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1.
Oncogene ; 35(35): 4663-74, 2016 09 01.
Article in English | MEDLINE | ID: mdl-26876207

ABSTRACT

Targeted therapies using small-molecule inhibitors (SMIs) are commonly used in metastatic renal cell cancer (mRCC) patients; patients often develop drug resistance and eventually succumb to disease. Currently, understanding of mechanisms leading to SMIs resistance and any identifiable predictive marker(s) are still lacking. We discovered that DAB2IP, a novel Ras-GTPase-activating protein, was frequently epigenetically silenced in RCC, and DAB2IP loss was correlated with the overall survival of RCC patients. Loss of DAB2IP in RCC cells enhances their sensitivities to growth factor stimulation and resistances to SMI (such as mammalian target of rapamycin (mTOR) inhibitors). Mechanistically, loss of DAB2IP results in the activation of extracellular signal-regulated kinase/RSK1 and phosphoinositide-3 kinase/mTOR pathway, which synergizes the induction of hypoxia-inducible factor (HIF)-2α expression. Consequently, elevated HIF-2α suppresses p21/WAF1 expression that is associated with resistance to mTOR inhibitors. Thus combinatorial targeting both pathways resulted in a synergistic tumor inhibition. DAB2IP appears to be a new prognostic/predictive marker for mRCC patients, and its function provides a new insight into the molecular mechanisms of drug resistance to mTOR inhibitors, which also can be used to develop new strategies to overcome drug-resistant mRCC.


Subject(s)
Carcinoma, Renal Cell/genetics , Cell Proliferation/genetics , Drug Resistance, Neoplasm/genetics , TOR Serine-Threonine Kinases/genetics , ras GTPase-Activating Proteins/genetics , Adult , Aged , Animals , Carcinoma, Renal Cell/drug therapy , Carcinoma, Renal Cell/pathology , Cell Line, Tumor , Cell Proliferation/drug effects , Female , Gene Expression Regulation, Neoplastic/drug effects , Humans , Male , Middle Aged , Molecular Targeted Therapy , Protein Kinase Inhibitors/administration & dosage , Signal Transduction/genetics , Sirolimus/administration & dosage , TOR Serine-Threonine Kinases/antagonists & inhibitors , ras GTPase-Activating Proteins/biosynthesis
2.
Cell Death Dis ; 6: e1955, 2015 Oct 29.
Article in English | MEDLINE | ID: mdl-26512963

ABSTRACT

Loss of DAB2IP, a novel tumor suppressor gene, is associated with the high risk of aggressive prostate cancer (PCa). Previously, we reported that DAB2IP modulated androgen receptor activation in the development of castration-resistant PCa; however, its direct action on the failure of androgen deprivation therapy (ADT) remains largely unknown. In this study, we showed that DAB2IP knockdown could significantly enhance in vitro growth and colony formation of PCa cells following ADT as well as tumorigenicity in pre-castrated nude mice. In addition, DAB2IP loss stabilized mitochondrial transmembrane potential, prevented release of cytochrome c, Omi/HtrA2 and Smac from the mitochondria to the cytoplasm and inhibited intrinsic apoptosis induced by ADT. Mechanistically, DAB2IP could interact with the signal transducer and activator of transcription 3 (STAT3) via its unique PR domain and suppress STAT3 phosphorylation and transactivation, leading to the inhibition of survivin expression in PCa cells. Moreover, the luminal epithelia in DAB2IP(-/-) mice with more activated STAT3 and survivin expression were resistant to castration-induced apoptosis. Consistently, DAB2IP expression inversely correlated with STAT3 phosphorylation and survivin expression in PCa patients. Together, our data indicate that DAB2IP loss reprograms intracellular signal transduction and anti-apoptotic gene expression, which potentiates PCa cell survival from ADT-induced cell death.


