[The efficiency of the enzyme immunoassay test system opisthorchiasis-CIC-EIA-best to detect circulating immune complexes containing opisthorchis antigens in the serum of patients with opisthorchiasis].

The efficacy of a kit of Opisthorchiasis-CIC-EIA-Best reagents was evaluated using 270 sera from patients in the study and control groups. The kit showed a sufficient sensitivity (not less than 87.2%) and a high specificity (not less than 97.9%). The use of the above kit of the reagents for enzyme immunoassay in practical healthcare enables one to increase detection rates among the infested subjects on comprehensive examination of those with suspected opisthorchiasis.

[Use of an enzyme immunoassay test system with cystic Echinococcus antigen to diagnose echinococcosis alveolaris (multilocularis) (alveococcosis)].

Twenty-three serum samples from 12 patients with Echinococcus alveolaris collected 1 to 12 years after surgery and 1--13 courses of specific therapy were used to evaluate the diagnostic effectiveness of an enzyme immunoassay (EIA) test system with cystic Echinococcus antigen. The findings enable the EIA test system with cystic Echinococcus antigen to be recommended for the diagnosis of Echinococcosis alveolaris and for the monitoring of its treatment results.

[Optimization of serological diagnosis of cystic echinococcosus (granulosus) infection].

The diagnostic effectiveness of instrumental analysis of the concentration of specific antibodies, which was expressed in antibody units and titers, was compared in the test system of enzyme immunoassay using Echinococcus granulosus on 76 serum samples from patients with echinococcosis. The factors of possible influence on the instrumental readings of enzyme immunoassay are presented. A correlation was found in the specific antibody concentrations expressed in antibody units and titers.

[Consideration of the results of enzyme immunoassay in tissue helminthisms in antibody units].

Two hundred and twenty-two serum samples from patients infected with Toxocara, 374 from those infected with Echinococcus alveolaris, and 200 from healthy blood donors, which were screened in the enzyme immunoassay system, were used to assess an instrumental method for considering the assay results expressed in antibody units (AU), A direct correlation was found between the values of the intensity of an immune response in the infected, which were expressed in AU and titers routinely used for this purpose. Introducing the optic density index, standardized by the manufacturer of a reference test-positive sample kit, into the formula to calculate AU reduces possible variations in the assay results both between different batches and within one batch of kits.

[The epidemiological-epizootiological subdivision of the nosogeographic range of trichinosis]. / Epidemiologo-épizooticheskoe raionirovanie nozoareala trikhinelleza.

The prevalence of trichinosis was analyzed among human beings and animals in Moscow Province over 50 years (1946-1996). A total of 7022 domestic, synanthropic, and wild carnivorous animals were incompletely helminthologically dissected. Enzyme immunoassay revealed diagnostic Trichinella antigen antibody titers in 45.4% of 544 examinees during outbreaks and at their intervals, as well as in 0.58% of domestic pigs of different property forms. Trichinella larvae were found in domestic pigs (0.13%), dogs (12.3%), cats (12.9%), hunting and fur-bearing (wild boars (2.0%), wolves (38.8%), red foxes (32.0%), raccoon-like dogs (20.0%)) animals and rats (2.4%). Criteria were developed and epidemiological and epizootic division of the districts near Moscow was made. Three unfavourable (hyperendemic, mesoendemic, hypoendemic) and one favourable areas were identified. A trichinosis control programme was worked out.

[The efficiency of the serological reactions with Trichinella spiralis somatic antigen in the diagnosis of human trichinelliasis caused by infection with natural and synanthropic Trichinella isolates]. / Effektivnost' serologicheskikh reaktsii s somaticheskim antigenom Trichinella spiralis v diagnostike trikhinelleza cheloveka, vyzyvaemom pri zarazhenii prirodnymi i sinantropnymi izoliatami trikhinell.

The efficiency of serological tests (enzyme immuno-assay (EIA) and indirect hemagglutination test (IHAT)) using the antigen T.spiralis was examined on 318 sera from trichinosis patients infected via pork meat and on 112 sera from those infected via wild animal meat. This was estimated at 85.6 and 84.8% with EIA and 93.0 and 85.7% with IHAT in the two groups, respectively. To enhance the efficiency of serological diagnosis of trichinosis, it is recommended that EIA and IHAT should be used concurrently.

[The effectiveness of 2 immunoenzyme analysis test systems in the diagnosis of human trichinelliasis]. / Effektivnost' dvukh test-sistem immunofermentnogo analiza v diagnostike trikhinelleza cheloveka.

The efficacy of 2 test systems for enzyme immunoassay using various ingredients (produced in the E. I. Martsinovskii Institute of Medical Parasitology and Tropical Medicine, USSR Ministry of Public Health and Institute of Advanced Medical and Pharmaceutical Training, Prague) has been assessed on blood sera of 122 patients with trichinellosis, 200 patients with other parasitic diseases and 300 healthy donors. No considerable differences in the efficacy of 2 test systems have been revealed. The test sensitivity is higher with the use of somatic than secretory-excretory antigens of identical specificity.