[A new cell line of rat gasserian ganglion neurinoma NGUK-1]. / Novaia kletochnaia liniia nevrinomy gasserova uzla krysy NGUK-1.

A new cell line has been derived from the rat gasserian ganglion neurinoma NGUK-1 induced by transplacental administration of ethylnitrosourea. It is characterized by an astrocyte-like growth pattern at the low cell density, and by an epitheliocyte-like pattern in the confluent monolayer. The cell line displays a high proliferative activity, its maximum mitotic index and proliferative pool being--2.5 and 96%, respectively. The chromosome number ranges between 20 to 100. The chromosome modal number is 39--44. The new cell line has been used for the express diagnostic of rabies, for determination of serum glial toxicity in neurologic patients, and interferon titration.

[Formation of microfilament bundles in the tumor cell interphase nuclei of a rat neurinoma exposed to dimethyl sulfoxide]. / Formirovanie tiazhei mikrofilamentov v interfaznykh iadrakh opukholevykh kletok nevrinomy krysy pri deistvii dimetilsul'foksida.

The action of 15% dimethyl sulfoxide (DMSO) on the ultrastructure of the rat neurinoma cells (line NGUK-I) has been studied. The agent induced the formation of microfilament bundles in interphase nuclei after 30-60 min of treatment. The microfilament bundles revealed are suggested to be actin.

[Effect of dimethylsulfoxide on the morphology and proliferation of the tumor NGUK-1 cell line]. / Deistvie dimetilsul'foksida na morfologiiu i razmnozhenie opukholevykh kletok linii NGUK-1.

The effects of low doses of dimethyl sulfoxide (DMSO) on the growth and morphology of tumour NGUK-1 strain cells from neurinoma were studied. DMSO produced a dose-dependent reduction in the proliferative capacity. 3% and 5% DMSO concentration inhibited the mitotic activity in the culture and the entry of cells into S-phase of the cell cycle, which was demonstrated by decreased mitotic and thymidine-labelling index. Electron microscopic studies at these DMSO concentrations have revealed large cisterns of rough endoplasmic reticulum with electron dense fine granules. Nucleoli had a spongy structure. DMSO induced stimulation of protein synthesis in cells. At greater DMSO concentrations almost all the cells died. At a 1% concentration DMSO had no effect on cellular morphology and proliferation of in vitro propagated tumour cells.

[Ultrastructural expression of the process of cell fusion in a rat neurinoma culture exposed to polyethylene glycol]. / Ul'trastrukturnoe vyrazhenie protsessa sliianiia kletok kul'tury nevrinomy krysy pri deistvii poliétilenglikolia.

The time course of cell fusion was examined in experiments made with a new cell line derived from rat neurinoma. Exposure to polyethylene glycol (PEG) combined with dimethylsulfoxide led to absorption of cells on plasmalemmas, modification of glycocalyx, disorganization of plasmalemmas in local zones of adjacent membranes, and formation of the common zones of the cytoplasm between neighboring cells. The method used promoted the obtaining of polykaryons, which was accompanied by a decrease in PEG cytotoxicity.

[The course of pregnancy and diabetes mellitus in pregnant women with a long-standing disease]. / Osobennosti techeniia beremennosti i sakharnogo diabeta u beremennykh s bol'shoi davnost'iu zabolevaniia.

The course of pregnancy and treatment of diabetes mellitus during pregnancy were studied in 111 patients (119 pregnancies), who had fallen ill with diabetes mellitus before 5 (11 cases), at the age of 6-10 (39 cases), and at the age of 11-15 (61 cases). There were no patients with mild forms of the disease; 41.6% patients had diabetes mellitus of medium severity, and 58.4% patients grave forms of the disease. Diabetic retinopathy was observed in 47% patients, diabetic nephropathy in 13% patients, signs of acute and chronic pyelonephritis in 8.8% patients. Perinatal mortality in the group under observation was 13.4%. In the patients who had fallen ill with diabetes mellitus later it was 5.7%, and it was observed only in unstable and unsatisfactory compensation of diabetes mellitus. In women who had suffered diabetes since childhood and adolescence, hydramnion and toxemia of pregnancy were recorded in 40% cases.

[NAG infection in Rana temporaria with suppressed normal intestinal microflora]. / NAG-infektsiia u travianykh liagushek na fone podavleniia normal'noi kishechnoi mikroflory.

Frogs of the Rana temporaria species with disturbed biocenosis induced by combined effects of hypothermia (+4 degrees C) and tetracycline were used as an experimental model. The animals were inoculated orally with cultured NAD-vibrios and subjected to clinical, bacteriological, immunomorphological and electron microscopical examinations. The lymph-blood system was shown to be a possible pathway for generalization of the infection. Several causes of long-term persistence of the vibrios in Rana temporaria are discussed.

[NAG-infection in grass frogs (Rana temporaria) subjected to hypothermia]. / NAG-infektsiia u travianykh liagushek (Rana temporaria), podvergshikhsia gipotermii

Rana temporaria kept under hypothermic conditions approaching anabiosis were inoculated with NAG-vibrios and examined clinically, bacteriologically, histologically, and electron microscopically. Oral inoculation of hypothermic frogs with NAG-vibrios resulted in 18 to 24 hours in the development of acute NAG-infection resembling the cholera-like syndrome, and characterized by general intoxication and local enteropathogenic effects. NAG-vibrios persisted in the frog gastrointestinal tract for a long time after the cessation of the acute period of the disease.