ABSTRACT
Mango leaf tea has been traditionally used by different cultures to reduce inflammation in the body. There is evidence that chronic inflammation increases the risk of cancer. This study investigates the antitumoural effects of pressurized mango leaf extracts on minimally (MCF7) and highly invasive (MDA-MB-231) breast cancer cells as well as on non-tumourigenic cells (MCF10). Extracts showed protective properties against oxidation and cytotoxic effects against breast cancer cell lines, causing minor damage to non-carcinogenic cells. Nonetheless, some selective activity, depending on hormone receptor status, was observed. This was possibly related to the presence of minor compounds. Extracts with high levels of gallotannins showed cytotoxic action against MCF7 cells, while those which had methyl gallate and homomangiferin as common components were more effective against MDA-MB-231 cells. Therefore, the cytotoxic effect of mango leaf extracts might be attributed to the synergistic effect of different polyphenols and not just to mangiferin on its own as the predominant compound in mango leaves.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Breast Neoplasms/drug therapy , Mangifera/chemistry , Plant Extracts/pharmacology , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Apoptosis/drug effects , Breast Neoplasms/pathology , Breast Neoplasms/physiopathology , Cell Line, Tumor , Female , Humans , Plant Extracts/chemistry , Plant Extracts/isolation & purification , Plant Leaves/chemistryABSTRACT
Glandularia perakii is a perennial species with beautiful violet flowers that grows in the stony soil of Mendocine pedemont. A plentiful and prolonged flowering confers it an important ornamental potential. In this paper, a method of propagation of G. perakii from nodal segments is reported. Proliferating microshoot cultures were obtained by placing nodal segment on Murashige and Skoog medium (MS) supplemented with 20 g.L-1 of sucrose without growth regulators. In this medium multiplication rate after 20 days was 7.9. Rooted plants were acclimatized successfully. (AU)
Subject(s)
RESEARCH SUPPORT, NON-U.S. GOVT , Botany/methods , Plant Physiological Phenomena , Verbenaceae/growth & development , Acclimatization , Indoleacetic Acids/metabolism , Indoleacetic Acids/pharmacology , Cells, Cultured , Culture Media , Plant Shoots/drug effects , Plant Shoots/growth & development , Plant Shoots/physiology , Reproduction , Verbenaceae/drug effects , Verbenaceae/physiologyABSTRACT
Glandularia perakii is a perennial species with beautiful violet flowers that grows in the stony soil of Mendocine pedemont. A plentiful and prolonged flowering confers it an important ornamental potential. In this paper, a method of propagation of G. perakii from nodal segments is reported. Proliferating microshoot cultures were obtained by placing nodal segment on Murashige and Skoog medium (MS) supplemented with 20 g.L-1 of sucrose without growth regulators. In this medium multiplication rate after 20 days was 7.9. Rooted plants were acclimatized successfully.
Subject(s)
Botany/methods , Plant Physiological Phenomena , Verbenaceae/growth & development , Acclimatization , Plant Shoots/drug effects , Plant Shoots/growth & development , Plant Shoots/physiology , Cells, Cultured , Culture Media , Indoleacetic Acids , Reproduction , Verbenaceae/drug effects , Verbenaceae/physiologyABSTRACT
Thromboxane B2 (TxB2) the stable metabolite of thromboxane A2 may be released as a response to ischemia. With the aim of investigating its role as an early diagnostic test in mesenteric thrombosis, immunoreactive TxB2 was measured in urine aliquotes in six sham operated dogs, nine dogs subjected to superior mesenteric artery ligation, and twelve dogs with superior mesenteric vein ligation. One hour urine volumes were collected before surgery and during the eight hours after the experimental procedures, and urinary osmolarities were also determined in each sample. Basal TxB2 levels were comparable in all groups. Although all groups showed a significant and rapid (one hour) increase in TxB2 as a response to surgery, in the controls it returned to normal after six hours, whereas in the rest a continuously increased production persisted throughout the study period. There was no difference in t-test comparisons depending on the sort of thrombosis. In spite of the urinary dilution induced during the study, a persistent increase in TxB2 excretion was found. We conclude that urinary TxB2 levels could prove useful in the early diagnosis of mesenteric ischemia.
