[Experimental studying of the possible pharmacological defense of muscular flap tissues from ischemic and reperfusion injury during its free transplantation]. / Eksperimental'noe izuchenie vozmozhnosti farmakologicheskoi zashchity tkanei myshechnogo loskuta ot ishemicheskogo i reperfuzionnogo povrezhdeniia pri osushchestvlenii ego svobodnoi peresadki.

In experiment on muscle flaps of 20 rabbits the peculiarities of their tissues damage in the ischemia period, and also due to the oxygen-dependent processes activation (peroxidal and free radical oxidation of a membrane phospholipids) in period of its reperfusion were studied. Preliminary introduction of a lipin, unitiol and ascorbic acid mixture in the flap arterial channel had promoted the reduction of damage severity in the translocated flap cells biomembranes due to ischemia and reperfusion, causing inhibition of the lipids peroxidal oxidation processes, rising of the flap tissues antioxidant defense, the cell and subcell membranes stabilization which preserves the vessels endothelium integrity. This showed a promising future for application of these preparations in a free transplantation of compound tissue complexes.

[Experimental substantiation of a two-staged method of microsurgical transplantation of the complex composite flaps]. / Eksperimental'noe obosnovanie dvukhétapnogo metoda mikrokhirurgicheskoi peresadki slozhno-sostavnykh loskutov.

The authors have proposed two-staged flap transplantation, consisting of flap mobilization on vascular pedicle with subsequent its transplantation in several days. In experiment on muscular flaps, obtained from regio epigastrica of 30 rabbits, it was established that optimal time for the second stage of operation conduction may be end of the second--beginning of the third day after the transplant mobilization.

[Antibodies to E. coli and circulating immune complexes in the bile and blood serum of patients with suppurative cholangitis]. / Antitela k E. coli i tsirkuliruiushchie immunnye kompleksy v zhelche i syvorotke krovi bol'nykh gnoinym kholangitom.

It has been found out for the first time that in 55% of cases presenting with purulent cholangitis, there are antibodies to a clinical strain of E. coli in the bile taken intraoperatively from major bile duct whereas museum specimens of E. coli strain are free from the above antibodies. Immune complexes were identified in the bile of all examined patients (0.316 +/- 0.085 arbitrary units). In the blood serum of patients with purulent cholangitis, antibodies to the clinical strain E. coli are not detectable, while there are great counts of circulating immune complexes (0.928 +/- 0.242 arbitrary units). Based on the findings obtained a conclusion was reached about relative independence of local immunity of the biliary tract.

[State of lipid peroxidation and blood antioxidant system in patients with occlusive arterial diseases of the limbs]. / Stan perekysnoho okyslennia lipidiv i antyoksydantnoï systemy krovi u patsiientiv z okliuzuiuchymy zakhvoriuvanniamy arterii kintsivok.

In 95 patients with atherosclerotic occlusion of aorto-iliac and femoro-popliteal arterial segments and II-IV grade of ischemia (according to Fontaine) the concentration of malonic dialdehyde and general antioxidant activity of the blood plasma in ischemia period of time and in various terms of revascularization period were determined. The processes rising of peroxide clearance of lipids (PCL) in injured limb and lowering of antioxidant (AO) activity of blood plasma was discovered. In the revascularization period further significant activation of PCL processes (maximally during the first day) and gradual rising of AO activity of blood plasma (maximally on the 3-5th day) was revealed. The magnitude of these processes depended on the tissue ischemia extension and its severity.