Ciprofloxacin Electrochemical Sensor Using Copper-Iron Mixed Metal Oxides Nanoparticles/Reduced Graphene Oxide Composite.

The harmful effects of antibiotic proliferation on the environment and its persistent nature are urgent global problems. Ciprofloxacin (CIP) is a fluoroquinolone-class antibiotic agent used widely to treat pathogen-related diseases in humans and animals. Its excretion into surface water causes antibiotic resistance in microbes, resulting in difficult-to-treat or untreatable infectious diseases. This study developed a simple and efficient electrochemical sensor to detect CIP. Hydrothermal chemistry was utilized to synthesize an electrophotocatalytic composite of copper-iron mixed metal oxides (CIMMO) on reduced graphene oxide (rGO) (CIMMO/rGO). The composite was employed in an electrochemical sensor and exhibited outstanding performance in detecting CIP. The sensor was operated in differential pulse voltammetry (DPV) mode under light source illumination. The sensor yielded a linear response in the concentration range of 0.75 × 10-9-1.0 × 10-7 mol L-1 CIP and showed a limit of detection (LOD) of 4.74 × 10-10 mol L-1. The excellent sensing performance of the composite is attributable to the synergic effects between CIMMO nanoparticles and rGO, which facilitate photoinduced electron-hole separation and assist in the indirect electrochemical reactions/interactions with CIP.

Bioponic systems with biochar: Insights into nutrient recovery, heavy metal reduction, and microbial interactions in digestate-based bioponics.

Bioponics is a nutrient-recovery technology that transforms nutrient-rich organic waste into plant biomass/bioproducts. Integrating biochar with digestate from anaerobic wastewater treatment process can improve resource recovery while mitigating heavy metal contamination. The overarching goal of this study was to investigate the application of biochar in digestate-based bioponics, focusing on its efficacy in nutrient recovery and heavy metal removal, while also exploring the microbial community dynamics. In this study, biochar was applied at 50 % w/w with 500 g dry weight of digestate during two 28-day crop cycles (uncontrolled pH and pH 5.5) using white stem pak choi (Brassica rapa var. chinensis) as a model crop. The results showed that the digestate provided sufficient phosphorus and nitrogen, supporting plant growth. Biochar amendment improved plant yield and phosphate solubilization and reduced nitrogen loss, especially at the pH 5.5. Furthermore, biochar reduced the heavy metal accumulation in plants, while concentrating these metals in the residual sludge. However, owing to potential non-carcinogenic and carcinogenic health risks, it is still not recommended to directly consume plants cultivated in digestate-based bioponic systems. Additionally, biochar amendment exhibited pronounced impact on the microbial community, promoting microbes responsible for nutrient solubilization and cycling (e.g., Tetrasphaera, Herpetosiphon, Hyphomicrobium, and Pseudorhodoplanes) and heavy metal stabilization (e.g., Leptolinea, Fonticella, Romboutsia, and Desulfurispora) in both the residual sludge and plants. Overall, the addition of biochar enhanced the microbial community and facilitated the metal stabilization and the cycling of nutrients within both residual sludge and root systems, thereby improving the overall efficiency of the bioponics.

Effects of vermicompost leachate on nitrogen, phosphorus, and microbiome in a food waste bioponic system.

