ABSTRACT
The dibenzylbutyrolactone lignan (-)-hinokinin (HK) was obtained by partial synthesis from (-)-cubebin, isolated from the dry seeds of the pepper, Piper cubeba. In view of the trypanocidal activity of HK and its potential as a lead compound for drug development, evaluation of its possible genotoxic activity is required. We have tested HK for possible genotoxicity and evaluated the compound's effect on the activity of the clastogens doxorubicin (DXR) and methyl methanesulfonate (MMS) in the micronucleus (MN) assay with Chinese hamster lung fibroblast V79 cells. HK alone did not induce MN, at concentrations up to 128microM. In combined treatments, HK reduced the frequency of MN induced by MMS. With respect to DXR, HK exerted a protective effect at lower concentrations, but at higher concentrations it potentiated DXR clastogenicity.
Subject(s)
4-Butyrolactone/analogs & derivatives , Dioxoles/toxicity , Doxorubicin/toxicity , Lignans/toxicity , Methyl Methanesulfonate/toxicity , Mutagens/toxicity , 4-Butyrolactone/pharmacology , 4-Butyrolactone/toxicity , Animals , Benzodioxoles , Cell Line , Cricetinae , Cricetulus , Dioxoles/pharmacology , Dose-Response Relationship, Drug , Drug Interactions , Fibroblasts/drug effects , Lignans/pharmacology , Lung/drug effects , Micronucleus TestsABSTRACT
¿Qué es la Odontología Forense? ¿Cuál es su utilidad en nuestro país? ¿Cuáles son las técnicas odontológicas forenses más utilizadas en El Salvador? Estas son algunas de las preguntas fundamentales que este trabajo intenta responde, a través de una investigación realizada en el Instituto de Medicina Legal "Dr. Roberto Masferrer", y con el aporte de los odontólogos forenses que trabajan en las diferentes dependencias de ese instituto, a quienes se les solicitó su colaboración para contestar una serie de preguntas referidas al tema en estudio Con los resultados de esas entrevistas, se obtuvo importante información sobre las técnicas odontológicas forenses utilizadas. Dichos resultados han sido debidamente analizados, cuantitativa y cualitativamente, para lo cual se incluyen cuadros estadísticos Toda la información recogida, como se verá en el desarrollo de esta investigación, resultó muy novedosa e interesante. V El trabajo además incluye un apartado de objetivos, limitantes, alcances y otros, así como también un extenso y completo marco teórico y una explicación detallada de la metodología que se utilizó a lo largo de esta investigación. Se espera que, con este trabajo, las personas que desean conocer aspectos relevantes sobre las técnicas odontológicas forenses, obtengan conocimientos fundamentales y de interés general en el área de la Odontología Forense.
What is Forensic Dentistry? What is its usefulness in our country? What are the most used forensic dental techniques in El Salvador? These are some of the fundamental questions that this work tries to answer, through an investigation carried out in the Institute of Legal Medicine "Dr. Roberto Masferrer", and with the contribution of forensic dentists who work in the different dependencies of that institute, Their collaboration was requested to answer a series of questions related to the subject under study. With the results of these interviews, important information was obtained on the forensic dental techniques used. These results have been duly analyzed, quantitatively and qualitatively, for which statistical tables are included. All the information collected, as will be seen in the development of this research, was very novel and interesting. V The work also includes a section on objectives, limitations, scope and others, as well as an extensive and complete theoretical framework and a detailed explanation of the methodology used throughout this research. It is expected that, with this work, people who want to know relevant aspects of forensic dental techniques, obtain fundamental knowledge of general interest in the area of ââForensic Dentistry.
