ABSTRACT
A study to evaluate the natural rate of infection of Ixodes ricinus with Borrelia burgdorferi sensu lato was carried out in an endemic focus of Lyme disease in the Trieste area in northern Italy. Two-hundred and twenty-seven ticks collected in ten different stations were tested individually for the presence of the spirochetes using polymerase chain reaction techniques able to identify both Borrelia burgdorferi sensu lato and the four genospecies (Borrelia burgdorferi sensu stricto, Borrelia garinii. Borrelia afzelii and group VS116). Multiple infection of individual ticks was found. The infection rate ranged from 0-70%. Infection of Ixodes ricinus with Borrelia burgdorferi group VS116 was found for the first time in Italy in both a high and a low endemic focus of Lyme disease.
Subject(s)
Borrelia burgdorferi Group/isolation & purification , Borrelia burgdorferi , Borrelia/isolation & purification , Endemic Diseases , Ixodes/microbiology , Lyme Disease/epidemiology , Animals , Borrelia/classification , Borrelia/genetics , Borrelia burgdorferi Group/classification , Borrelia burgdorferi Group/genetics , DNA, Bacterial/analysis , Humans , Italy/epidemiology , Lyme Disease/microbiology , Polymerase Chain Reaction , Prevalence , Species SpecificityABSTRACT
This study, carried out in the Friuli-Venezia Giulia Region (N.E. Italy), concerns the traditional uses of wild plants against human diseases. One hundred eighty-one local plant species are used for treating various illnesses. Parts of plants, their traditional uses, methods of preparing the drug, chemical constituents and therapeutical properties are described. Often the therapeutical properties are correctly known. This study may be useful to pharmacologists and chemists interested in plants with medicinal properties, as well as to botanists with ethnobotanical interests.