Food waste is rich in nutrients, such as nitrogen and phosphorus, and can be integrated with bioponics, a closed-loop agricultural system that combines hydroponics with biological nutrient recovery. Vermicompost leachate (VCL) supplementation has been shown to improve the co-composting of organic waste (i.e., compost quality) and the biodegradation of organic compounds. Thus, VCL has high potential for enhancing nutrient availability in bioponics from food waste. However, the understanding of nitrogen and phosphorus availability in food waste-based bioponics is limited, both with and without VCL. In this study, food waste derived from cafeteria vegetable waste was used as the substrate (500 g dry wt./system) in bioponics to grow lettuce (Lactuca sativa L.) for two consecutive cycles (35 days/cycle) without substrate replacement. VCL was applied weekly (1-5% v/v) and compared to the control without VCL. The results showed that the food waste in bioponics provided nitrogen and phosphorus for plant growth (15.5-65.8 g/lettuce head). Organic-degrading and nutrient-transforming bacteria (Hydrogenispora, Clostridium_sensu_stricto_1, Ruminiclostridium_1, Cellvibrio, Thauera, Hydrogenophaga, and Bacillus) were predominantly found in plant roots and residual food waste. VCL addition significantly increased nitrate, phosphate, and chemical oxygen demand levels in bioponics, owing to the nutrients in VCL and the enhancement of keystone microorganisms responsible for organic degradation and nutrient cycling (e.g., Ellin6067, Actinomyces, and Pirellula). These findings suggest that nitrogen, phosphorus, and organic carbon concentrations in an ecosystem of nutrient-transforming and organic-degrading microbes are key in managing nutrient recovery from food waste in bioponics.

Electrochemical Sensor Based on a Composite of Reduced Graphene Oxide and Molecularly Imprinted Copolymer of Polyaniline-Poly(o-phenylenediamine) for Ciprofloxacin Determination: Fabrication, Characterization, and Performance Evaluation.

Contamination of antibiotics in water is a major cause of antibiotic resistance (ABR) in pathogens that endangers human health and food security worldwide. Ciprofloxacin (CIP) is a synthetic fluoroquinolone (FQ) antibiotic and is reportedly present in surface water at a concentration exceeding the ecotoxicological predicted no-effect concentration in some areas. This study fabricated a CIP sensor using an electropolymerized molecularly imprinted polymer (MIP) of polyaniline (PANI) and poly(o-phenylenediamine) (o-PDA) with CIP recognition sites. The MIP was coated on a reduced graphene oxide (rGO)-modified glassy carbon electrode (rGO/GCE) and operated under a differential pulse voltammetry (DPV) mode for CIP detection. The sensor exhibited an excellent response from 1.0 × 10-9 to 5.0 × 10-7 mol L-1 CIP, showing a sensor detection limit and sensitivity of 5.28 × 10-11 mol L-1 and 5.78 µA mol-1 L, respectively. The sensor's sensitivity for CIP was 1.5 times higher than that of the other tested antibiotics, including enrofloxacin (ENR), ofloxacin (OFX), sulfamethoxazole (SMZ), and piperacillin sodium salt (PIP). The reproducibility and reusability of the sensor devices were also studied.

Characteristics of Gases Emitted from Chicken Manure Wastewater and Potential Effects on Human Health.

Hazardous and odorous gas emissions from chicken manure wastewater are a public preoccupation. Odor composition measurements are crucial steps to provide appropriate air pollution control measures and design strategies to minimize the human health impact. In this study, chicken manure wastewater samples were extensively analyzed in a closed system by a portable handheld gas detector to characterize the odor and chemical compositions. A portable detector was calibrated prior to sampling. The total number of 153 chemical compounds found in the chicken manure wastewater samples had various olfactory characteristics, including strong, pleasant, and odorless smells. Depending on the route of exposure, most of these chemical compounds could cause human health effects, such as irritation, burns, and severe symptoms including carcinogenic.

Effects of Cu and Zn contamination on chicken manure-based bioponics: Nitrogen recovery, bioaccumulation, microbial community, and health risk assessment.