Subject(s)
Prostatic Neoplasms, Castration-Resistant/genetics , ras GTPase-Activating Proteins/genetics , Animals , Apoptosis , Castration , Cytochromes c/metabolism , Gene Deletion , HEK293 Cells , Humans , Inhibitor of Apoptosis Proteins/metabolism , Male , Membrane Potential, Mitochondrial/genetics , Mice , Mice, Nude , Phosphorylation , Prostatic Neoplasms, Castration-Resistant/pathology , Repressor Proteins/metabolism , STAT3 Transcription Factor/metabolism , STAT3 Transcription Factor/physiology , Signal Transduction/genetics , Survivin , ras GTPase-Activating Proteins/metabolism , ras GTPase-Activating Proteins/physiology
3.
Cancer Res ; 61(17): 6592-600, 2001 Sep 01.
Article in English | MEDLINE | ID: mdl-11522659

ABSTRACT

The coxsackie and adenovirus receptor (CAR) is identified as a high-affinity receptor for adenovirus type 5. We observed that invasive bladder cancer specimens had significantly reduced CAR mRNA levels compared with superficial bladder cancer specimens, which suggests that CAR may play a role in the progression of bladder cancer. Elevated CAR expression in the T24 cell line (CAR-negative cells) increased its sensitivity to adenovirus infection and significantly inhibited its in vitro growth, accompanied by p21 and hypophosphorylated retinoblastoma accumulation. Conversely, decreased CAR levels in both RT4 and 253J cell lines (CAR-positive cells) promoted their in vitro growth. To unveil the mechanism of action of CAR, we showed that the extracellular domain of CAR facilitated intercellular adhesion. Furthermore, interrupting intercellular adhesion of CAR by a specific antibody alleviates the growth-inhibitory effect of CAR. We also demonstrated that both the transmembrane and intracellular domains of CAR were critical for its growth-inhibitory activity. These data indicate that the cell-cell contact initiated by membrane-bound CAR can elicit a negative signal cascade to modulate cell cycle regulators inside the nucleus of bladder cancer cells. Therefore, the presence of CAR cannot only facilitate viral uptake of adenovirus but also inhibit cell growth. These results can be integrated to formulate a new strategy for bladder cancer therapy.


Subject(s)
Receptors, Virus/physiology , Urinary Bladder Neoplasms/pathology , Adenoviridae/physiology , Aged , Aged, 80 and over , Cell Adhesion/physiology , Cell Aggregation/physiology , Cell Division/physiology , Coxsackie and Adenovirus Receptor-Like Membrane Protein , Cyclin-Dependent Kinase Inhibitor p21 , Cyclins/biosynthesis , Female , Growth Inhibitors/biosynthesis , Growth Inhibitors/genetics , Growth Inhibitors/physiology , Humans , Male , Middle Aged , Nuclear Proteins/biosynthesis , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Virus/biosynthesis , Receptors, Virus/genetics , Structure-Activity Relationship , Transfection , Urinary Bladder Neoplasms/genetics , Urinary Bladder Neoplasms/metabolism , Urinary Bladder Neoplasms/virology
4.
Cancer Res ; 60(18): 5031-6, 2000 Sep 15.
Article in English | MEDLINE | ID: mdl-11016624

ABSTRACT

In a recent paper, we reported a significant difference in coxsackie and adenovirus receptor (CAR) from several human bladder cancer cell lines that correlated with their sensitivities to adenoviral infection (Y. Li, R-C. Pong, J. M. Bergelson, M. C. Hall, A. I. Sagalowsky, C-P. Tseng, Z. Wang, and J. T. Hsieh, Cancer Res., 59: 325-330, 1999). In human prostate cancer, CAR protein is down-regulated in the highly tumorigenic PC3 cell line, which suggests that, in addition to its function as a viral receptor, CAR may have a pathophysiological role in prostate cancer progression. In this paper, we document that CAR does not merely enhance the viral sensitivity of prostate cancer cells but also acts as a tumor inhibitor for androgen-independent prostate cancer cells. Our data indicate that CAR is a potential therapeutic agent for increasing the efficacy of prostate cancer therapy.


