Verification of High-Rate Vertical Loading Laboratory Skeletal Fractures by Comparison with Theater Injury Patterns.

For the current study, an existing theater injury data set was compared to component and whole body experiments meant to replicate the theater high rate vertical loading environment. The theater injury data set was derived from real world events that were within the design range of the Warrior Injury Assessment Manikin. A qualitative and quantitative assessment of the whole body fracture patterns was developed to determine whether the laboratory loading was correctly representing the resulting injuries seen in theater Underbody Blast (UBB) events. Results indicated that most of the experimental test fracture patterns were similar to the theater injuries for Abbreviated Injury Scale body regions of interest (lower extremities, pelvis, and spine); however, some of the body regions had higher similarity scores compared to others. Whole body fracture distribution was less similar than the component tests because of differences in injury distributions. The lower extremity whole body similarity was lower than spine and pelvis similarity. This analysis was able to identify some experimental tests that might not represent theater loading. In conclusion, this analysis confirmed that some laboratory testing produced skeletal injury patterns that are seen in comparable theater UBB events.

P3a from a passive visual stimulus task.

OBJECTIVE: Visual event-related brain potentials (ERPs) were elicited using a 3-stimulus oddball paradigm to assess the P3a with passive stimulus processing. METHODS: Young adults (n=12) were presented with a series of visual stimuli consisting of a solid circle standard stimulus (P=0.76) that was difficult to discriminate from a larger target circle (P=0.12), with a large square distractor stimulus (P=0.12) presented randomly in the series. Subjects were instructed in the passive condition to simply look at the stimuli and in the active condition to press a mouse key only to the target stimulus. ERPs were recorded from 15 scalp electrodes, with the amplitude and latency of the P300 from the distractor and target stimuli assessed. RESULTS: The P3a from the distractor stimulus was similar in amplitude, scalp topography, and peak latency across the passive and active task conditions. The P3b from the target stimulus demonstrated much smaller amplitude, highly altered scalp topography, and longer latency for the passive compared to active task conditions. CONCLUSIONS: The P3a can be obtained with visual stimuli in the 3-stimulus paradigm under passive viewing conditions. Theoretical implications and clinical applications are discussed.

Hemispheric differences for visual matrix processing: stimulus size and spatial frequency effects.

Hemispheric processing differences were assessed by presenting square matrices that varied in size and the number of filled-in cells. Subjects judged whether the matrix contained an even or odd number of filled cells. Experiment 1 employed relatively small matrix sizes (2 x 2, 3 x 3, and 4 x 4), and Experiment 2 employed relatively large matrix sizes (4 x 4, 6 x 6, and 8 x 8). Response time was shorter and error rates lower for left visual field/right hemisphere (LVF/RH) presentations compared to right visual field/left hemisphere (RVF/LH) presentations, with the larger matrices demonstrating the strongest visual field/hemispheric effects. Increases in the number of filled cells contributed to increases for the LVF/RH response time advantage only for the larger arrays. Analysis of the data from both studies collapsed across the number of filled cells produced highly consistent LVF/RH advantages for both response time and error rate, with stronger LVF/RH advantages found for the larger matrix sizes of both studies. The findings suggest that visual stimulus spatial frequency is a key determinant of hemispheric processing advantages, but that this factor is constrained by stimulus size variation. Theoretical implications with respect to the hemispheric processing double filtering by frequency model are discussed.

P300 asymmetry in schizophrenia: a meta-analysis.