In bioponics, although chicken manure is an efficient substrate for vegetable production and nitrogen recovery, it is often contaminated with high Cu and Zn levels, which could potentially cause bioaccumulation in plants and pose health risks. The objectives of this study were to assess nitrogen recovery in lettuce- and pak choi-based bioponics with Cu (50-150 mg/kg) and Zn (200-600 mg/kg) supplementation, as well as their bioaccumulation in plants, root microbial community, and health risk assessment. The supplementation of Cu and Zn did not affect nitrogen concentrations and plant growth (p > 0.05) but reduced nitrogen use efficiency. Pak choi showed higher Cu and Zn bioconcentration factors than lettuce. Bacterial genera Ruminiclostridium and WD2101_soil_group in lettuce roots and Mesorhizobium in pak choi roots from Cu and Zn supplemented conditions were significantly higher (p < 0.05) than controls, suggesting microbial biomarkers in plant roots from Cu and Zn exposure bioponics depended on plant type. Health risk assessment herein revealed that consumption of bioponic vegetables with Cu and Zn contamination does not pose long-term health risks (hazard index <1) to children or adults, according to the US EPA. This study suggested that vegetable produced from chicken manure-based bioponics has low health risk in terms of Cu and Zn bioaccumulation and could be applied in commercial-scale system for nutrient recovery from organic waste to vegetable production; however, health risk from other heavy metals and xenobiotic compounds must be addressed.

Chicken manure-based bioponics: Effects of acetic acid supplementation on nitrogen and phosphorus recoveries and microbial communities.

Bioponics has the potential to recover nutrients from organic waste streams, such as chicken manure and digestate with high volatile fatty acid (VFA) contents through crop production. Acetic acid, a dominant VFA, was supplemented weekly (0, 500, 1000, and 1500 mg/L) in a chicken manure-based bioponic system, and its effect on the performance of bioponics (e.g., plant yield and nitrogen and phosphorus availabilities) was examined. Microbial communities were analyzed using 16S rRNA gene sequencing, and the functional gene abundances were predicted using PICRUSt. Although acetic acid negatively affected plant yield, no significant difference (p > 0.05) was noted in the average nitrogen or phosphorus concentration. In terms of nutrient recovery, the bioponic systems still functioned well, although higher concentrations of acetic acid decreased plant yield and altered the bacterial communities in plant roots and chicken manure sediments. These data suggest that an acetic acid concentration of < 500 mg/L or a longer loading interval is recommended for the effective operation of chicken manure and digestate-based bioponics.

Bioponic system for nitrogen and phosphorus recovery from chicken manure: Evaluation of manure loading and microbial communities.

Bioponics integrates the biological treatment of nutrient-rich waste streams with hydroponics. However, there are several challenges of bioponics, especially nutrient availability and qualities, which affect plant yield. In this study, chicken manure based-nutrient film technique bioponics was examined at manure loadings of 200, 300, and 400 g dry wt. per bioponic system (total of 18 plants). Bioponics effectively released nitrogen and phosphorus (total ammonia nitrogen of 5.8-8.0 mgN/L, nitrate of 7.0-11.2 mgN/L, and phosphate of 48.7-74.2 mgP/L) for efficient growth of lettuce (Lactuca sativa; total yield of 1208-2030 g wet wt. per 18 plants). Nitrogen and phosphorus use efficiencies were 35.1-41.8% and 6.8-8.0%, respectively, and were comparable to aquaponics. Next-generation sequencing was used to examine the microbial communities in digested chicken manure and plant roots in bioponics. Results showed that several microbial genera were associated with organic degradation (e.g., Nocardiopsis spp., Cellvibrio spp.), nitrification (Nitrospira spp.), phosphorus solubilization, and plant growth promotion (e.g., WD2101_soil_group, and Bacillus spp.). Nocardiopsis spp., Romboutsia spp. and Saccharomonospora spp. were found at high abundances and a high degree of co-occurrences among the microbiota, suggesting that the microbial organic decomposition to nitrogen and phosphorus release could be the key factors to achieve better nutrient recovery in bioponics.

Performance of novel constructed wetlands for treating solar septic tank effluent.

