ABSTRACT
Time-course of induced accumulation of callose in tomato cells has been studied. Localization of callose in L. esculenthum cells was investigated by fluorescent microscopy technique, and the optimal time for its determination was found. Callose accumulation in tomato cells treated with different biotic elicitors was determined. Nonlinear dependence between callose accumulation and concentration of chitin oligomers (with 3-5 N-acetylglucosamine fragments) was established. Increasing of callose accumulation in tomato cells was proportional to the increase of concentration ofchitin dimer and chitosan in the culture medium.
Subject(s)
Acetylglucosamine/pharmacology , Cell Membrane/metabolism , Chitosan/pharmacology , Glucans/metabolism , Solanum lycopersicum/drug effects , Solanum lycopersicum/cytology , Solanum lycopersicum/genetics , Solanum lycopersicum/metabolism , Microscopy, Fluorescence , Time FactorsABSTRACT
The effects of beta-aminobutiric acid as an inductor of plant resistance was studied. The influence of BABA pretreatment on A. cepa plants of three different pathogen-resistant varieties was investigated by fluorescence microscopic methods. It was determined that BABA is able to prime the plant callose accumulation.