Subject(s)
Genetic Therapy , Prostatic Neoplasms/therapy , Receptors, Virus/physiology , Adenoviridae/genetics , Animals , Cell Division/physiology , Coxsackie and Adenovirus Receptor-Like Membrane Protein , Genetic Vectors , Humans , Male , Mice , Mice, Nude , Neoplasm Transplantation , Prostatic Neoplasms/metabolism , Prostatic Neoplasms/pathology , Receptors, Virus/biosynthesis , Receptors, Virus/genetics , Transfection , Tumor Cells, Cultured
5.
J Urol ; 163(3): 1033-8, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10688044

ABSTRACT

PURPOSE: To evaluate whether p21 (WAF-1/CIP1) should be considered a potential candidate for human bladder cancer gene therapy, we determined: (1) the basal level of p21 expression in bladder cancer cell lines, (2) the response of bladder cancer cells to increased p21 expression following p21 adenovirus infection, and (3) the mechanism of growth inhibition produced by p21 overexpression. MATERIALS AND METHODS: Five established human bladder cancer cell lines and one primary culture derived from an invasive transitional cell carcinoma were used in this study. To examine the effect of p21 protein on the growth of human bladder cancer cells, a recombinant adenovirus vector system containing p21 cDNA, under the control of cytomegalovirus promoter, was constructed. A control virus containing p21 in an antisense orientation was used to eliminate potential artifacts caused by viral toxicity. RESULTS: Human bladder cancer cell lines exhibit variable endogenous p21 levels which correlate with the in vitro growth status. Significant, but highly variable increases in the steady-state level of p21 were detected in p21 adenovirus infected cells. Human bladder cancer cell lines responded heterogeneously to p21 adenovirus infection. Growth of the WH cell line was substantially inhibited in a dose and time-course dependent fashion. The mechanism of p21 growth inhibition was found to be due to G0/G1 arrest and not the induction of apoptosis. In contrast, p21 adenovirus failed to inhibit the growth of T24 bladder cancer cells because T24 cells were resistant to viral infection. The 253J bladder cancer cells exhibited marked sensitivity to adenovirus; substantial growth inhibition was seen with both sense and antisense p21 very early in the time course of infection. CONCLUSIONS: We found significant variation in the basal level of p21 protein expression in several human bladder cancer cell lines. Increased p21 expression as a result of adenoviral infection may be a potent growth suppressor in some human bladder cancer because it elicits cell cycle arrest in G0/G1 stage, but not the induction of apoptosis. Bladder cancer cells exhibit a wide spectrum of sensitivity to adenoviral infection that may be caused by the presence of viral receptor heterogeneity. This wide spectrum of sensitivity has significant basic scientific and clinical implications and warrants further study.


Subject(s)
Adenoviridae/genetics , Carcinoma, Transitional Cell/metabolism , Cyclins/biosynthesis , Urinary Bladder Neoplasms/metabolism , Carcinoma, Transitional Cell/genetics , Carcinoma, Transitional Cell/pathology , Carcinoma, Transitional Cell/therapy , Cell Division , Cyclin-Dependent Kinase Inhibitor p21 , Cyclins/therapeutic use , G1 Phase , Gene Expression Regulation, Neoplastic , Gene Expression Regulation, Viral , Genetic Therapy , Humans , Resting Phase, Cell Cycle , Tumor Cells, Cultured , Urinary Bladder Neoplasms/genetics , Urinary Bladder Neoplasms/pathology , Urinary Bladder Neoplasms/therapy
6.
J Biol Chem ; 274(45): 31981-6, 1999 Nov 05.
Article in English | MEDLINE | ID: mdl-10542228

