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1.
Chemical primer extension in seconds.
Röthlingshöfer, Manuel; Kervio, Eric; Lommel, Tanja; Plutowski, Ulrich; Hochgesand, Annette; Richert, Clemens.
Angew Chem Int Ed Engl ; 47(32): 6065-8, 2008.
Article in English | MEDLINE | ID: mdl-18613189

Subject(s)
Nucleic Acid Amplification Techniques/methods , Pyridines , Catalysis , DNA Primers
2.
Enzyme-free interrogation of RNA sites via primers and oligonucleotides 3'-linked to gold surfaces.
Plutowski, Ulrich; Vogel, Stephanie R; Bauer, Matthias; Deck, Christopher; Pankratz, Michael J; Richert, Clemens.
Org Lett ; 9(11): 2187-90, 2007 May 24.
Article in English | MEDLINE | ID: mdl-17480088

ABSTRACT

The synthesis of a phosphoramidite is described that was used for the preparation of oligonucleotides with a 3'-terminal thiol, linked to the DNA via a SAM-forming undecyl chain and a nonadsorptive tetraethylene glycol unit. A gold surface featuring oligonucleotide probes allowed for label-free in situ mass spectrometric determination of a nucleotide in subpicomole quantities of an RNA transcript.


Subject(s)
Gold/chemistry , Oligonucleotides/chemistry , RNA/chemistry
3.
Chemical primer extension: efficiently determining single nucleotides in DNA.
Hagenbuch, Patrizia; Kervio, Eric; Hochgesand, Annette; Plutowski, Ulrich; Richert, Clemens.
Angew Chem Int Ed Engl ; 44(40): 6588-92, 2005 Oct 14.
Article in English | MEDLINE | ID: mdl-16175646

Subject(s)
DNA Primers/chemistry , DNA/chemistry , DNA/chemical synthesis , Nucleic Acid Amplification Techniques/methods , Nucleotides/chemistry , Base Sequence , Molecular Sequence Data , Nucleotides/analysis , Oligonucleotide Array Sequence Analysis/methods , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Time Factors
4.
A direct glimpse of cross-hybridization: background-passified microarrays that allow mass-spectrometric detection of captured oligonucleotides.
Plutowski, Ulrich; Richert, Clemens.
Angew Chem Int Ed Engl ; 44(4): 621-5, 2005 Jan 14.
Article in English | MEDLINE | ID: mdl-15597393

Subject(s)
Nucleic Acid Hybridization , Oligonucleotide Array Sequence Analysis , Oligonucleotides/analysis , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
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