P300 event-related brain potential (ERP) amplitude is smaller in patients with schizophrenia compared to unaffected controls, but whether left temporal component amplitude is also smaller is debated. The present study employed meta-analytical methods to quantitatively assess previous P300 schizophrenia asymmetry findings. All P300 articles on schizophrenia using an auditory oddball paradigm published before January 2000 were obtained by comprehensive literature searches and cross-referencing for related articles. A total of 19 original articles reporting complete midline electrode data and 11 articles reporting lateral asymmetry electrode data were reviewed, which included different independent conditions that yielded 50 independent data sets. P300 amplitude differences between patients with schizophrenia and control subjects from the midline electrodes yielded effect sizes that differed among recording sites, such that Fz was significantly smaller than Pz, with Cz effect sizes smaller than Pz but larger than Fz. Comparison of P300 amplitude from the lateral data for the T3 and T4 electrodes found no reliable effect size difference when these electrodes were analyzed separately. However, comparison of P300 amplitude effect sizes from the TCP1 was significantly larger than that from the TCP2 when these electrodes were analyzed separately. P300 amplitude is smaller overall in patients with schizophrenia compared to control subjects and differs in its effect size topography across the midline and temporal electrode sites, with the strongest effect sizes obtained for the Pz midline and TCP1 lateral electrodes.

Wavelet analysis of P3a and P3b.

Target/standard discrimination difficulty and the degree of stimulus "novelty" were manipulated systematically in a three-stimulus odd-ball task to assess how these variables affect target and non-target P300 scalp distributions for visual stimuli. Wavelet transformation (WT) analyses were performed on the non-target (P3a) and target (P3b) ERPs to assay how the underlying electroencephalographic (EEG) activity was affected by both the difficulty and novelty factors. When target/standard discrimination was easy, P300 amplitude was higher for the target than the non-target across all electrode sites, and both demonstrated parietal maximums. In contrast, when target/standard discrimination was difficult, non-target amplitude (P3a) was higher and earlier over the frontal/central electrode sites for both levels of novelty, whereas target amplitude (P3b) was greater parietally and occurred later than the non-target components and was generally unaffected by non-target novelty level. The WT analyses indicated that appreciable theta activity was related to the more novel non-target stimuli; primarily target component delta coefficients were affected by the discrimination difficulty variable. The findings suggest that target/standard discrimination difficulty, rather than stimulus novelty, determines P3a generation for visual stimuli but that the underlying theta oscillations are differentially affected by stimulus novelty. WT analysis methods are discussed along with the theoretical and neurophysiological implications of the findings.

P300 and alpha event-related desynchronization (ERD).

In the present study we evaluated the relationships between the P300 event-related potential and event-related desynchronization (ERD) of electroencephalographic alpha activity by simultaneously analyzing P300 as well as 7-10- and 10-14-Hz alpha ERD responses from auditory passive and active oddball conditions. We compared the effects of task (target vs. nontarget) and electrode (Fz, Cz, Pz) on P300 and ERD, and correlated P300 amplitude/latency with ERD maximal amplitude/latency across individuals. The major findings were that P300 as well as slow and fast alpha ERD manifested similar task and electrode effects. P300 preceded ERD and predicted individual variance of both slow and fast alpha ERD. The relationships of P300 with alpha ERD were different for the slow and fast alpha frequencies. These findings indicate that P300 and ERD are related such that slow and fast alpha ERDs are specifically guided or modified by the internal event(s) indexed by P300.

Auditory P3a deficits in male subjects at high risk for alcoholism.

BACKGROUND: Substantial evidence indicates that alcoholism is biologically mediated by a genetic predisposition. As the decreased P300 (P3b) event-related brain potential component does not recover with prolonged abstinence, it is unlikely to be related to drinking history but is more likely to be genetically influenced. This is supported by findings that P3b amplitudes are reduced in subjects at high-risk compared to low-risk for alcoholism. Although there are few studies of P3a in HR subjects, lower P3a amplitudes have been reported with a novel nontarget stimulus paradigm, as well as with a difficult three-stimulus visual paradigm. Using a similar three-tone auditory paradigm in which the discriminability between the target and standard tone is difficult, the P3a component can also be reliably elicited with a rare nontarget perceptually distinct stimulus. This technique was employed in young adult subjects at low-risk and high-risk for alcoholism. METHODS: A total of 17 low-risk and 24 high-risk male subjects were employed as subjects in an auditory paradigm that yielded a large amplitude P3a with a centro-frontal maximum to the nontarget and a robust low amplitude prolonged P3b with a parietal maximum amplitude to the target stimulus. Current source density maps were derived to assess topographic differences between low-risk and high-risk subjects. RESULTS: The high-risk group manifested significantly lower P3a amplitudes than the low-risk group at the frontal electrodes to rare nontarget stimuli. High-risk subjects also demonstrated a more disorganized current source density map for P3a compared to low-risk subjects. CONCLUSIONS: The reduction of P3a in the high-risk group may be due to cortical dysfunction including the frontal and prefrontal cortex. The lower P3a amplitude coupled with more disorganized current source density maps suggest inefficient brain functioning in high-risk subjects.