ABSTRACT

DOC-2/DAB2, a novel phosphoprotein with signal-transducing capability, inhibits human prostatic cancer cells (Tseng, C.-P., Ely, B. D., Li, Y., Pong, R.-C., and Hsieh, J.-T. (1998) Endocrinology 139, 3542-3553). However, its mechanism of action is not understood completely. This study delineates the functional significance of DOC-2/DAB2 protein phosphorylation and demonstrates that in vivo activation of protein kinase C (PKC) by 12-O-tetradecanoylphorbol-13-acetate (TPA) induces DOC-2/DAB2 phosphorylation, including a serine residue at position 24. Mutation of Ser(24) to Ala reduced DOC-2/DAB2 phosphorylation by PKC. Using a synthetic Ser(24) peptide (APS(24)KKEKKKGSEKTD) or recombinant DOC-2/DAB2 as substrates, PKCbetaII, PKCgamma, and PKCdelta (but not casein kinase II) directly phosphorylated Ser(24) in vitro. This indicates that DOC-2/DAB2 is a PKC-specific substrate. Since expression of wild-type DOC-2/DAB2, but not the S24A mutant, inhibited TPA-induced AP-1 activity in prostatic epithelial cells, phosphorylation of Ser(24) appears to play a critical role in modulating TPA-induced AP-1 activity. Taken together, these data suggest that PKC-regulated phosphorylation of DOC-2/DAB2 protein may help its growth inhibitory function.


Subject(s)
Adaptor Proteins, Vesicular Transport , Genes, Tumor Suppressor , Prostatic Neoplasms/metabolism , Proteins/metabolism , Transcription Factor AP-1/antagonists & inhibitors , Adaptor Proteins, Signal Transducing , Amino Acid Sequence , Animals , Apoptosis Regulatory Proteins , COS Cells , Humans , Male , Molecular Sequence Data , Phosphorylation , Protein Kinase C/metabolism , Serine/metabolism , Tetradecanoylphorbol Acetate/pharmacology , Tumor Suppressor Proteins
7.
Prostate ; 41(1): 31-8, 1999 Sep 15.
Article in English | MEDLINE | ID: mdl-10440873

ABSTRACT

BACKGROUND: Recently, we demonstrated that expression of C-CAM1, an immunoglobulin (Ig)-like cell adhesion molecule (CAM), was diminished in both prostate intraepithelial neoplasia and cancer lesions, indicating that loss of C-CAM1 expression may be involved in the early events of prostate carcinogenesis. Also, increased C-CAM1 expression can effectively inhibit the growth of prostate cancer. Structurally, C-CAM1 represents a unique CAM with a potential signal transducing capability. In this study, we further analyzed the functional domain of C-CAM1 for controlling its tumor suppression function. METHODS: Recombinant adenoviruses expressing a series of C-CAM1 mutants were generated, such as AdCAMF488 (mutated C-CAM1 containing Tyr-488 --> Phe-488), AdCAMH458 (intracellular domain deletion mutant containing 458 amino acids), AdCAMG454 (intracellular domain deletion mutant containing 454 amino acids), and AdCAMDeltaD1(C-CAM1 mutant containing first Ig domain deletion). After in vitro characterization of each virus, human prostate cancer cells infected with these viruses were subcutaneously injected into athymic mouse. Both tumor incidence and volume were measured for determining the tumor suppression function for each mutant. RESULTS: In vivo tumorigenic assay indicated that AdCAMDeltaD1 without cell adhesion function still retained its tumor suppression activity. In contrast, both AdCAMH458 and AdCAMG454 decreased or lost their tumor suppression activity. CONCLUSIONS: Our data indicate that the intracellular domain of the C-CAM1 molecule is critical for inhibiting the growth of prostate cancer, suggesting that C-CAM1 interactive protein(s) may dictate prostate carcinogenesis.


Subject(s)
Adenosine Triphosphatases/chemistry , Adenosine Triphosphatases/metabolism , Cell Adhesion Molecules/chemistry , Cell Adhesion Molecules/metabolism , Genes, Tumor Suppressor/physiology , Prostatic Neoplasms/metabolism , Adenosine Triphosphatases/genetics , Adenoviridae/genetics , Adenoviridae/growth & development , Amino Acid Substitution , Animals , Antigens, CD , Carcinoembryonic Antigen , Cell Adhesion , Cell Adhesion Molecules/genetics , Gene Expression , Genes, Tumor Suppressor/genetics , Genetic Vectors/genetics , Glycoproteins , Humans , Male , Mice , Mice, Nude , Neoplasm Transplantation , Prostatic Neoplasms/pathology , Protein Conformation , Sequence Deletion , Structure-Activity Relationship , Tumor Cells, Cultured , Viral Plaque Assay
8.
Oncogene ; 18(17): 2747-54, 1999 Apr 29.
Article in English | MEDLINE | ID: mdl-10348349

