ABSTRACT
Breast tumor diseases include a wide range of pathologies that require different approaches to their treatment. MicroRNA (miR) levels, reflecting regulation of the gene expression involved in tumorigenesis, can be diagnostic and prognostic markers of breast diseases. The levels of circulating miR-181a and miR-25 were measured in patients with benign breast diseases (BBD), patients with invasive carcinoma of a nonspecific type (ICNT) and also in conditionally healthy women. Expression of both miRs was higher in patients of both groups as compared to controls; at the same time, the content of serum miR-181a and miR-25 was higher in BBD patients than in ICNT patients. The detected changes may be of interest in the context of precancerous changes in BBD. It seems possible to use them in the future as markers of the pathological process as a part of a large diagnostic panel.
Subject(s)
Breast Diseases , MicroRNAs , Humans , Female , MicroRNAs/genetics , MicroRNAs/metabolism , Breast Diseases/diagnosis , Breast Diseases/geneticsABSTRACT
The study revealed high levels of antibodies class A and G to benzopyrene and antibodies class G to estradiol and progesterone in women with pathology of fetus development. The most informative indicators of high risk of development of reproductive pathology are established The recommendations concerning the application of antibodies to benzopyrene, estradiol and progesterone in diagnostics of inherent malformations of fetus are provided.
Subject(s)
Antibodies , Benzo(a)pyrene , Estradiol/immunology , Fetal Diseases , Immunoglobulin A , Immunoglobulin G , Progesterone/immunology , Adult , Antibodies/blood , Antibodies/immunology , Female , Fetal Diseases/blood , Fetal Diseases/diagnosis , Fetal Diseases/immunology , Fetus/abnormalities , Humans , Immunoglobulin A/blood , Immunoglobulin A/immunology , Immunoglobulin G/blood , Immunoglobulin G/immunology , PregnancyABSTRACT
AIM: To develop experimental model for definition of immunological images of chemical carcinogens. MATERIALS AND METHODS: The conjugates of benzo[a]pyrene, benz[a]anthracene, anthracene, chrysene and pyrene with bovine serum albumin and yeast hexokinase were synthesized. Rabbits were immunized by bovine serum albumin-hapten conjugates. Antibodies to each hapten were isolated from the serum by affinity chromatography with the hapten-yeast hexokinase-Sepharose sorbents. The binding of each hapten with each antibody was determined by competitive immunoassay. RESULTS: The immunological images of all the investigated chemical compounds were described. CONCLUSION: The model is proposed to determine the internal immunological images of anti-idiotypic monoclonal antibodies to chemical carcinogens.
