Determination and pharmacokinetic study of ferrocene-A in blood serum of rabbits.

Ferrocene-A (ferrocenyl-1-phenyl-1-dioxy-1,4-butin-2) has significant antitumour and antibacterial properties. This study was designed to estimate main pharmacokinetic parameters of ferrocene-A (FC-A). These parameters are very important from the standpoint to elucidate the mechanism of pharmacological action of ferrocene-A. Examined substance was administrated intramuscularly and orally to rabbits. For determination of small quantities of ferrocene-A in a blood plasma liquid-liquid extraction and reversed-phase high performance liquid chromatography (HPLC) method was applied. Kinetic parameters were estimated using the one-compartment linear pharmacokinetic model.