ABSTRACT
OBJECTIVE: To search for morphologic nuclear features in the epithelial lining of odontogenic keratocysts to differentiate simple from Gorlin syndrome cysts. STUDY DESIGN: Five cases of syndrome-associated keratocysts and five of simple ones were studied. Thirty nuclei from the epithelial basal layer for each case were analyzed by the shape analytical morphometry (SAM) software system to quantitatively evaluate nuclear dimensions (area, perimeter, diameter), contour irregularities and nuclear shape asymmetries. Results were subjected to Student's t test and cluster analysis. RESULTS: Values of nuclear dimensions were very close in both groups of keratocysts, without any significant statistical differences. The variables related to nuclear profile irregularities, as well as those describing nuclear asymmetry, showed significantly higher values (P < .001) in syndromic cysts. Cluster analysis produced two different clusters by using variables related to nuclear contour irregularities. CONCLUSION: Preliminary results indicate the existence of nuclear morphologic differences between simple and syndromic cysts.
Subject(s)
Basal Cell Nevus Syndrome/genetics , Cell Nucleus/ultrastructure , Adult , Aged , Basal Cell Nevus Syndrome/pathology , Cell Nucleus/genetics , Cluster Analysis , Diagnosis, Differential , Disease Progression , Female , Humans , Male , Middle Aged , Mutation , Odontogenic Cysts/genetics , Odontogenic Cysts/ultrastructureABSTRACT
A study was conducted on a new acid peroxygen system based disinfectant (Virkon), in order to assess its in vitro efficacy. The chemical was tested on different bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli), spores (Bacillus subtilis) and on the Hepatitis B surface antigen (HBsAg), and compared in its activity with phenol and glutaraldehyde (calculation of the 'phenol coefficient' and the 'glutaraldehyde coefficient'). The constancy of speed of disinfection, the coefficient of concentration, the minimal inhibitory concentration (MIC) were also determined, and the destruction of the HBsAg antigenic activity was studied using an ELISA kit. The sporicidal efficacy of Virkon was assessed by cultivating spores in agar nutrient after contact with different dilutions of the disinfectant. The results of the tests showed that Virkon has a high concentration coefficient (mean value of k: 0.374/min) and a wide range of action. The low MIC demonstrates how little concentrations of Virkon can inactivate all studied bacteria. The disinfectant was also able to destroy the hepatitis B surface antigen, and it demonstrated good activity against spores, especially if used in physiologic solution. These characteristics, coupled with the absence of initiation or toxic effects on animals showed by other studies, make wide fields of application for the new disinfectant foreseeable.
Subject(s)
Bacteria/drug effects , Disinfectants/pharmacology , Hepatitis B Surface Antigens/drug effects , Peroxides/pharmacology , Sulfuric Acids/pharmacology , Animals , Bacillus subtilis/drug effects , Disinfectants/administration & dosage , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical , Enzyme-Linked Immunosorbent Assay , Escherichia coli/drug effects , Glutaral/pharmacology , Hepatitis B virus/drug effects , Peroxides/administration & dosage , Phenol , Phenols/pharmacology , Pseudomonas aeruginosa/drug effects , Spores, Bacterial/drug effects , Staphylococcus aureus/drug effects , Sulfuric Acids/administration & dosage , Time FactorsABSTRACT
Yield and collection efficiency are two important and strictly related aspects of an apheresis system. We have examined the various formulas, dwelling upon a few particular aspects of the collection of platelets, granulocytes and stem cells.