ABSTRACT

The Deleted in Colorectal Cancer gene (DCC) encodes a cell surface receptor that belongs to the Ig superfamily. Inactivation of the DCC gene has been implicated in human tumor progression. However, little is known about the biological function of the DCC protein. In the present study, we demonstrated that expression of DCC activated caspase-3 and programmed cell death, or induced G2/M cell cycle arrest in tumor cells. In some cell lines, apoptosis was evident within 24 h of DCC expression. Timing of the appearance of apoptotic cells coincided with that of the cleavage of poly (ADP-ribose) polymerase, a substrate of caspase-3. Expression of the apoptosis inhibitory gene Bcl-2 was not able to abrogate the DCC-induced apoptosis. In the G2/M cycle arrest cells, cdk1 activity was inhibited. Our results suggest that the DCC protein may transduce signals resulting in activation of caspases or inhibition of Cdk1. These data provide a possible mechanism by which DCC suppresses tumorigenesis.


Subject(s)
Apoptosis/physiology , Chromosomes, Human, Pair 18 , Colorectal Neoplasms/genetics , G2 Phase/physiology , Gene Deletion , Mitosis/physiology , Humans , Loss of Heterozygosity , Tumor Cells, Cultured
9.
J Virol ; 73(6): 4983-90, 1999 Jun.
Article in English | MEDLINE | ID: mdl-10233960

ABSTRACT

p21((WAF1/SDII/CIP1)) (p21) arrests cell growth by inhibiting cyclin-depend kinases. To explore the potential of using p21 for the gene therapy of cervical cancer, we infected human papillomavirus (HPV)-positive cervical cancer cells (HeLa, SiHa, and Z172) and HPV-negative cervical cancer cells (C33A) with recombinant adenovirus encoding p21 cDNA. The results revealed that effective inhibition of cell growth could be achieved by sense p21 adenovirus but not antisense p21 adenovirus infection and occurred through apoptosis as measured by DNA fragmentation and chromatin condensation. Apoptosis was also observed in xenografts of human cervical cancer cells infected with sense p21 adenovirus, as confirmed by in situ terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL). The apoptosis was not prevented by overexpression of the bcl-2 transgene. To sum up, the apoptotic effect suggests that p21 should be a tumoricidal agent instead of a tumoristatic agent in preventing cervical cancers. In addition, our report substantiates the combination of the high efficiency of adenovirus vector-mediated gene delivery and the apoptotic effect of p21.


Subject(s)
Adenoviridae/genetics , Apoptosis , Cyclins/genetics , Genetic Therapy , Uterine Cervical Neoplasms/therapy , Animals , Cell Division , Cyclin-Dependent Kinase Inhibitor p21 , Cyclins/physiology , Female , Gene Transfer Techniques , Humans , In Situ Nick-End Labeling , Mice , Mice, Nude , Neoplasm Transplantation , Proto-Oncogene Proteins c-bcl-2/physiology , Transplantation, Heterologous , Tumor Cells, Cultured , Uterine Cervical Neoplasms/pathology
10.
Cancer Res ; 59(2): 325-30, 1999 Jan 15.
Article in English | MEDLINE | ID: mdl-9927041

ABSTRACT

There is great interest in the development of gene therapeutic strategies for the treatment of benign and malignant diseases. Recombinant adenovirus has a wide spectrum of tissue specificity and is an efficient vector delivery system. Successful gene delivery, however, requires viral entry into the target cells via specific receptor-mediated uptake. Recently, a cDNA clone (the coxsackie and adenovirus receptor [CAR]) encoding a 46-kDa protein was identified as the receptor for group C adenovirus (e.g., adenovirus type 2 and 5). Currently, little is known regarding the expression of adenoviral receptor in normal tissue and cancer. In this paper, we have documented a significant difference in viral receptor levels that may be due to transcriptional regulation of the CAR gene in several human bladder cancer cell lines. The differences in viral receptor levels in these cells correlated with their sensitivity to viral infection. Transfection of receptor-negative cell line with CAR cDNA led to increased virus binding and increased susceptibility to adenovirus-mediated gene delivery. Our results demonstrate that the expression of adenoviral receptor is variable among human bladder cancer cells. This variability may have a significant impact on the outcome of adenovirus-based gene therapy.