To improve treatment performance of the solar septic tank technology, novel constructed wetland systems have been proposed as an effective post-treatment system. This study aimed to investigate the treatment performance of the multi-soil layer based constructed wetland (MSL-CW) and comparing with the modified constructed wetland (mCW) for treating solar septic tank effluent in long-term operation. Pilot-scale MSL-CW and mCW units were operated in parallel under the same conditions during the period of 2016-2019. Removal efficiencies of TCOD, SCOD and TBOD in the MSL-CW were not significantly different (p < 0.05) from those of the mCW unit, which were 70-72%, 63-68% and 78-82%, respectively. The removal efficiencies of TSS, TKN, NH4-N and TP were found in the same magnitude in both units. The total coliform and E.coli counts in the effluent of MSL-CW and mCW units were reduced from 105 MPN/100 mL to be lower than 103 MPN/100 mL. These long-term operational results demonstrated that the effluent from the MSL-CW and mCW units could meet the global standards of non-sewered sanitation systems and the WHO guidelines. The effects of seasonal variations and plant harvesting on the monthly treatment performance are discussed in this study.

Comparative removal of two antibiotic resistant bacteria and genes by the simultaneous use of chlorine and UV irradiation (UV/chlorine): Influence of free radicals on gene degradation.

The research aimed to remove antibiotic resistance by the simultaneous use of UV irradiation and chlorine (UV/chlorine). The inactivations of tetracycline resistant bacteria (TRB) during chlorination, UV irradiation, and UV/chlorine was investigated and compared with those of amoxicillin resistant bacteria (AmRB). Similar examination was also conducted for comparing the removals of their resistant genes (i.e., tetM and blaTem). The removals of antibiotic resistance highly depended on chlorine doses and UV intensities. The sufficient chlorine dose (20 mg.L-1) in the chlorination and the UV/chlorine completely inactivated TRB and AmRB (>7.3 log), while the UV irradiation could not achieve the complete disinfection. Microorganisms resistant to different antibiotics exhibit different susceptibility to the disinfection processes. The removals of antibiotic resistant genes (i.e., tetM and blaTem) were more difficult than those of TRB and AmRB. The UV/chlorine was the greatest process for tetM and blaTem removals, followed by chlorination and UV irradiation, respectively. Chlorination decreased the tetM and blaTem by 0.40-1.45 log and 1.04-2.45 log, respectively. The blaTem gene was highly reactive to chlorine, compared with tetM. The UV irradiation caused the tetM and blaTem reductions by 0.32-0.91 log and 0.59-0.96 log, respectively. The UV/chlorine improved the tetM and blaTem removals by 0.98-3.20 log and 1.28-3.36 log, respectively. The •OH contributed to the fraction of tetM and blaTem removals by 48% and 19%, respectively. The effect of reactive chlorine species on the tetM and blaTem removals was minor. The pseudo 1st-order kinetic constants (k') for tetM and blaTem removals by the UV/chlorine were highest. The •OH enhanced the k' values by 120% and 20% for the tetM and blaTem removals, respectively. The study showed the potential use of UV/chlorine for controlling antibiotic resistance.

Performance evaluation of novel attached-growth high rate algal pond system with additional artificial illumination for wastewater treatment and nutrient recovery.

Domestic wastewater containing a high proportion of organic matter and nutrients is a serious pollution problem in developing countries. This study aimed to evaluate the performance of a novel attached-growth high rate algal pond (AG-HRAP) employing attached-growth media and artificial light sources for treating domestic wastewater and enhancing nutrient recovery. Light intensities in the range of 40-180 µmol/m2/s were used in the AG-HRAPs. The experimental results showed that the highest chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) removal efficiencies of 88, 62 and 69%, respectively, were found at the hydraulic retention time (HRT) of 15 days and the average light intensity of 180 µmol/m2/s. Moreover, the effluent COD concentrations could meet Thailand's national discharge standard. The highest biomass and protein productivities of 54 ± 4 and 37 ± 8 g/m2/d, respectively, were found in the AG-HRAPs, which were higher than in previous studies of HRAPs. The Stover-Kincannon kinetic values for COD, TN and TP removals of the AG-HRAPs (R2 = 0.9) were higher than those of the conventional systems. Additionally, the novel AG-HRAP system could provide a highly cost-effective operation when compared to other microalgal systems.