Tobacco smoking and event-related brain potentials in a Stroop task.

Behavioral and event-related brain potential (ERP) measures were used to assess the effects of tobacco smoking on selective attention. Two groups of abstinent smokers performed a Stroop color-naming task. The display color of a stimulus word determined the correct response, whereas word meaning was irrelevant. Meaning was congruent, neutral, or incongruent with respect to color. After completing two blocks of trials under abstinent conditions, subjects received a 15-min break before performing two more blocks. Subjects in the Smoking group (N=12) smoked two cigarettes during the break. Matched Control subjects (N=12) did not smoke during the break. Typical Stroop effects were found, as reaction time (RT) was shortest to congruent words, intermediate to neutral words, and longest to incongruent words. Overall RT decreased after the break equally for the Smoking and Control groups, whereas the magnitude of the Stroop effect was unchanged for either group. P300 amplitude decreased after the break for the Smoking group but not for the Control group, which implied that smoking rather than practice produced component decline. Error rate and P300 latency did not change after the break for either group. The results suggest that tobacco smoking may decrease the availability of general attentional resources required to evaluate colored word stimuli, whereas the specific stimulus processing mechanisms responsible for the Stroop effect are relatively unaffected.

P300 as a clinical assay: rationale, evaluation, and findings.

Use of the P300 event-related brain potential (ERP) as a clinical assay is reviewed and assessed by comparing its distribution qualities with normative biomedical testing data from published studies. The coefficient of variation statistic was calculated for P300 data and a variety of clinical testing data. P300 amplitude and latency variability was found to be highly comparable and sometimes superior to routinely employed biomedical assays. These results are discussed in terms of how to control inter-group ERP variability and the application of normative P300 data in clinical settings.

Neuroelectric assessment of HIV: EEG, ERP, and viral load.

The effects of the human immunodeficiency virus (HIV) infection on the central nervous system function were studied with electroencephalographic (EEG) and auditory event-related brain potentials (EPRs) in patients infected with HIV and unaffected young adult control subjects (n=10/group). All subjects were assessed once every 15 min for four trial blocks at the same time of day to assess EEG/ERP changes with time on task-induced fatigue. Spectral analysis was applied to the pre- and post-stimulus EEG segments. ERP values were evaluated with respect to group differences for component amplitude and latency measures. Spectral analysis demonstrated that HIV patients evinced greater pre-stimulus delta power over frontal areas compared to control subjects, and less post-stimulus spectral power for the delta, theta, and alpha bands over the central/parietal areas. P300 amplitude was smaller, and latency was marginally longer for the HIV patients compared to control subjects. P300 latency correlated positively with increases in the patient HIV viral load. Time-on-task generally did not affect EEG or ERP measures for either group other than contributing to an overall decrease in neuroelectric responsivity. Group spectral power effects were consistent with differences in arousal/fatigue level. P300 group differences were consistent with declines in cognitive capability, and P300 latency increased with increased viral load. HIV infection negatively affected central nervous system function as measured by EEG and cognitive ERPs in a manner that suggests decreased arousal and increased fatigue in HIV patients.

Auditory P3a assessment of male alcoholics.

