ABSTRACT
Um indivíduo adulto normal apresenta em seus eritrócitos, 96 a 98 por cento de hemoglobina tipo A(HbA - a2b2), 2,0 a 3,7 por cento tipo A2 (HbA2 - a2d2). Os níveis de HbF podem estar anormalmente elevados em condições hereditárias ou adquiridas. Este trabalho teve como objetivo, estabelecer o perfil hemoglobínico do paciente portador do HIV, e observar as possíveis alterações fenotípicas causadas pelo referido vírus, por medicamentos usados no tratamento de tal infecção, em função dos estágios da doença ou de algumas destas interações. Foram estudados
Subject(s)
HIV , Hemoglobins , Pharmaceutical PreparationsABSTRACT
Este artigo retrata as principais classificações das anemias: do ponto de vista laboratorial (classificação morfológica) e etiológico (classificação fisiopatológica). Além disso, tenta agrupá-las com base em ambos os critérios (classficação morfo-etiológica) e avaliar de que modo uma visão global das anemias em seus vários subgrupos pode ajudar no diagnóstico de tais entidades clínicas
Subject(s)
Anemia , Hematologic DiseasesABSTRACT
A coloração de Perls é de grande utilidade para identificar ferro em forma de grânulos no interior dos EritrobLastos (denominados sideroblastos), nos histiócitos e macrófagos, além do ferro intersticial. É muito útil no diagnóstico diferencial de várias patologias hematológicas. Assim, propusemos-nos a modificar algumas fases desta técnica a fim de otimizar a coloração do ferro nas células e interstício. Na primeira fase, o esfregaço sanguíneo que era fixado em vapor de formol por 30 minutos e lavado várias vezes em água, passou a ser fixado por 40 minutos sem lavagem subsequente em água, diminuindo o risco de danos (lise) ao esfregaço. A fase de coloração com ferrocianeto ácido contracoloração com Fast Red Nuclear, aumentamos a concentração...
Subject(s)
Staining and Labeling , Iron , MethodsABSTRACT
Fetal hemoglobin was measured in HIV 1/2 patients under treatment with combined therapy (zidovudine and a protease inhbitor). A total of 143 patientes and 103 normal individual were investigated by the quantitative method of Betke and the semi-quantitative acid elution method of Keihauer. In the normal person, hemoglobin F makes up less than.
Subject(s)
HIV-1 , HIV , HIV-2 , Fetal Hemoglobin , Hemoglobins , ZidovudineABSTRACT
Fetal hemoglobin was measured in HIV1/2 patients under treatment with combined therapy (zidovudine and a protease inhibitor). A total of 143 patients and 103 normal individuals were investigated by the quantitative method of Betke and the semi-quantitative acid elution method of Kleihauer. In the normal person, hemoglobin F makes up less than 1 percent and an increase higher than 1.5 percent was observed in 21.4 percent of HIV patients by the method of Betke and in 24.8 percent of HIV-infected patients by the method of Kleihauer. The quantitative biochemical method of Betke showed that the populations were significantly different (two-tailed Mann-Whitney test). The reason for this hemoglobin F increase might be ascribed to the effect of zidovudine or to direct viral action on gamma chain expression. The finding of a higher F cell frequency indicated by the method of Kleihauer rather suggests that there is an increased F cell clone proliferation rather than an increase in hemoglobin F level in every cell
Subject(s)
Humans , Fetal Hemoglobin/analysis , HIV Infections/blood , Anti-HIV Agents/therapeutic use , Case-Control Studies , Chi-Square Distribution , Erythroid Precursor Cells/drug effects , HIV Infections/drug therapy , HIV Protease Inhibitors/therapeutic use , Statistics, Nonparametric , Zidovudine/therapeutic useABSTRACT
Fetal hemoglobin was measured in HIV1/2 patients under treatment with combined therapy (zidovudine and a protease inhibitor). A total of 143 patients and 103 normal individuals were investigated by the quantitative method of Betke and the semi-quantitative acid elution method of Kleihauer. In the normal person, hemoglobin F makes up less than 1% and an increase higher than 1.5% was observed in 21.4% of HIV patients by the method of Betke and in 24.8% of HIV-infected patients by the method of Kleihauer. The quantitative biochemical method of Betke showed that the populations were significantly different (two-tailed Mann-Whitney test). The reason for this hemoglobin F increase might be ascribed to the effect of zidovudine or to direct viral action on gamma chain expression. The finding of a higher F cell frequency indicated by the method of Kleihauer rather suggests that there is an increased F cell clone proliferation rather than an increase in hemoglobin F level in every cell.