Nouveau design solar septic tank: Reinvented toilet technology for sanitation 4.0.

The up-flow solar septic tank (UTST) and multi-soil layering (MSL) system has been developed and proposed as "Nouveau Design Solar Septic Tank". The objective of this study was to verify functionality of the integrated UTST and MSL system for treatment of toilet wastewater (or black water) under actual conditions over a year at the Asian Institute of Technology campus, Pathumthani province, central Thailand. During the operation period which involved fluctuating flow rates, ambient temperatures and black water characteristics, the UTST unit yielded satisfactory performance with the average treatment efficiencies of 92 ± 10% for total chemical oxygen demand (TCOD), 79 ± 10% for soluble chemical oxygen demand (SCOD), 93 ± 9% for total 5-days biochemical oxygen demand (TBOD) and 90 ± 12% for soluble 5-days biochemical oxygen demand (SBOD), respectively, while the MSL unit could remove 95 ± 3%, and 88 ± 15% of total kjeldahl nitrogen (TKN) and total phosphorus (TP), respectively. The effluent TCOD, TBOD, TKN, nitrite (NO2-N), nitrate (NO3-N), ammonia (NH3) and TP concentrations of the integrated UTST and MSL system were 39 ± 27,8 ± 27,5 ± 5 mg/L, 2 ± 2,39 ± 24,8 ± 9,2 ± 5 and 1 ± 1 mg/L, respectively, meeting the ISO requirements. The removal efficiencies of TCOD, SCOD, TBOD and SBOD exhibited positive correlation with the ratios of TBOD/TKN, TBOD/SBOD and TBOD/TP. With high treatment efficiencies and effluent quality meeting the ISO requirements, the nouveau design solar septic tank has been demonstrated as an innovative technology toward the sanitation 4.0 concept and the Sustainable Development Goal no. 6 (SDG6).

Comparative study on attached-growth photobioreactors under blue and red lights for treatment of septic tank effluent.

Attached-growth photobioreactors (AG-PBRs) employing low-cost attached-growth media were applied to treat septic tank effluent which contained abundant organic and nutrient matters as well as pathogenic microorganisms. This study investigated effects of blue and red LED lights on organic, nutrient and pathogenic removals, biomass productivity and compositions of microbial community in the AG-PBR system. The experimental results showed the blue AG-PBR to be more effective in removing chemical oxygen demand (COD), total nitrogen (TN) and ammonia nitrogen (NH3-N) and generating biomass productivity than those of the red AG-PBR (P < 0.05). Mass balance analysis indicated that the TN and total phosphorus (TP) were removed mainly by assimilation into the biomass. The TN removal rates via nitrification and denitrification processes in the blue AG-PBR were found to be higher than that of the red AG-PBR, corresponding to the observed results of bacterial biomass and abundances of nitrifying and denitrifying bacterial species in the treatment systems. The maximal areal algal biomass productivity of 47 gDW/(m2. d) in the blue AG-PBRs was found to be higher than those of other algal attached-growth systems. Although, the red and blue AG-PBR systems could effectively treat the septic tank effluent to meet the national and international discharge standards, based on treatment efficiencies and biomass productivity, the blue AG-PBR is recommended for treatment of septic tank effluent.

Removal and monitoring acetaminophen-contaminated hospital wastewater by vertical flow constructed wetland and peroxidase enzymes.

