Comparison of the characteristics of chinchilla epidydimal semen after collection, storage at 5°C and cryopreservation.

The aim of the study was to compare the characteristics of chinchilla epididymal sperm: fresh, stored at liquid state and cryopreserved. Epididymal spermatozoa obtained from 11 males were assessed for subjective motility, concentration, motility parameters measured by CASA, viability, morphology, membrane integrity, acrosome integrity, mitochondrial potential, lipid peroxidation, chromatin structure, apoptotic changes and capacitation. Then half of the spermatozoa were stored at 5°C for 30 hr, and the second half was cryopreserved. After storage and thawing the same parameters as in fresh semen were assessed. Fresh semen showed good quality, with low levels of lipid peroxidation, chromatin fragmentation and capacitation. CASA evaluation showed significantly lower values for MOT, PMOT, RAPID, VCL, VAP and VSL after both storage at liquid state and cryopreservation (p < 0.05). Cold storage did not induce membrane and acrosome damage (p > 0.05), conversely to cryopreservation (p < 0.05). After storage, there was a drop in high mitochondrial potential in live cells (p < 0.05) and an increase in the percentage of non-apoptotic, capacitated cells (p < 0.05). These changes were not seen after cryopreservation (p > 0.05). Lipid peroxidation in live cells and chromatin structure remained unchanged both after storage and cryopreservation (p > 0.05). The study showed that examined methods of semen preservation exerted different patterns of changes in spermatozoa and that sperm quality after both of them allowed for further use of preserved spermatozoa in artificial reproductive techniques.

Infrared technology for estrous detection in Chinchilla lanigera.

Thermography is one of the most modern methods for non-invasive monitoring of animal behavior, health status and physiological state. The sparcity of scientific reports related to this subject in fur animals prompted the use of thermography in Chinchilla lanigera. The aim of the study was to evaluate the use of a thermal imaging camera to diagnose or confirm estrus in Chinchilla lanigera. The subjects included 100 female chinchillas from three different breeds, selected during ovulation after parturition, which were evaluated twice: measurements were taken over three successive days starting from the 2nd day post partum and again through the same time period 6 weeks later when the females were ready to mate. A picture of the external genital area was taken with a FLIR C2 thermal imaging camera and the temperature was determined and recorded from the selected area using the FLIR TOOLS computer program. There were distinct differences between chinchillas with and without a confirmed estrus. Chinchillas in estrus had ROI temperatures that were greater (2.33 °C) than non-estrous females. During estrus, the temperature of the external genital area clearly increases, which could be due to genital hyperemia. This fact can be used for non-invasive estrous diagnosis, which is especially important because of the absence of typical estrous behavior in this species. Monitoring the greater surface temperature of the vulval area allows determination of the optimal time to place a female with a male and expect effective copulation.