours after hepatectomy. Polymerase C response was delayed until 30 hours and reached maximum values at 48-72 hours.