Subject(s)
Adenoviridae/genetics , Genetic Therapy , Receptors, Virus/physiology , Urinary Bladder Neoplasms/therapy , Humans , RNA, Messenger/analysis , Receptors, Virus/genetics , Transfection , Tumor Cells, Cultured , Urinary Bladder/virology
11.
Clin Cancer Res ; 5(1): 197-202, 1999 Jan.
Article in English | MEDLINE | ID: mdl-9918219

ABSTRACT

Previous studies have shown that the negative cell cycle regulator WAF1/Cip1 is often overexpressed in human gliomas and that WAF1/Cip1 overexpression renders glioma cells resistant to chemotherapy agents. In this study, we investigated whether down-regulation of WAF1/Cip1 would sensitize gliomas to chemotherapy. An adenoviral vector expressing antisense WAF1/Cip1 was constructed and used to infect D54 glioma cells, which express a high level of endogenous WAF1/Cip1. After D54 cells were infected with antisense WAF1/Cip1 adenovirus, Western blotting revealed a significant decrease in the WAF1/Cip1 protein level. Down-regulation of WAF1/Cip1 alone resulted in the cells rounding up and detaching from plates. Electron microscopy revealed some nuclear fragmentation in antisense WAF1/Cip1-infected cells, indicating the initiation of apoptosis. The antisense WAF1/Cip1-infected cells were then treated with the chemotherapeutic agents 1,3-bis(2-chloroethyl)-1-nitrosourea and cisplatin. Other cells were infected with sense WAF1/Cip1 adenovirus or control virus and served as controls. Trypan blue exclusion assay revealed significant cell death in antisense WAF1/Cip1-infected cells. In situ end-labeling assay by flow cytometry revealed that many cells died of apoptosis. Our results show that the attenuation of WAF1/Cip1 expression initiated glioma cell death and sensitized glioma cells to apoptosis induced by 1,3-bis(2-chloroethyl)-1-nitrosourea and cisplatin. Thus, blocking WAF1/Cip1 production may serve as a useful chemosensitization regimen for treating glioma.


Subject(s)
Antineoplastic Agents, Alkylating/pharmacology , Antineoplastic Agents/pharmacology , Apoptosis/genetics , Carmustine/pharmacology , Cisplatin/pharmacology , Cyclins/biosynthesis , Glioblastoma/drug therapy , Oligodeoxyribonucleotides, Antisense/pharmacology , Adenoviridae/genetics , Apoptosis/drug effects , Cyclin-Dependent Kinase Inhibitor p21 , Cyclins/genetics , DNA, Complementary/genetics , Down-Regulation/drug effects , Down-Regulation/genetics , Genetic Vectors , Glioblastoma/metabolism , Glioblastoma/pathology , Humans , Tumor Cells, Cultured
12.
Endocrinology ; 139(8): 3542-53, 1998 Aug.
Article in English | MEDLINE | ID: mdl-9681506

