ABSTRACT
The early changes of electrophoretic mobility (EPM) of murine T lymphocytes induced by structural analogues of amixine-dihydrochloryde 4,4'-bis-[2(diethylamino)ethoxy]diphenyl (compound 1) and dihydrochloryde 2-methoxycarbonil-4,4'-bis-[2(diethylamino)ethoxy]diphenyl (compound 2) were studied by electrophoresis technique. During the interval 0-2 hours all compounds increased the absolute values of EPM in comparison with control. These changes were of the same kind--distinctions were quantitative. Amixine and compound 1 during the interval 2-4 hours additionally increased the EPM. The compound 2, on the contrary, decreased the EPM. It was shown that the opposite effects of the aforementioned compounds were caused by the fact that amixine and compound 1 induce, and compound 2 does not induce IFN production in T lymphocytes in vitro. The results of our experiments are important for understanding of the mechanisms of immunomodulating effect of amixine and its structural analogues.
Subject(s)
Biphenyl Compounds/pharmacology , Immunologic Factors/pharmacology , Spleen/drug effects , T-Lymphocytes/drug effects , Tilorone/pharmacology , Animals , Cells, Cultured , Dose-Response Relationship, Drug , Electrophoresis , Interferons/agonists , Interferons/metabolism , Male , Mice , Mice, Inbred CBA , Spleen/cytology , T-Lymphocytes/cytology , Tilorone/analogs & derivativesABSTRACT
Pathological changes during modeling of primary and secondary acute hemorrhagic stroke were studied in rats. We revealed differences in the activity of pharmacological action of medications under condition of acute stroke. The action of medications increased viability of neurons in both hemispheres of rat cerebrum at a right-side primary and secondary hemorrhagic stroke. Following secondary stroke, the amount of degenerative neurons amounted 25.5 +/- 0.8 cells/mm2, following the action ofcerebrolysin this value was 17.6 +/- 1.7 cells/ mm2 and after the action of cortexine and cerebral this value amounted 18.0 +/- 0.9 cells/mm2 and 10.7 +/- 0.4 cells/ mm2, respectively. In control animals the number of degenerative neurons did not exceed 2% and averaged 1.5 +/- 0.1 cells/mm2. Analysis of the morphological and statistical data showed that the most effective remedies under the primary and secondary hemorrhagic insult are cortexine and cerebral. Cerebral was found to be more effective.
Subject(s)
Cerebral Cortex/pathology , Cerebral Hemorrhage/pathology , Cerebrum/pathology , Stroke/pathology , Acute Disease , Amino Acids/pharmacology , Animals , Cerebral Cortex/drug effects , Cerebral Hemorrhage/complications , Cerebral Hemorrhage/drug therapy , Cerebrum/drug effects , Disease Models, Animal , Eosine Yellowish-(YS) , Female , Hematoxylin , Intercellular Signaling Peptides and Proteins , Neurons/drug effects , Neurons/pathology , Neuroprotective Agents/pharmacology , Peptides/pharmacology , Rats , Stroke/complications , Stroke/drug therapyABSTRACT
The influence of extracellular pH on characteristics of volume-activated chloride current, ICl,swell, in the human prostate cancer epithelial cell line, LNCaP, was studied using the patch-clamp technique. Acidification of the extracellular hypotonic solution used to develop the current shortened the temporal parameters of ICl,swell development and reduced its maximal density. Sudden shifts of the pH towards acidification caused fast, transient potentiation of the current followed by its sustained inhibition. Voltage-dependent inactivation of ICl,swell was the most pronounced in the narrow range of pH = 6-7. Based on the analysis of our data we hypothesize that volume-regulated anion channels underlying ICl,swell possess in their structure at least two pH-sensitive molecular groups with similar pK = 6, titration of which modulates the current amplitude and two additional proton-sensitive groups that determine channel's inactivation.
