New disinfectants.

The authors estimated the microbicidal effect of disinfectants produced by the firm Henkel Austria, namely Spitaderm, Incidin M spray, Incidur, Incidur spray, Sekusept pulver, Sekusept forte and Maranon H. The aim of the study was the verification of the effectivity of preparations and the proposal of their application in Czechoslovak practice. The estimation was performed in two stages: in vitro and in practice. The standard suspensive method, the suspensive micromethod and the method of carriers were chosen for the experiments, as well as the strains of grampositive and gramnegative bacteria, the spores of B. subtilis, yeasts and the model virus. In the second part of experiments, the disinfectants were applied according to the producer's instructions in inpatient departments of internal medicine and of surgery at the University Hospital in Olomouc in the course of 4 weeks. During the experiment, the rubdowns were taken twice a week from the working places, floor, instruments and hands and the bacterial contamination of the establishment was estimated. In hemodialysing centre, the apparatuses of the artificial kidney were estimated as well, as the preparation Maranon H is recommended for their disinfection. The results of the experiments show that the recommended concentrations of disinfectants and their effective exposition are chosen with sufficient safety coefficient. In chosen wards, the minimum bacterial contamination was observed. All disinfectants were accepted favourably, their scent estimated as pleasant, the staffs evaluated their high cleaning properties and the dosage equipment, ensuring the precise concentration of preparations. With regard to the above mentioned results, the authors recommend to start the licensed production, as all the tested preparations correspond to the exigent demands of modern and effective disinfection.

[The health status of stomatologists based on the Cornell Medical Index questionnaire]. / Zdravotní stav stomatologu podle dotazníku CMI.

The Cornell Medical Index--CMI was used in a group of 674 stomatologists from the North Moravian region to obtain information on their health status. The control group was formed by 126 health community doctors and factory medical officers from the Olomouc, Sumperk and Prerov districts. The results confirm that stomatologists, a risk group, suffer significantly more from somatic and mental problems, as compared with the control group.

[Microbiological purity of pharmaceutical preparation. Part 1: External remedies; and effects of type of cream, container, storage, temperature and application (author's transl)]. / Mikorbiologische Reinheit der Arzneizubereitungen.

A fractional factorial experiment of the 2n type was carried out, to follow the effects of eight factors affecting simultaneously the microbiological purity of creams (Oleocremor leniens, Hydrocremor stearini) during the manufacturer-consumer chain. The effects of these factors (antibacterial substances, container (collapsible tube, pot), type of cream, storage (open, closed), number of takeouts, manner of taking-out (spatula, finger), temperature of storage, consumer (dermatological clinic, household) and interactive and interdependent. The microbiological purity of the creams is mostly impaired by the number of take-outs and the manner of taking-out. Collapsible tubes and, perhaps even better, one-dose packings are considered a solution of this problem. It is recommended to investigate creams individually with reference to their composition and structure, contents of antibacterial substances, technology and application. The tentative specification for the microbiological purity criteria of ointments and creams, designed for the Czechoslovak Pharmacopoeia, 4th Edition, is acceptable.

[Microbiological purity of pharmaceutical preparation. Part 2: External remedies; the effects of antimicrobial agents in various contaminated creams (author's transl)]. / Mikrobiologische reinheit der arzneizubereitungen. teil 2: externa; wirkung antimikrobieller stoffe in verschiedenen kontaminierten cremetypen.

To study the efficiency of antimicrobial agents in contaminated creams, a complete four-factor trial was performed with Oleocremor leniens, Oleocremor syndermani and Hydrocremor stearini. These creams were prepared with and without the addition of antimicrobial agents (parabene combination, potassium sorbate), contaminated with 5 genera of micro-organisms and bacteriologically examined immediately after preparation and after storage for 14 and 28 d, respectively. It was found that all four factors considered in this trial (type of cream, type of antimicrobial agent, genus of microorganism, time) are interdependent. The efficiency of the antimicrobial agents depends above all upon the type of cream (which acts on the availability of the antimicrobial agents) and upon the micro-organisms. The global recommendation of anti-microbial agents is unsuitable. The test organisms and the test methods as well as the evaluation of their results should be standardized.