ABSTRACT

Androgen is a mitogen as well as a morphogen for prostatic epithelium. However, the detailed mechanisms of these distinct androgenic actions have not yet been delineated. Therefore, we employed differential display PCR to unveil any potential genes that may be involved in these processes. In this study, we report the isolation and characterization of two alternative splicing forms (p82 and p59) of C9 complementary DNA, the rat homolog of the human deletion of ovarian carcinoma 2 (DOC-2) gene and mouse p96 phosphoprotein, from rat ventral prostate (VP). We found that C9 was up-regulated in rat VP after castration, suggesting that C9 may be regulated by androgen receptor directly or indirectly during prostate degeneration. A similar regulatory pattern was also observed in both the seminal vesicle and dorsolateral prostate, but not in the coagulating gland or other androgen-independent organs. Immunohistochemical analysis of rat VP demonstrated that C9 is detected in the basal epithelia and surrounding stromal cells after prolonged castration. Ribonuclease protection assay and Western blot analysis revealed that p59 is the predominant C9 isoform in rat VP. To unveil the function of C9 in cell growth, we transfected p59 complementary DNA into the C4-2 cells, a derivative of the LNCaP prostatic carcinoma cell line. The p59 stable transfectants exhibited a slower growth rate and an increase in the cell fraction in the G1 phase under our experimental conditions. These data indicate that C9-p59 has growth inhibitory activity for prostatic epithelial cells. Taken together, our results suggest that C9 is up-regulated during prostate degeneration process and may play an active role in the proliferation and differentiation of prostatic epithelium.


Subject(s)
Adaptor Proteins, Vesicular Transport , Gene Expression Regulation , Orchiectomy , Prostate/metabolism , Prostatic Neoplasms/pathology , Proteins/genetics , Adaptor Proteins, Signal Transducing , Alternative Splicing , Amino Acid Sequence , Animals , Apoptosis Regulatory Proteins , Base Sequence , Cell Division , DNA, Complementary/chemistry , DNA, Complementary/isolation & purification , Genes, Tumor Suppressor , Humans , Kinetics , Male , Molecular Sequence Data , Polymerase Chain Reaction , Proteins/chemistry , Proteins/physiology , Rats , Rats, Sprague-Dawley , Tumor Cells, Cultured , Tumor Suppressor Proteins
13.
Cancer Res ; 57(10): 1868-72, 1997 May 15.
Article in English | MEDLINE | ID: mdl-9157977

ABSTRACT

Our previous studies demonstrated that the promyelocytic leukemia gene, PML, encodes a growth and transformation suppressor. Overexpression of PML inhibits cancer cell growth in vitro and in vivo. In this study, we further explored the possibility of applying PML as a potential agent for developing prostate cancer gene therapy using an adenovirus delivery system. We have constructed and produced the recombinant PML-adenovirus, Ad-PML, in which the full-length PML cDNA is driven by the strong cytomegalovirus promoter. In LNCaP, DU145, and PC-3 prostate cancer cell lines, an infection efficiency of 90% can be achieved at a concentration of 2, 10, and 100 multiplicity of infection (MOI), respectively. Western blotting and immunofluorescence staining demonstrated that the AD-PML-infected cells expressed a high level of PML protein. The protein expression peaked at days 3-4 postinfection, and a detectable level of PML was found at day 18 after viral infection. To test the effect of Ad-PML on the growth of prostate cancer cells, the DU145 and LNCaP cells were infected with 10 and 2 MOI of Ad-PML. We found that the growth rate of the Ad-PML-infected DU145 and LNCaP cells were significantly inhibited. A tumorigenicity test in nude mice showed that the Ad-PML-treated DU145 cells failed to form tumors. Most importantly, direct injection of Ad-PML into DU145-induced tumors was able to repress tumor growth in nude mice by 64%. Taken together, these data indicate that PML is a tumor growth suppressor in prostate cancer and that Ad-PML may be a potential candidate for human prostate cancer therapy.


Subject(s)
Adenoviridae/genetics , Genetic Therapy/methods , Neoplasm Proteins , Nuclear Proteins , Prostatic Neoplasms/therapy , Transcription Factors/physiology , Adenoviridae/metabolism , Animals , Blotting, Western , Cell Division/physiology , Fluorescent Antibody Technique , Humans , Male , Mice , Mice, Nude , Promyelocytic Leukemia Protein , Prostatic Neoplasms/metabolism , Prostatic Neoplasms/pathology , Recombinant Proteins/biosynthesis , Recombinant Proteins/genetics , Transcription Factors/biosynthesis , Transcription Factors/genetics , Transfection , Tumor Cells, Cultured , Tumor Suppressor Proteins
14.
Adv Exp Med Biol ; 250: 273-90, 1988.
Article in English | MEDLINE | ID: mdl-3076326