BACKGROUND: P3a amplitude differences between alcoholic and control groups have not been well defined. Because event-related potential (ERP) differences between these groups appear to be influenced by task difficulty, the present study employed a new auditory ERP paradigm, in which target/standard tone discriminability was difficult, with infrequent nontarget stimuli used to elicit the P3a. METHODS: A total of n = 27 male alcoholics and n = 25 male controls were assessed using a three-tone discrimination paradigm, in which the discriminability between the target and standard was difficult, with easily discriminable infrequent nontarget tones also presented. A P3a component with a centro-frontal maximum to the rare nontargets and a P3b with a parietal maximum amplitude to the target stimulus were obtained. Current Source Density (CSD) maps were derived from the potential data and employed to assay topographical differences between subject groups. RESULTS: Alcoholics produced smaller P3a amplitudes than control subjects to the rare nontargets with no peak latency differences observed. The most prominent current sources are apparent more anteriorly for the nontarget compared to the target stimulus in both groups. There were more sources and sinks in the alcoholics than in the control subjects for P3a. A bootstrap analysis method showed that P3a CSD maps evinced distinct topographic distributions between alcoholics and control subjects in all brain regions. CONCLUSIONS: The lower P3a amplitude and weaker sources in alcoholics coupled with less topographic specificity in their CSD maps, suggests disorganized inefficient brain functioning. This global electrophysiological pattern suggests cortical disinhibition perhaps reflecting underlying CNS hyperexcitability in alcoholics.

The P300 brain potential is reduced in smokers.

RATIONALE: Tobacco smoking is the most prevalent type of substance abuse, yet its biobehavioral etiology is little understood. Identification of differences between smokers and non-smokers on basic characteristics of neurocognitive functioning may help to elucidate the mechanisms of tobacco dependence. OBJECTIVES: This study assessed the relationship between smoking status and the P300 component of event-related potential (ERP) while controlling for potential confounders such as alcoholism, drug abuse, and psychopathology. METHODS: The ERP responses elicited by a visual oddball task were measured at the mid-parietal site in 905 current smokers, 463 ex-smokers, and 979 never smokers. RESULTS: P300 amplitude was significantly lower in current cigarette smokers compared to never-smokers. Ex-smokers did not differ significantly from never-smokers. P300 reduction was also associated with alcoholism, drug dependence, and family density of alcoholism. However, after controlling for smoking, only family density of alcoholism remained a significant predictor of P300 amplitude. CONCLUSIONS: The results indicate a significant effect of smoking status on P300 amplitude which is additive to family history of alcoholism and suggest that either (1) long-term tobacco smoking may produce a reversible change in brain function, or (2) reduced P300 may be a marker of risk for nicotine dependence.

Olfactory event-related potentials and aging: normative data.

Unlike the clinical usages of evoked potentials (e.g. brain stem auditory evoked potentials for the assessment of auditory function), normative data for the olfactory event-related potential (OERP) have been unavailable. The principal objective was to establish normative data across the human life span for OERPs with a given set of parameters. Participants were 140 persons from seven age groups (16-19, 20-29, 30-39, 40-49, 50-59, 60-69 and 70-79 years of age), with equal numbers of males and females, screened for nasal health and dementia. The odor stimulus was amyl acetate, presented at nasal temperature in a humidified airstream delivered by an air-dilution olfactometer at a constant flow rate, using a 60-s inter-stimulus interval. OERPs were recorded at Fz, Cz, and Pz electrode sites, amplified and averaged over trials. Amplitudes of the N1/P2 and P3 and latencies of the P2 and P3 were analyzed. Processing speed decreased at a constant rate over decades for the sensory (P2 latency) as well as cognitive (P3 latency) components. Decline in amplitude over decades was also apparent. Normative data will be useful in research on olfactory function and in clinical assessment of olfactory functional status.

P300 and blink instructions.

