ABSTRACT
We found in experiments involving the use of a biopreparation lymphotilin that its administration was followed by a decrease in proliferative activity of cultured tumor cells and a longer survival of mice bearing transplantable leukemia. An intensified intercalation of ethidium bromide in nucleic acids of tumor cells in lymphotilin culture points to the drug activity on nuclear level. Tumor cell inhibition by lymphotilin holds much promise for the practice of hematology.
Subject(s)
Anticarcinogenic Agents/pharmacology , Antineoplastic Agents/pharmacology , Leukemia, Experimental/drug therapy , Leukocytes/drug effects , Animals , Cell Division/drug effects , Cells, Cultured , Drug Interactions , Enzyme Inhibitors/pharmacology , Ethidium/pharmacology , Mice , Nucleic Acids/drug effects , Time Factors , Tumor Cells, CulturedABSTRACT
The possibility of determining the coefficients of diffusion in solution by the transport of solutes through porous polymeric membrane was studied. Reliable and reproducible results can be obtained by using nucleoporous filters with cylindrical pores. The method enables the selective determining of the diffusion coefficients of solutes being in complex mixtures, which is of special interest for biochemical research. The possibilities of the method are illustrated on the pattern of some globular proteins, polyethylene glycols and proteolytic enzymes.
Subject(s)
Diffusion , Membranes, Artificial , Mathematics , PermeabilityABSTRACT
The sedimentation-biochemical method of determination of enzymes' sedimentation coefficients and estimation of their molecular masses based on the Tiselius' transport technique is discussed. The method permits to determine the sedimentation coefficients of enzyme in the starting mixtures without their preliminary purification. The use of different substrats permits to carry out the studying of several enzymes simultaneously. The frameworks of the method are discussed.
