ABSTRACT
Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0-95.2%) and 94.8% (95% CI: 93.1-96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.
Subject(s)
Cattle Diseases/diagnosis , Enzyme-Linked Immunosorbent Assay/veterinary , Foot-and-Mouth Disease/diagnosis , Viral Nonstructural Proteins/metabolism , Animals , Antibodies, Viral/blood , Cattle , Cattle Diseases/blood , Cattle Diseases/virology , Enzyme-Linked Immunosorbent Assay/standards , Foot-and-Mouth Disease/blood , Foot-and-Mouth Disease/virology , Foot-and-Mouth Disease Virus , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
Q fever is an important zoonotic disease caused by infection with the bacterium Coxiella burnetii. Veterinary diagnostic laboratories, including the Veterinary Laboratories Agency (VLA) in England and Wales, have traditionally relied on the complement fixation test (CFT) for serological diagnosis. However, Q fever has assumed greater significance in recent years following several large human outbreaks linked to exposure to infected ruminants and it is essential that more reliable tests are introduced to detect the presence of C. burnetii infection in animals. The objective of the current study was to evaluate the performance of 3 commercially available enzyme-linked immunosorbent assays (ELISAs) for detection of antibodies to C. burnetii and to compare the findings with the CFT using a sample panel of 548 sera from sheep, goats, and cattle, including animals of known disease status. The statistical analysis using TAGS (test accuracy in the absence of a gold standard) software and receiver operating characteristic techniques demonstrated that the 3 ELISAs all showed improved sensitivity over the CFT. The test based on ovine antigen demonstrated the best overall performance and therefore, the VLA has adopted this test for routine use.