OBJECTIVES: The effects of instructions to refrain from blinking on the P300 event-related brain potential (ERP) from auditory and visual stimuli were assessed. METHODS: An oddball paradigm was employed in which young adult subjects (n = 20) silently counted the infrequent target stimuli and were given either no instructions or told "do not blink" in different conditions, with auditory and visual stimulus tasks employed for all subjects. ERPs were recorded from the midline electrodes, with amplitude and latency of the P300 and other components obtained. RESULTS: P300 amplitude for both modalities and target/standard stimulus conditions was smaller and visual peak latency longer in the "do not blink" condition. Blink instructions did not directly affect the other components. CONCLUSIONS: Instructions to refrain from blinking can decrease P300 amplitude and can increase peak latency.

On P300 measurement stability: habituation, intra-trial block variation, and ultradian rhythms.

P300 event-related brain potentials (ERPs) were elicited using a simple discrimination task in which participants discriminated two different equiprobable visual stimuli with button-press responses (n = 20). A total of ten trial blocks were presented at 10-min intervals. P300 amplitude declined significantly, but peak latency did not change reliably across trial blocks. P300 amplitude demonstrated a reliable cyclical fluctuation across trial blocks, although P300 latency did not. Intra-trial block ERP variability was assessed by computing the correlation coefficients between the target and standard stimuli for amplitude and latency measures across participants within each trial block. P300 amplitude correlations were weakest at the Fz electrode, more strongly associated at Cz, and were most strongly correlated at Pz across trial blocks. P300 latency correlations were somewhat weaker and similar in strength across electrodes sites. The correlational patterns for both P300 amplitude and latency demonstrated reliable cyclical variation. The N100 component produced strong and consistent correlations for both amplitude and latency, whereas the P200 and N200 component measures evinced cyclical correlational patterns similar to the P300 across trial blocks. These results suggest that the stability of P300 and other component measures can vary appreciably within and across trial blocks in a manner that reflects ultradian variation in arousal level.

Comparison of P300 from passive and active tasks for auditory and visual stimuli.

The P300 event-related brain potential (ERP) was elicited with a stimulus-sequence paradigm for auditory and visual stimuli in separate active and passive task response conditions. Auditory stimuli in the passive task yielded P300 waveforms similar to those obtained from the active task condition. Visual stimuli in the passive task yielded much smaller P300 waveforms that were not morphologically consistent with those from the active task. The results suggest that auditory stimuli produce more robust P300 components than visual stimuli in passive task situations.

Olfactory event-related potentials in young and elderly adults: evaluation of tracking task versus eyes open/closed recording.

The purpose of the present study was to evaluate olfactory event-related potentials (OERPs) elicited by amyl acetate from subjects performing a visuomotor tracking task compared with the no-task conditions of eyes open and eyes closed. Task condition did not produce any reliable effects for any amplitude measure. Task type weakly influenced only P2 latency. Elder adults evinced smaller P2 and N1/P2 amplitudes and longer N1 and P2 latencies than young adults. The results suggest that tracking task performance is not necessary to obtain robust OERPs from normal subjects of a wide age range.

P300 habituation patterns: individual differences from ultradian rhythms.

Event-related brain potentials (ERPs) were elicited with auditory stimuli from normal subjects every 10 min. for 10 successive trial blocks to assess ultradian influences on the P300 component. Based on a theoretical analysis of the underlying relationship between background EEG activity and P300 variability, subjects were divided into two groups according to whether P300 amplitude increased or decreased initially over trial blocks. P300 amplitude habituated across trial blocks. P300 latency produced systematic cyclical variation with approximately a 90-min. period that was opposite in phase for the different subject groups. The findings suggest that ultradian rhythms contribute to P300 latency variability. The implications of the results for P300 in applied settings are discussed.

Normal aging and odor intensity effects on the olfactory event-related potential.

Olfactory event-related potentials (OERPs) were recorded in 14 young and 14 older adults, with odor strength of isoamyl acetate manipulated to assess olfactory stimulus intensity. Young participants produced significantly larger N1/P2, N2/P3 amplitudes and shorter N1, P2 and N2 latencies than older participants. Medium- and high-odor concentrations elicited significantly shorter P2 and N2 latencies than the lowest concentration for both age groups. Odor concentration appears to affect the speed of olfactory stimulus information processing regardless of age.