Hospital wastewater contains acetaminophen (ACT) and nutrient, which need adequate removal and monitoring to prevent impact to environment and community. This study developed a pilot scale vertical flow constructed wetland (CW) to (1) remove high-dose ACT and pollutants in hospital wastewater and (2) identify the correlation of peroxidase enzyme extruded by Scirpus validus and pollutants removal efficiency. By that correlation, a low-cost method to monitor pollutants removal was drawn. Plants, such as Scirpus validus, generated peroxidase enzymes to alleviate pollutants' stress. Results showed that the CW removed 3.5 to 6 logs of initial concentration 10 mg ACT/L to a recommended level for drinking water. The CW eliminated COD, TKN and TP efficiently, meeting the wastewater discharged standards of Thailand and Vietnam. By various multivariable regression models, concentrations of ACT in CW effluent and enzymes in S. validus exhibited a significant correlation (p < 0.01, R2 = 68.3%). These findings suggested that (i) vertical flow CW could remove high-dose ACT and nutrient and (ii) peroxidase enzymes generated in S. validus, such as soluble and covalent ones, could track ACT removal efficiency. This would help to reduce facilities and analytical cost of micro-pollutants.

Determination and health risk assessment of enrofloxacin, flumequine and sulfamethoxazole in imported Pangasius catfish products in Thailand.

The goals of this study were to determine the levels of three antibiotics - enrofloxacin, flumequine and sulfamethoxazole - in Pangasius catfish products imported into Thailand and to assess the health risks from consumption. To extract these antibiotic residues, acetonitrile, methanol and a small amount of formic acid were used as solvents. Determination of the antibiotics after extraction steps was carried out by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) technique. The results showed that 14 and 3 samples of Pangasius catfish products were contaminated with enrofloxacin and sulfamethoxazole, respectively. No flumequine residue was found. While the concentration levels of these antibiotics in most contaminated samples were lower than the European Union (EU) standard, one sample was found to contain sulfamethoxazole at 245.91 µg kg-1, which was higher than the EU standard (100 µg kg-1), indicating the likelihood that some contaminated freshwater fish products are widely distributed in Thai markets. Notably, the concentration levels of enrofloxacin in samples of Pangasius catfish with skin were higher than in non-skin products, suggesting that products with skin might retain more antibiotic residues than non-skin products. Although the hazard quotient showed that consuming imported Pangasius catfish products, based on the current consumption rate, will not adversely affect consumer health, antibiotic residues in Pangasius catfish products imported into Thailand should be continually monitored.

Faecal sludge treatment and utilization by hydrothermal carbonization.

Hydrothermal carbonization (HTC) is a thermal conversion process that can be applied to convert faecal sludge into carbonaceous solids, called hydrochar. In this study, the technical feasibility of hydrochar production by HTC of faecal sludge was investigated. Experimental results showed energy contents of the produced hydrochar to be about 19-20 MJ/kg, comparable to natural coals and therefore usable as a solid fuel. The produced hydrochar contained a carbon content of approximately 40%wt, which could be processed further to make it suitable as an anode in batteries. The produced hydrochar also had adsorption characteristics for removing heavy metals and micropollutants in wastewater. Liquid by-products obtained from the HTC process were found to contain high concentrations of organic matter, while the amount of gas produced was 10 L-gas/kg-FS with CO2 is the main component. The bio-methane potential tests of this liquid product suggested the methane production of about 2.0 L-CH4 per kg-faecal sludge could be obtained.

Performance and kinetics of algal-bacterial photobioreactor (AB-PBR) treating septic tank effluent.

Septic tank effluent contains high organic and nutrient contents. This study aimed to evaluate treatment performance of an algal-bacterial photobioreactor (AB-PBR) treating the septic tank effluent. The experimental unit employed a transparent plastic medium made from recycled drinking water bottles for attached-growth biofilm. Red LED lamp (light intensity ∼100 µmol/m2/s) was applied as an energy source for the growth of algal-bacterial biofilm in the AB-PBR. The experimental results showed that AB-PBR operated at the hydraulic retention time (HRT) of 3 days gave the highest chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) removal efficiencies of 64, 45 and 35%, respectively, by which the effluent COD concentrations could meet the effluent standards of Thailand, but the effluent TN and TP concentrations needed to be further removed. The Stover-Kincannon model was applied to determine the kinetic values of COD and TN removals with R2 values greater than 0.8. Microbiological examinations indicated Chlorella sp. is the predominant algal species growing in the AB-PBR, while the amplicon sequencing information analytical results revealed the bacterial phylum of Proteobacteria to be the predominant bacterial group.

