ABSTRACT
Experimental data obtained in this study showed the involvement of A. thaliana immunophilin genes At2g16600, At4g33060, and At5g48570 in plant defense responses to the Xanthomonas campestris invasion. We found not only that the expression levels of these genes changed upon bacterial infection, but also that the plant's resistance to the pathogen was increased if the expression levels of the immunophilin genes were elevated in the host cells.
Subject(s)
Arabidopsis/genetics , Peptidylprolyl Isomerase/genetics , Plant Diseases/genetics , Amino Acid Sequence , Arabidopsis/enzymology , Arabidopsis/microbiology , Arabidopsis Proteins/biosynthesis , Arabidopsis Proteins/genetics , Disease Resistance/genetics , Disease Resistance/immunology , Gene Expression Regulation, Plant , Peptidylprolyl Isomerase/metabolism , Plant Diseases/microbiology , Xanthomonas campestris/genetics , Xanthomonas campestris/pathogenicityABSTRACT
A system was created to obtain and select Arabidopsis thaliana genes whose superexpression causes development of a mutant phenotype. Three morphological mutants (two with a markedly retarded growth and one with a fasciated stem) associated with the superexpression of genes At5g10080, At1g33390, and At5g13760 were generated with the use of this system. Localization, structure, and a possible functional organization of these genes were determined.
Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/genetics , Plant Shoots/genetics , Arabidopsis/growth & development , Arabidopsis/metabolism , Arabidopsis Proteins/metabolism , Gene Expression Regulation, Plant , Mutation , Phenotype , Plant Shoots/growth & development , Plant Shoots/metabolismABSTRACT
A system of two vectors, pEnLox and pCre. was developed. The pEnLox vector is used to induce insertional mutations, while pCre is used to obtain transgenic plants expressing the Cre recombinase gene. The T-DNA enhancer is flanked by the loxP sites in the pEnLox vector. As an Arahidopsis thaliana insertional mutant obtained by transformation with pEnLox is crossed with another transgenic line carrying the cre gene. the enhancer sequence is eliminated. The vector system makes it possible to induce insertional mutations and to determine whether the mutant phenotype is caused by the loss-of-function insertional mutation or by overespression of nearby genes in response to the enhancer contained in the insert.
Subject(s)
Genetic Vectors , Plants, Genetically Modified/genetics , Arabidopsis/genetics , Base Sequence , Enhancer Elements, Genetic , Escherichia coli/genetics , Integrases/genetics , Molecular Sequence Data , Mutagenesis, Insertional , Mutation , PlasmidsABSTRACT
The results of genetic and molecular genetic analysis of line 176 of Arabidopsis thaliana with reduced hypocotyls obtained from a previously obtained collection of insertion mutants, are presented. The examined mutation proved to be recessive and based on a single insertion of the T-DNA vector pLD3 into the A. thaliana genome. Computer-aided analysis of the DNA region adjacent to the left border of the insertion revealed a putative site of T-DNA insertion, the 2.5-kb At2g09920 gene located in the long arm of chromosome 2, near the centromere.