ABSTRACT

ODC, the first enzyme in mammalian polyamine biosynthesis, is rapidly induced in response to a wide variety of growth stimuli. However, there is no single mechanism which may explain the rapid turnover of ODC activity. ODC activity has been shown to be regulated at the level of synthesis and degradation, and also by post-translational modifications and an interaction with macromolecules. Our results indicate that TPA-induced ODC activity is regulated at the transcriptional level. An initial signal in ODC induction by TPA is not clear. We have suggested that TPA-increased accumulation of epidermal prostaglandins is required, but not sufficient, for ODC induction by TPA. Others have suggested the role of lipoxygenase product(s) in ODC induction. The role of the microtubule-containing system in regulation of ODC induction has been shown. The involvement of cyclic nucleotides in ODC induction by TPA is controversial. Also, generation of free radicals appears to be involved in ODC induction by TPA. Data summarized in this chapter indicate that activation of PKC may be an initial step in ODC induction by TPA.


Subject(s)
Ornithine Decarboxylase/biosynthesis , Protein Kinase C/metabolism , Skin Neoplasms/chemically induced , Tetradecanoylphorbol Acetate/pharmacology , Animals , Enzyme Induction/drug effects , Epidermal Cells , Epidermis/metabolism , Gene Expression Regulation , Humans , RNA, Messenger/biosynthesis , RNA, Messenger/metabolism , Transcription, Genetic , Type C Phospholipases/pharmacology
15.
Cancer Res ; 46(12 Pt 1): 6149-55, 1986 Dec.
Article in English | MEDLINE | ID: mdl-3779635

ABSTRACT

The role of protein kinase C in ornithine decarboxylase (ODC; EC 4.1.1.17) gene expression in primary culture of newborn mouse epidermal cells (MEC) from BALB/c mice and in skin tumor promotion by 12-O-tetradecanoylphorbol-13-acetate (TPA) in female CD-1 mice was determined. A time course and the dose-response curves of ODC induction paralleled that of ODC mRNA induction by TPA in MEC. TPA treatment did not elicit any change in the size of ODC mRNA. The magnitude of ODC induction was proportional to the amount of ODC mRNA increased by TPA. TPA (2 X 10(-7) M) failed to induce ODC activity in MEC plated in Ca2+-deprived medium; TPA induction of ODC could be resumed upon Ca2+ restoration in the medium. 1-Oleoyl-2-acetylglycerol, a membrane-permeable diacylglycerol which activates protein kinase C, induced at the same rate both ODC activity and the amount of ODC mRNA in MEC. Phospholipase C, which releases diacylglycerol from membrane phospholipids, also induced ODC activity; 0.02 units of phospholipase C per ml led to about a 50-fold increase in ODC activity at 6 h after treatment. Phospholipase A2 was ineffective. Phospholipase C-induced ODC activity correlated with an increased level of ODC mRNA. Furthermore, palmitoylcarnitine, an inhibitor of protein kinase C, inhibited epidermal ODC induction and the increased level of ODC mRNA by TPA. Also, palmitoylcarnitine inhibited skin tumor promotion by TPA; application of 3 mumol of palmitoylcarnitine in conjunction with each promotional treatment with 10 nmol of TPA to the initiated skin of female CD-1 mice inhibited tumor formation. Taken together, we conclude that activation of protein kinase C may be an early event in ODC gene transcription and skin tumor promotion by TPA.


Subject(s)
Epidermis/enzymology , Ornithine Decarboxylase/genetics , Protein Kinase C/physiology , Skin Neoplasms/enzymology , Tetradecanoylphorbol Acetate/pharmacology , Animals , Cells, Cultured , Diglycerides/pharmacology , Enzyme Activation , Female , Mice , Mice, Inbred Strains , Ornithine Decarboxylase/biosynthesis , Palmitoylcarnitine/pharmacology , RNA, Messenger/analysis , Skin Neoplasms/chemically induced , Transcription, Genetic , Type C Phospholipases/pharmacology
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