Protective Effect of Turmeric against Bisphenol-A Induced Genotoxicity in Rats.

In this study, the protective role of turmeric on genotoxic effects of Bisphenol-A exposure in Wistar rats by in vivo experiment were investigated. Bisphenol-A is a known endocrine disruptor and suspected carcinogen, that comes diet through plastics for food packaging and food processing. In this study, rats were divided into three groups of twelve animals each and were administered with Bisphenol-A by oral gavage with levels of 0, 50 and, 100 µg. Half of the animals in each group were fed with feed which contained 3% turmeric (wt/wt), for a period of 4 wk, while the rest of the rats received the same diet treatment without the addition of turmeric. At the end of the experiment, all rats were terminated and the internal organs such as liver, kidney, femurs were collected and analyzed. Mean and SD values were compared by one-way ANOVA and Kruskal-Wallis-Wilcoxon test, the formation of micronuclei was compared using Mann-Whitney U-test. Significant decrease in serum malondialdehyde and urinary 8-hydroxy-2'-deoxyguanosine levels were observed in Bisphenol-A+turmeric groups as compared to Bisphenol-A groups. Bisphenol-A groups exhibited significantly higher mean levels of DNA damage in liver and kidney as compared to the untreated control group. Bisphenol-A group showed significant increase in the formation of micronuclei which was approximately threefold higher as compared to the control group. A significant decrease in DNA migration was observed in Bisphenol-A+turmeric fed groups in liver and kidney. Turmeric feeding significantly inhibited the micronuclei formation induced by Bisphenol-A. The study results indicate that turmeric can protect against Bisphenol-A induced genotoxicity in rats.

Effect of oxytocin injection to milching buffaloes on its content & stability in milk.

BACKGROUND & OBJECTIVES: Oxytocin (OT) injections to milch cattle for milk letdown have become a common practice amongst dairy farmers in India. Although there is no reported evidence, it is widely presumed that long term consumption of such milk leads to adverse health consequences. However, there is no information on the effect of exogenous OT injections on milk OT content and its stability during heating and gastrointestinal digestion. This study was carried out to determine the OT content in milk samples given by buffaloes with and without OT injections and to assess the stability of OT in the milk. METHODS: Milk samples from milch buffaloes (Murrah buffalo) were collected from local farmers with (n=121) or without (n=120) exogenous OT injections during 3 to 5 months of lactation period. The OT content of milk samples was estimated by competitive enzyme immunoassay (EIA). The thermal and digestive stability of OT was assessed by in silico and in vitro digestion methods. RESULTS: The OT content of the milk samples was similar regardless of OT injections used. Further, OT was found to be stable to heat treatment and gastric pepsin digestion while it was rapidly digested during the simulated intestinal digestion. Reduced OT was digested by pepsin, implying that internal disulphide bridge of OT rendered the peptide resistant to peptic digestion. On the other hand, phenylmethylsulphonyl fluoride (PMSF), a serine protease inhibitor, abrogated the pancreatin induced digestion of OT. INTERPRETATION & CONCLUSIONS: These findings suggest that exogenous OT injections do not influence its content in milk. Further, OT present in milk is rapidly degraded during intestinal digestion, ruling out its intestinal absorption and associated adverse health consequences, if any.

Assessment of dietary intakes of nineteen pesticide residues among five socioeconomic sections of Hyderabad--a total diet study approach.

Total diet study approach was used to assess the dietary intakes of pesticide residues among the select population in Hyderabad. When assessed by a food frequency questionnaire, it was found that the food intakes varied among five socioeconomic sections (SES). Therefore, we intended to compare the intakes of pesticide residues through these foods among the five SES. A total of 195 foods from different markets were collected and analyzed for 19 pesticides. The residues were analyzed with a gas chromatograph and were confirmed with mass spectrometry. About 51 % of the samples were detected with one or more residues. Thirteen out of the 19 residues were present in levels above detection limits in various concentrations. The median concentrations of the residues in all the samples tested, ranged from 0.00010 to 0.33 mg/kg. Highest median concentration was for ß-HCH in water samples. Exposures to all the residues were below the respective ADIs at both mean and 95th percentile levels of food intakes with highest estimated dietary intakes (EDIs) of ß-HCH in both the cases. The EDIs of ß-HCH were the highest among all the residues at both the intake levels among all the SES. The EDIs of ß-HCH were significantly higher in lower SES than higher SES possibly due to the consumption of rice cooked in water contaminated with ß-HCH. EDIs for other residues did not differ significantly among the five SES.

Dose-dependent effect in the inhibition of oxidative stress and anticlastogenic potential of ginger in STZ induced diabetic rats.