Monitoring and determination of sulfonamide antibiotics (sulfamethoxydiazine, sulfamethazine, sulfamethoxazole and sulfadiazine) in imported Pangasius catfish products in Thailand using liquid chromatography coupled with tandem mass spectrometry.

This research aimed to monitor the concentrations of sulfamethoxydiazine (SMD), sulfamethazine (SMT), sulfamethoxazole (SMX) and sulfadiazine (SDZ) in imported Pangasius catfish products in Thailand. The residues of the four sulfonamides (SAs) were analyzed by extraction process and liquid chromatography coupled with tandem mass spectrometry. The highest concentrations found were 10.97ng/g for SMD, 6.23ng/g for SMT, 11.13ng/g for SDZ and 245.91ng/g for SMX, which was higher than the European Union (EU) standard (100ng/g). Moreover, all samples contaminated with SMX also contained SMT, indicating that more than one antibiotic was used for production in the country of origin. Because Thai standards for antibiotics in food have not been completely set, all contaminated discovered would not be considered to be an illegal food, in which antibiotic residues may affect human health in the long term. Therefore, antibiotic residues in Pangasius catfish products should be continually regulated and monitored.

Evaluation of removal efficiency of human antibiotics in wastewater treatment plants in Bangkok, Thailand.

This study aimed to investigate the antibiotic concentration at each stage of treatment and to evaluate the removal efficiency of antibiotics in different types of secondary and advanced treatment, as well as the effects of the location of their discharge points on the occurrence of antibiotics in surface water. Eight target antibiotics and four hospital wastewater treatment plants in Bangkok with different conventional and advanced treatment options were investigated. Antibiotics were extracted by solid phase extraction and analysed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The antibiotic with the highest concentration at influent was cefazolin at 13,166 ng/L, while the antibiotic with the highest concentration at effluent was sulfamethoxazole at 1,499 ng/L. The removal efficiency of antibiotics from lowest to highest was sulfamethoxazole, piperacillin, clarithromycin, metronidazole, dicloxacillin, ciprofloxacin, cefazolin, and cefalexin. The adopted conventional treatment systems could not completely remove all antibiotics from wastewater. However, using advanced treatments or disinfection units such as chlorination and UV could increase the antibiotic removal efficiency. Chlorination was more effective than UV, ciprofloxacin and sulfamethoxazole concentration fluctuated during the treatment process, and sulfamethoxazole was the most difficult to remove. Both these antibiotics should be studied further regarding their contamination in sludge and suitable treatment options for their removal.

Enhancement of Cr(VI) Ion Removal Using Nanochitosan Coated on Bituminous Activated Carbon.

Bituminous activated carbon (AC) has been widely used as a sorbent for adsorption of non-polar species, but its performance for removal of ionic species such as heavy metals has not been as efficient. In this study, AC was modified with chitosan nanoparticles (CN) using facile methods of dip coating and wet impregnation. The CN-coated AC demonstrated an increase in Cr(VI) removal efficiency in both kinetics and adsorption capacity. The adsorption capacity of the CN-coated AC (mg/g) was more than twice that of the uncoated AC (36.36 mg/g), or pure chitosan (32.57 mg/g). The sizes of the synthesized CN (160-2,000 nm) can be controlled by varying the concentration of the chitosan/reagents used. The adsorption isotherms are better described using the Freundlich rather than the Langmuir model and are in agreement with the heterogeneity of the surfaces. Adsorption kinetics followed that of the pseudo-second-order kinetics, suggesting chemisorption as a rate limiting step.