[Microfluorimetric research on the erythroblastic islands and macrophages of the bone marrow]. / Mikrofliuorimetricheskoe issledovanie éritroblasticheskikh ostrovkov i makrofagov kostnogo mozga.

A microfluorimetric system was used to study rat bone marrow erythroblastic islands (EI) and macrophages. Measurement of fluorescence from acridine-orange-treated cells was performed on the microscope LUMAM-E3 (LOMO, Leningrad) at 530-550 and 630-650 nm. The intensity of fluorescence in 530-550 nm range depended on the intensity of proliferative processes in EI, that at 630-650 nm was associated with activity of lysosomal apparatus of EI macrophages. Erythroblast amplification in EI is parallel both to enhancement of fluorescence intensity at 530-550 nm and 630-650 nm. Intensity of fluorescence of different bone marrow macrophages was evaluated. It is suggested that a part of bone marrow macrophages has high affinity to erythroid tissue.

[The effect of heparin on the process of colony formation from bone marrow mononuclears in a diffusion chamber]. / Vliianie geparina na protsess obrazovaniia kolonii iz kostnomozgovykh mononuklearov v usloviiakh diffuzionnoi kamery.

In the experiments on Wistar rats to whom heparin was injected intravenously in the tail vein in doses of 50, 100 and 250 U, there were studied the colony-forming ability of the hemopoietic cells, the precursors of the bone marrow, and the colony-stimulating activity of the plasma of the heparinized animals 1, 3, 6, 12 and 24 hours after the drug administration. Heparin was found to increase the proliferative activity of the committed myelopoietic cells-precursors on a diffusion microchamber (GM-CFUdc) stimulating the mononuclears and elevating the level of the colony-stimulating activity of the plasma of the experimental animals.

[Bone marrow erythroblast islets in burns]. / Eritroblasticheskie ostrovki kostnogo mozga pri ozhoge.

The character of changes of rat bone marrow (BM) erythroblastic islets (EI) was studied in different periods after III degree burn of 15% body surface. Marked differences in the qualitative EI composition were revealed in the cortical generative zone and the central maturation zone of control animals. Proliferative EI forms predominated in the generative zone and involute EI in the maturation zone. The absolute number of EI in BM reduced in the early period of burn anaemia, probably due to proteolysis mainly through a decrease of involute EI in the maturation zone. The number of EI in BM reached minimal level in the late period of burn anaemia. Inhibition of erythropoiesis in the EI was manifested by a decrease in the content of proliferating forms of EI predominantly in the BM generative zone, probably due to slower involvement of erythroid CFU cells in erythropoiesis and inhibition of specific functions of EI macrophages.

[The quantitative and qualitative characteristics of the reaction of committed bone marrow cell precursors in rats to burn trauma]. / Kolichestvennaia i kachestvennaia kharakteristika reaktsii kommitirovannykh kletok-predshestvennikov kostnogo mozga krys na ozhogovuiu travmu.

The article describes the dynamics of colony-forming units in diffusion chamber of tubular bone marrow compartments 1 and 7 days after a burn. It was found that under normal conditions the activity of the axial part of the bone marrow is twice that of the cortical part. After a burn the inductive capacity of the cortical part becomes equal to that of the axial part. A wider qualitative spectrum of colonies occurs due to the effect of short-distance humoral factors (eosinophils, mast cells).

[Effects of lipoxin B on colony-forming capacity of human peripheral blood mononuclear cells in diffusion chambers]. / Vliianie lipoksina B na kolonieobrazuiushchuiu sposobnost' mononuklearov perifericheskoi krovi cheloveka v usloviiakh diffuzionnoi kamery.

The lipoxin B (5, 14, 15-trihydroxy-6, 8, 10, 12-eicosatetraenoate) obtained by the enzymatic peroxidation of 5, 15-dihydroxy-6, 8, 11, 13-eicosatetraenoate (5, 15-DHETE) with the homogenous rabbit reticulocytes lipoxygenase and purified by HPLC stimulates the proliferation and differentiation of granulocyte-monocyte colony formation units (CFU-GMdc) of human peripheral blood at 3.6 X 10(-9) 3.6 X 10(-8)M in diffusion chamber placed in mouse abdominal cavity. The lipoxin B precursors namely 5,15-DHETE and arachidonic acid had stimulating influence at about 10(-6) and 10(-3)M respectively. The formation of polynuclear cells under the action of the studied lipoxygenase metabolites of the arachidonic acid was shown.