Ginger is an important medicinal herb has numerous bioactive components and is used in the management, control and/or treatment of diseases including diabetes mellitus. The present study was undertaken to see the dose-response effect of ginger and evaluate the possible protective effects of dietary ginger on oxidative stress and genotoxicity induced by streptozotocin (STZ) diabetic rats. Inbred male Wistar/NIN rats of 8-9 weeks old were treated with 30 mg/kg of STZ. Rats were divided into different groups of control, diabetic non-treated, and diabetic treated with ginger powder at 0.5%, 1% and 5% respectively. After feeding for a month, blood and tissues were collected to see the effect of ginger on antioxidant status, DNA damage and bone marrow genotoxicity. In this study ginger exerted a protective effect against STZ-induced diabetes by modulating antioxidant enzymes and glutathione and down regulating lipid and protein oxidation and inhibition in genotoxicity in a dose-response manner.

Inhibition of lung tumor development by berry extracts in mice exposed to cigarette smoke.

Cigarette smoke (CS) and dietary factors play a major role in cancer epidemiology. At the same time, however, the diet is the richest source of anticancer agents. Berries possess a broad array of health protective properties and were found to attenuate the yield of tumors induced by individual carcinogens in the rodent digestive tract and mammary gland but failed to prevent lung tumors induced by typical CS components in mice. We exposed whole-body Swiss ICR mice to mainstream CS, starting at birth and continuing daily for 4 months. Aqueous extracts of black chokeberry and strawberry were given as the only source of drinking water, starting after weaning and continuing for 7 months, thus mimicking an intervention in current smokers. In the absence of berries, CS caused a loss of body weight, induced early cytogenetical damage in circulating erythrocytes and histopathological alterations in lung (emphysema, blood vessel proliferation, alveolar epithelial hyperplasia and adenomas), liver (parenchymal degeneration) and urinary bladder (epithelial hyperplasia). Both berry extracts inhibited the CS-related body weight loss, cytogenetical damage, liver degeneration, pulmonary emphysema and lung adenomas. Protective effects were more pronounced in female mice, which may be ascribed to modulation by berry components of the metabolism of estrogens implicated in lung carcinogenesis. Interestingly, both the carcinogen and the chemopreventive agents tested are complex mixtures that contain a multitude of components working through composite mechanisms.

Focus group studies on food safety knowledge, perceptions, and practices of school-going adolescent girls in South India.

OBJECTIVE: To understand food safety knowledge, perceptions, and practices of adolescent girls. DESIGN: Focus group discussions (FGDs) with 32 groups selected using stratified random sampling. SETTING: Four South Indian states. PARTICIPANTS: Adolescent girls (10-19 years). PHENOMENA OF INTEREST: Food safety knowledge, perceptions, and practices. ANALYSES: FGDs were audio recorded, transcribed, and manually coded by topic. At each stage of coding and analysis, reports were read independently by 3 researchers. Results were presented according to 4 food safety topics: concept of safe food; home-cooked food or outside food; packaged food products and food labels; and previous exposure to food safety education. RESULTS: Subjects confused the concept of nutrition with food safety. They were checking food labels, but they were not aware of quality symbols like ISI (Bureau of Indian Standards), FPO (Fruit Products Order), and AGMARK (Agriculture Marking and Grading Act); trusted more in brand names/expensive packaged food; were less careful about snack food safety. Receiving food safety information through school health education was preferred. CONCLUSIONS AND IMPLICATIONS: Adolescent girls are not aware of food quality standards. Awareness needs to be raised on permitted food additives concerning which foods can use them. Children should be familiarized with quality symbols on food labels.

Food safety knowledge, attitudes and practices of mothers: findings from focus group studies in South India.

In India, most of the diarrhoeal deaths among children (<5 years) are attributed to food and water contamination. Mothers are usually the final line of defence against food borne illnesses. Thus, the role of mothers in ensuring food safety at homes is well accepted. There are hardly any studies in India to understand their knowledge, attitudes and practices on food safety. The present study was an attempt in this direction. A total of 32 Focus Group Discussions were carried out with mothers of children <5 years in 16 districts from all the four South Indian states. The findings reveal that food safety awareness and practices are good among mothers perhaps due to the Indian food ethos passed on to them through generations. Home cooked foods are considered to be safer than prepared foods bought from outside. Many mothers were aware of the common food adulterants but do not bother to complain or take action. There is a need to create enabling environment with improved access to potable water, sanitation and cooking fuel. Spreading awareness about checking food labels and reporting to the health authorities in case of food poisoning or adulteration is also the need of the hour. The Anganwadi Centres can be the focal points for imparting food safety education to the mothers.