ABSTRACT
Immunochemical methods have been used to determine the concentration of haptoglobins. The dependence on the phenotype was tested with highly purified Hp 2-1, Hp 2-2 and Hp 1-1, by immunonephelometry and radial immunodiffusion (RID). Measurements with three different instruments: automated immunonephelometer (AIP, Technicon), laser nephelometer (LN, Behring) and immunochemistry system (ICS, Beckman) were performed. For each type of apparatus antisera against a pool of haptoglobins were provided by the respective manufacturers. Some experiments were done with an antiserum to the haptoglobin heavy chain prepared in the laboratory. This study shows that haptoglobin determination depends neither on the physical geometry of the instruments or on the type of antiserum used in this work. In contrast, the data display a dependence on haptoglobin phenotype. When Hp 2-1, the most common phenotype, is taken as a standard, thd values obtained for Hp 2-2 are in good agreement with those obtained for Hp 2-1. However, the values obtained for Hp 1-1 are overestimated unless they are corrected by an experimental factor which has been determined in this study.
Subject(s)
Haptoglobins/analysis , Haptoglobins/immunology , Humans , Immunoassay , Nephelometry and Turbidimetry/methods , Phenotype , Radioimmunoassay/methodsABSTRACT
An assay of human hemoglobin by immunonephelometry-electroimmunodiffusion is proposed. Concentrations in the range from 5 to 180 mg% can be determined to within 2--5% accuracy. The immunonephelometric assay is independent of the presence of haptoglobin whereas the electroimmunodiffusion assay is not. This immunochemical assay, independent of the peroxydase activity of hemoglobin, can be carried out in any biological fluid (plasma, urine, gastric fluid).
Subject(s)
Hemoglobins/analysis , Evaluation Studies as Topic , Humans , Immunoassay , Immunodiffusion/methods , Immunoelectrophoresis/methodsABSTRACT
In 100 patients suffering from intravascular haemolysis, a "protein profile" was made on the basis of measurement of haptoglobin, haemopexin and orosomucoid. The estimations were carried out using a rapid precise automatic technique using monospecific immune serum. The results obtained indicate that these tests are sensitive and reliable.
